查询词典 subculture

Primary culture and subculture were adopted using lung cells of a female Elaphodus cephalophus' embryo. A new karyotype and a pair of big telocentric chromosomes were found. The diploid has 48 chromosomes, and the karyotype formula is 2M+2ST+42T+XX. C-banding analysis shows that these chromosomes are homologous with those reported relevant chromosomes. This article is the first to report the polymorphism of Elophodus cephalophus B-chromosomes and studies it's transmitted mechanism.

采用原代培养和传代培养法，对1头毛冠鹿的胚肺组织细胞进行观察，发现一种新的核型，其二倍体染色体数目为2n=48，核型公式为2M+2ST+42T+XX，出现1对大的末端着丝粒染色体，C-带显示该染色体与已报道的相关染色体同源，首次提出毛冠鹿B染色体多态，对其传递机制进行了探讨。

We studied the optimum medium and hormone combination of callus induction from cotyledons, subculture of callus, shoot regeneration from callus, shoot-extension induction, root induction and identified the chromosome number of the plantlet. The results showed that: MS+2,4-D 2.0mg/L+KT 0.5mg/L, MS＋2,4-D 2.5mg/L＋KT 0.5mg/L, MS+2,4-D 0+KT 1.0mg/L, MS+KT 0.5 mg/L and basal MS medium. One plantlet among twenty two was determined as a tetraploidy based on the plant morphology, observation of the leaf stoma and chromosome number of the root, other two plantlets represented morphological variation.

结果表明，诱导章丘大葱子叶产生愈伤组织、愈伤组织的继代培养、愈伤组织芽分化、芽点的伸长及诱导生根的最佳培养基及激素配比分别为：MS+2，4-D 2.0mg/L+KT 0.5mg/L、MS＋2，4-D 2.5mg/L＋KT 0.5mg/L、MS+2，4-D 0+KT 1.0mg/L、MS+KT 0.5mg/L和MS基本培养基；试管苗经驯化移栽后，共有22株成活，成活率为92%，对成活的再生植株进行形态学、叶片气孔及根尖染色体鉴定表明，共有3株发生变异，其中2株为外部形态上的变异，而另一株为染色体的加倍变异。

The medium-Nitsch basic medium with 1umol/L 2, 4-D was suitable for the growth of somatic cells. The trimness degree of somatic embryogenesis was more improved after the treatments of cells to be sifted with 1mm diameter screen in 4 days of subculture.

胚性细胞团悬浮培养最适培养基为ND培养基，在继代后4天将细胞团经孔径1mm的钢质筛网多次过滤处理，可以使体细胞胚胎发生的整齐度大大增强。

Alone and untethered, feeling lost in the country he had come to regard as home, Hans stumbles upon the vibrant New York subculture of cricket, where he revisits his lost childhood and, thanks to a friendship with a charismatic and charming Trinidadian named Chuck Ramkissoon, begins to reconnect with his life and his adopted country.

Hans与妻子离异期间，Hans与Chuck结为朋友，小说很大的乐趣来自於二人的交往，Chuck把Hans引到一个他不熟悉的纽约世界。小说一开始是在2006年，Hans与妻子在伦敦重过生活，接到来自纽约警方的电话，告知Chuck的尸体被发现弃於运河；小说便从这悬疑死讯开始，穿梭於回忆与现在之间。

Coculture of autogenic BMSCs with chondrocytes can promote the proliferation of chondrocytes and the production of chondral matrix. Cocultured cells for seeding can save a number of chondrocytes, shorten culture periods and reduce subculture times.

自体BMSCs与软骨细胞共培养，BMSCs能增强软骨细胞的增殖，促进软骨细胞基质合成，缩短软骨细胞培养时间和减少传代次数，可节省大量的软骨细胞，与DBM复合后能有效修复关节软骨缺损。

By this token,induction and subculture of embryonic calli should be related with cooperative effects among different hormones.

由此看来，胚性愈伤组织的诱导和保持，可能还与各种激素的协同作用有关。

In the present investigation, we studied further the growth conditions of the epithelial cells in long-term primary culture and determined the growth capacity of those from 30 senile cataractous lenses with different degree of cataract after the first subculture.

在此次的实验中，我们进一步观察於长期初培养中的上皮细胞的生长情形与检定从30个有不同程度白内障的水晶体取得的上皮细胞在第一次次培养时的生长能力。

In the first subculture, with the increase of cataractous degree, epithelial cells of the lens showed lower attachment ability to culture plates and decreased ability to incorporate 3H-thymidine into newly synthesized DNA, ie, decreased proliferative capacity.

在第一次次培养时，随著白内障程度的增加，水晶体上皮细胞对培养皿壁的黏贴能力及生长中利用培养基中3H-thymidine来合成DNA的能力都显得较差，亦即有较差的增生能力。

By Percoll fluid and density gradient centrifugation,the MSC was obtained;after the cells filled the bottom of vessel,subcultured them,when they subculture in third generation ,redigested them,500 R/min centrifugate,alter the

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞，这种细胞在体外培养体系中保持旺盛的增殖能力；经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化，增殖旺盛，可传一代培养，是软骨组织工程理想的种子细胞。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。