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The resulting strain, designated YES2MTM1, was transformed with a yeast genomic library. Transformants lost the plasmid overexpressing MTM1 after 5-FOA treatment. Yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and overexpression of some DNA fragments were picked up and candidate suppressor genes were identified. Overexpression of five genes were identified to be able to rescue the growth defect on nonfermentable carbon source. The study will provide reference for MTM1 gene function and screening for suppressor of genes whose deletion result in irreversible damage.

为了避免MTM1缺失造成的不可逆损伤，在野生型酵母中先转入带有MTM1 基因的质粒，再敲除染色体上的MTM1 基因，随后转入基因组文库，再利用药物5-氟乳清酸(5-FOA)迫使细胞丢失表达MTM1基因的外源质粒，再筛选能在非发酵培养基上生长的转化子，通过这种方法筛选发现，POR2等5个基因的过表达可以挽救MTM1 基因缺失造成的非发酵培养基上的生长缺陷，为深入了解MTM1基因的功能提供了线索，对筛选其他造成不可逆损伤的突变基因的抑制基因提供了一条可行的研究思路。

Along with the fast fierce development that calculator apply,the application of network extends continuously,the people request to use these techniques urgently,and to carry on the online examination,the work burden and the exaltation work efficiencies that to release the teacher,also raised the quantity of the examination at the same time,thus make examination gradually notarize,objective,stir up the student's interest in the study more.

随着计算机应用的迅猛发展，网络应用不断扩大，人们迫切要求利用这些技术来进行在线考试，以减轻教师的工作负担及提高工作效率，与此同时也提高了考试的质量，从而使考试趋于公正、客观，更加激发学生的学习兴趣。

"Although in our study the number of patients was small and follow-up short, we believe that HolCTU should be considered as an initial treatment for obliterative post-traumatic urethral strictures," the authors write."The procedure is simple, less traumatic, and less morbid, without a blood transfusion requirement, and the patient will require less time away from work. We do not deny that the core-through procedure has a slightly greater rate of failure compared with open surgery. However, failure of the procedure does not prevent conventional urethroplasty if required."

研究人员表示，虽然在研究中，受试者样本数少且追踪期短，但我们相信HolCTU将会像对创伤后闭锁性尿道狭窄的最初治疗般受到重视，因为这项手术过程简单、伤口较少，同时也较少导致其他病变、不需要输血，且患者较不需要花时间离开工作；我们不否定穿越核心的外科手术与一般外科手术相比，有较高的失败率，但无论如何，手术失败将不会阻碍传统的尿道成形术。

"Although in our study the number of patients was small and follow-up short, we believe that HolCTU should be considered as an initial treatment for obliterative post-traumatic urethral strictures," the authors write."The procedure is simple, less traumatic, and less morbid, without a blood transfusion requirement, and the patient will require less time away from work. We do not deny that the core-through procedure has a slightly greater rate of failure compared with open surgery. However, failure of the procedure does not prevent conventional urethroplasty if required."

研究人员表示，虽然在研究中，受试者样本数少且追踪期短，但我们相信HolCTU将会像对创伤后闭锁性尿道狭窄的最初治疗般受到重视，因为这项手术过程简单、伤口较少，同时也较少导致其它病变、不需要输血，且患者较不需要花时间离开工作；我们不否定穿越核心的外科手术与一般外科手术相比，有较高的失败率，但无论如何，手术失败将不会阻碍传统的尿道成形术。

In this study, isobaric tags for relative and absolute quantification labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) was employed to compare the differential protein expressions in MCF7 cells upon 17β-oestradiol (E2) stimulation or serum deprivation versus in normal conditions. A total of 576 proteins with the confidential level of ≥95% were identified, 26 proteins among the 576 proteins were found to be differentially expressed after E2 stimulation or serum deprivation, and among them 10 proteins were up-regulated and 16 proteins were down-regulated by more than two folds.

利用四重用于相对和绝对定量的等量异位标签(isobaric tags for relative and absolute quantification， iTRAQ)标记结合二维液相色谱-串联质谱(two-dimensional liquid chromatography-tandem mass spectrometry， 2D-LC-MS/MS)对雌二醇(17β-oestradiol， E2)和血清各自以及共同作用对MCF7乳腺癌细胞内蛋白质表达的影响进行了比较，共鉴定到置信度在95%以上的蛋白质576种，其中各组相对于正常培养组的细胞共找到26种差异在1倍以上的蛋白质，其中10种上调，16种下调。

The study sets up a model of a single two-staged supply chain, two sides of which are both oligarchic enterprises.

建立了2阶段单链供应链模型，供应链节点双方均为寡头垄断企业。

Experimental study of co-culture showed that there was a good biological compatibility between fibreframe of PGA and SCS.Compared with SCs on the plane,SCs migrated easily on the stereoframe.The long olivary cell of SC was still trending to wrapping fibre up without neurons.

PGA在SCS生长中具有良好的生物相容性；与平面相比SCS更易在立体网架的纤维上迁移，长橄榄形的SCS有包裹纤维的趋势。

This thesis is made up of two sections,The goal of the first section is to study the new reaction of organozinc reagents and applicability of it in the organic reaction.

本论文主要有两部分构成，前半部分主要工作是对有机锌试剂新反应的研究，考察有机锌试剂在这些反应中的适用性，以扩展有机锌试剂在有机合成中的应用范围。

This study thoroughly inspects reciprocal method and leap-frog method of EDM trigonometric leveling along with relationship between EDM height, ellipsoid height, and orthometric height. An experimental area with 200m, 400m and 600m span intervals were set up with vertical angle error ±1.5〞and EDM error ±（2mm+2×10-6×Smm）.

本研究规划实验区实地针对200m、400m、600m三种测段长，采用之全测站仪之垂直角中误差为±1.5〞，测距中误差为±（2mm+2×10-6×Smm），进行EDM三角高程测量，并对测量方法及观测成果精度进行分析，实际探讨短距离EDM三角高程代替水准测量的可能性。

AIM To simulate the cardiovascular response to head up tilt and to further study the physiological mechanism of orthostatic stress.

目的 仿真头高位倾斜时心血管系统的反应，进一步研究有关立位应激的生理机制。

They go to town. Would you just ask them?

他们在城里你能问他们吗？

Ossein pretreated for a2 weeks period exhibited the maximum% yield incase of bone gelatin, while the yield from intact skin was higher than that realized incase of de-scaled skin.

预处理时间达2星期的骨胶原表现出最高的骨明胶百分产量，然而从未处理鱼皮中得到的产率比去鳞的鱼皮得到的要高。