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The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

Newspapers and magazines each have their own specific newspaper principles and purposes, WBR has its own audience, before submission to understand this, WBR understand it the issue of the publication cycle is bimonthly, quarterly, monthly or semimonthly , weekly, WBR was a newspaper, if so, daily newspaper, reported Tuesday, weekly newspaper, or a half months, monthly, WBR coming to understand a variety of newspapers and periodicals which are set up columns, WBR columns are published, what kind of article, WBR may If the press should also look at the history of the journal, WBR have made in recent years to see what kind of article, WBR-control study on the issue of your paper and write the original studies have been written, WBR research, the original published before which such article is from the point of writing, WBR whether your innovation and development articles.

每种报刊杂志都有自己特定的办报方针和宗旨，WBR有自己的读者对象，投稿前必须先对此进行了解，WBR搞清它的发行出版周期是双月刊、季刊、月刊还是半月刊、周刊，WBR如果是报纸的话，是日报、周二报、周报还是半月报、月报，WBR接下来要了解各种报刊都开设了哪些栏目，WBR各栏目都发表些什么样的文章，WBR可能的话还应该了解一下报刊的办刊历史，WBR看看近年都发表过什么样的文章，WBR对照一下你研究的问题以及撰写的论文原来有没有人研究过写过，WBR研究现状如何，原来发表过的此类文章是从哪些角度写的，WBR你的文章有无创新发展。

First, oneself should set oneself up as an example, learn very each homework hard, there is model on the study to take the lead a function.

首先，应该自己以身作则，努力学好各门功课，在学习上起模范带头作用。

objective: to observe the therapeutic effect of integrative medicine therapy on circumferential mixed hemorrhoids.methods: peeling and suturing external hemorrhoid by segments,cut and ligating the dentate line of internal hemorrhoids,injecting xiaozhiling liquid,sitz bath and chinese herbal fumigation and washing after operation.results: all the 78 patients cured without massive hemorrhage,anal stenosis and other complications after operation.the follow-up study a year later shows no recurrence.conclusion: the surgical operation is simple and effective,should be worthily recommended in the clinical application.

目的：观察中西医结合治疗环状混合痔的临床疗效。方法：采用外痔分区段剥离缝合、内痔齿线上小v剪口结扎、消痔灵液注射，术后用中药坐浴熏洗。结果：本组78例全部一次治愈，术后无大出血，肛门狭窄等并发症，1年后随访无1例复发，肛门功能正常。结论：本术式简单有效、治愈率高、并发症少，值得临床推广应用。

Combining the analysis and study on magnetic interface, the authors think that the crust structure here is controlled by "a pile up slopes poker face to face", which is a accepted as the growth model of Bohai gulf basin widely; There is a deep-major fault along northeast in west of the area. Its extension in deep is to southeastward which coincides in most of the faults in this area.

结合该地区航磁异常的分层计算与对比，认为本地区的地壳结构受渤海湾裂谷盆地的衬"倾牌组式构造"所控制，该地区西北部存在有北东向展布的深大断裂，其深部延展与本区大部分已确认的断裂深部延展方向基本一致且朝向东南。

Forming some stander of product during the course of study, and the design could fix up the question on the layer and getting to the aim.GOOD product turn into the really good product.

并在这个过程中形成一套较为科学合理的评定标准，从而使产品设计能够解决好这一层面的问题达到设计的目标与要求，进而使产品真正的能够称之为"好"的产品。

On the basis of statistical analysis to the 15 hypothesis of affecting factors, this research also uses the parallel testing method, which employed by the researchers of UCLES while they investigate the validity of contextualized test questions, for reference to give a making-up study to the affecting factors.

在量化统计研究的基础上，本研究又借鉴英国剑桥大学考试委员会的研究者在研究试题的情境化效度时所使用的对比测试研究方法，对部分影响因素作了进一步的补充研究，以探讨利用统计方法不能得到的一些规律。

To tighten the focus on those early findings, psychologists Prathiba Shammi and Donald Stuss conducted a follow-up study at the University of Toronto.

心理学家普拉席巴·谢米和多纳尔多·斯特斯为进一步统一以前各有关研究文献的观点，在多伦多大学开展了一项后续性实验。

The mission of this International Polar Year project is to uncover secrets of the enigmatic Gamburtsev subglacial mountains that are buried by up to 4 km of ice; to hunt for the oldest ice on our planet; to study subglacial lakes and to discover new clues of past, present and future climate change.

这次国际极地年项目的使命是揭开甘毕采夫冰下山脉的神秘面纱，它被深埋在4公里厚的冰下；搜寻地球上最古老的冰；探索冰下湖并获得过去、现在和将来气候变化的最新线索。

Chapter 2 tidies up and build concept relating to research and theoretical foundation,primarily including systematology, collaborative study theory, symbiotic theory,game theory and so on.

第2章对研究的相关概念和理论基础进行梳理和构建，主要包括区域经济发展理论、核心边缘理论、跨界治理理论、共生理论和博弈论等。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。