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Methods: in order to detect the effects of sulindac on cultured skov3, sulindac with different concentration and untreated control were set up. the changes in morphology of skov3 were observed by phase contrast microscopy. mtt colorimetric assay was used to study the effects of sulindac on skov3 cell growth. motility of skov3 was determined by scarification assay.

目的:研究舒林酸对卵巢癌skov3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2(mmp2)、环氧合酶-2(cox-2)表达的影响:方法:将不同浓度的舒林酸加入培养液中观察其对skov3细胞形态的影响,采用mtt法观察舒林酸对skov3细胞生长的影响,通过划痕试验观察舒林酸对skov3细胞运动的影响,利用免疫细胞化学方法研究舒林酸对skov3细胞mmp2、cox-2表达的影响。

Research orientation: major in Chinese characters. Phonology and Scholium especially taking phonology as main orientation with an auxiliary study and collation, experienced at making use of computer to set up database for doing research on the ancient document materials with a wider new road.

科研方向:主要从事文字、音韵、训诂研究,尤以音韵、音义研究为主攻方向,辅之以传统语言文字学文献的整理和研究,在利用电脑建立数据库来处理和研究传世文献的材料方面,有独到的体会和经验,拓宽新路,有很多事情可做。

You scrupulously keep your records up to date and study them to learn from your experiences.

你认真做交易记录,从中学习可以积累经验。

We are, after careful study, scrutiny and sum up the results.

我们是经过细致的调研、推敲而总结出来的结果。

Supported by Freud"s psychological analyses and archetypal criticism of Frye and C. C. Jung, the main body of the thesis firstly analyzes the deep reason for the phantom battle. Charles"s jealousy for James and the phantom pursuit of Hartley take their initial in his transferred "Oedipus complex" towards his aunt. Secondly it presents the artistic means Murdoch employs in the novel, dealing with the language confrontation, and such archetypal images as sea serpent and seals. Lastly, the study reveals how Charles fulfills his spiritual maturity through "down-up experience","the last baptism" and "meditations on stars".

论文主体以弗洛伊德的精神分析理论,弗莱和荣格的原型批评为支点从三个方面着手,首先分析导致查尔斯虚幻之争的深层缘由,阐述查尔斯对詹姆斯的嫉妒和对哈特莉梦的追寻源自对婶婶的"俄狄浦斯"情结;进而重点分析默多克呈现兄弟虚幻之争所运用的艺术手段,其中包括言语对抗,"海怪","海豹"等原型意象分析;最终揭示查尔斯如何通过"坠落经历","最后的洗礼","对星星的冥想"等完成自身的精神成长。

At first,I didn't paid any attention to it as usual, but when I stood up and stretched myself after a long sedentariness, a stream of more than fresh air swarmed into my study.

东风教育集团七中校长周正晓说,学校对这次整治行动非常赞成。

How many quiet nights, in order to prepare each lesson, are you seriously study materials; the number of spare time, in order to transform backward students, but also you are braving the cold heat go home visits; the number of holidays, you give up leisure time with their families , devote themselves to learning, to use all available time to "self-charging."

多少个宁静的夜晚,为了备好每一堂课,是您认真钻研教材;多少个业余时间,为了转化后进学生,又是您冒着严寒酷暑去家访;多少个节假日,您放弃与家人休闲的时间,潜心进修,利用一切可以利用的时间来"自我充电"。

Company's values: Study hard, work hard, set up in studying and working self-confidently, thus gain others' respect.

公司价值观:努力学习,勤奋工作,在学习和工作中建立自信,从而赢得别人的尊重。

First of all, from the design of the contemporary environment of the color change of thinking and starting to study the color in the new concept of performance, from a new sense of color and character changes in the use of summed up the new characteristics of color, such as color-hyun, reflecting the color, Self-luminous color and display aesthetics, graffiti and the new material on the impact of color, and interesting in a modern aesthetic with a new understanding on.

首先,从当代环境设计中对色彩的思考和观念的转变出发,研究色彩在新观念下的表现,从色彩的新感觉与性格的转变总结出色彩运用的新特点,诸如炫色、反射色、自发光色与显示屏美学、涂鸦及新材质对色彩的影响,并对其在现代审美与趣味上进行新的理解。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法,制备出具有生物活性的蛋白质毒素,构建了白喉毒素表达载体pET22b-DT,并转入大肠杆菌BL21(DE3)中诱导表达,以融合表达的His-6作为标签通过亲和层析纯化;表达毒素经蛋白酶酶切后,形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素,并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

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And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时,论述了一体化管理体系构建中的几个主要问题,包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。

If you're huffing and puffing at any point during the run, slow down and make sure you can take deep breathes.

如果你任何时候在跑步过程中气喘如牛,降低速度并且确保你能够深呼吸。