查询词典 subculture

The media and the schools have become mouthpieces for the gay subculture, and are working hard to marginalize those of us who take a moral, biblical stance on the issue.

媒体和学校的传声筒同性恋亚文化，并正努力排斥那些人采取一种道德圣经对此事的立场。

Starting in high school,Ned confesses,he became "infatuated with the drug subculture," using marijuana,LSD and mescaline.

奈德承认，从高中开始，他对"药醉神迷生活"产生迷恋，并且服用大麻、"赖瑟酸二乙基酰胺"及梅斯卡灵等麻醉药。

Fiske, John (1989) Understanding Popler Culture, Boston: Unwin Hyman; Hebdige, Dick (1979) Subculture: the Meaning of Style, London: Methuen; de Certeau, Michel (1988) The Practice of Everyday Life, trans Stephen Rendall, Berkeley: University of California Press; Lefebvre, Henri (1971) Everyday Life in the Modern World, tras.

约翰·费斯克（1989），《理解波普文化》；波士顿： Unwin Hyman 出版社；迪克·海博蒂格（1979），《亚文化：风格的意义》；伦敦： Methuen 出版社；米歇尔·德·塞托（1988），《日常生活实践》；斯蒂芬·伦达尔译，贝克莱：加利福尼亚大学出版社

Nisan is part of a thriving subculture of men and women in Japan who indulge in real relationships with imaginary characters.

尼森现象只是一种在日本男女中间兴起的亚文化，他们沉迷于和虚拟人物的关系之中。

After subculture thrice under nonselective condition, G418 resistance could still inherit stably.

所获得的转化子在无选择压力下连续培养3代，G418抗性仍能稳定遗传。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

After two weeks subculture, the cells can overspread the culture plate, most of which look like shuttle.

在此基础上尝试对人角膜上皮和基质细胞进行原代培养。

The results showed as follows: 3mg/LBA in differentiation medium was favorable for regeneration of callus; addition of 3mg/LABA, sucrose substituted with maltose, or sucrose partially substituted with mannitol in subculture media improved the status of callus in a degree and enhanced the differentiation frequency; partial desiccation treatment significantly increased the regeneration frequency of callus; addition of AgNO3 or CuSO4 in differentiation medium contributed to plant regeneration , The two resistant calli and fifteen regenerated plants were obtained when the calli of mature embryo of 7001S were infected with Agrobacterium carrying bar and CPTI gene.

结果表明：3mg/LBA对分化有利；继代培养基中加入3mg/LABA，以麦芽糖代替蔗糖，或以甘露醇代替部分蔗糖在一定程度上可以改善愈伤组织状态，提高分化率；干燥处理能明显提高愈伤组织分化率；分化培养中添加一定浓度的AgNO3和CuSO4有利于愈伤组织分化。借助农杆菌介导bar基因和CPTI基因对水稻进行遗传转化，获得2块抗性愈伤组织和15棵再生苗。

The results showed as follows 3mgLBA in differentiation medium was favorable for regeneration of callus; addition of 3mgLABA, sucrose substituted with maltose, or sucrose partially substituted with mannitol in subculture media improved the status of callus in a degree and enhanced the differentiation frequency; partial desiccation treatment significantly increased the regeneration frequency of callus; addition of AgNO3 or CuSO4 in differentiation medium contributed to plant regeneration , The two resistant calli and fifteen regenerated plants were obtained when the calli of mature embryo of 7001S were infected with Agrobacterium carrying bar and CPTI gene.

结果表明：3mgLBA对分化有利；继代培养基中加入3mgLABA，以麦芽糖代替蔗糖，或以甘露醇代替部分蔗糖在一定程度上可以改善愈伤组织状态，提高分化率；干燥处理能明显提高愈伤组织分化率；分化培养中添加一定浓度的AgNO3和CuSO4有利于愈伤组织分化。借助农杆菌介导bar基因和CPTI基因对水稻进行遗传转化，获得2块抗性愈伤组织和15棵再生苗。

Put forward the viewpoint of denoting the rigidity of media by peptonization intensity(g/cm2).Ascertained that the peptonization intensity of initial media is 160-170 g/cm2,that of subculture and rooting media is 150~160 g/cm2.4 Experiment on sugar source of media.

培养基琼脂用量计算的依据通过试验，提出用胨力强度(g/cm~2)表示培养基硬度的观点，并确定了初代培养基胨力强度为160～170g/cm~2，继代培养基、生根培养基均为150～160g/cm~2。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。