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Methods 40 healthy adult Wistar rats were divided randomly into four groups, sham operation group (group A, n=10), torsion/detorsion group (group B, n=10), single astragalus plus detorsion group (group C, n=10), successive astragalus plus detorsion group (group D, n=10). Testicular torsion/detorsion model was established in terms of turner methods.

将40只健康雄性Wistar大鼠分为4组,分别为假手术对照组,睾丸扭转/复位组,睾丸扭转/复位+单次腹腔内注射黄芪注射液组及扭转/复位+连续腹腔内注射黄芪注射液组,每组10只。

Material and Methods:From March,2008 to January,2009,250 patients(146 Males and 104 Females,ranged from 18 to 82 years old,mean age was 48 years old) enrolled in the First Affiliated Hospital of Kunming Medical College were performed dual energy scan(Group A,200 patients) and Neuro-DSA scan(Group B,50 patients) using DSCT.The patients were randomly divided into Group A and Group B.Regarding to the justification of scan protocol,100 patients in Group A were selected as sub-group to explore the scan protocol.The image quality Was analyzed both in four categories(4.0/60、4.5/60、4.0/70、4.5/70) of flow rate and total amount of contrast-enhanced material and in three categories(100—150HU、150—200HU、200-250HU) of CT value of left common carotid artery.The comparison of two scan approach was based on image quality,radiation does, postprocessing methods,time of scanning and subtraction and data storage amount.To validate the diagnostic capability of DE-CTA virtual unenhanced images,100 patients in Group A were undergoing head scan both in routine non-contrast program and DE-CTA virtual unenhanced program.Mean CT values,signal-to-noise ratio,image quality,sensitivity of lesion identification,and radiation does were applied to comparison.According to diagnostic results of dual energy of cerebral arterial angiography,62 patients out of 200 patients in Group A were confirmed with cerebral vessel lesions,the results were complied with DSA findings(the time between the two methods were within 1 week).

材料与方法收集2008年3月到2009年1月间在昆明医学院第一附属医院行DSCT CTA检查者250例(男性146例,女性104例,年龄范围18-82岁,平均48岁),随机分为A、B两组,A组200例行双能量头颅CTA扫描;B组50例行Neuro-DSA扫描。A组前100例按对比剂不同流率和总量分为4组(4.0/60、4.5/60、4.0/70、4.5/70)对图像质量比较;按触发时左颈总动脉内平均CT位分3组(100—150HU、150—200HU、200-250HU),比较图像质量的差异;对A、B两组血管图像质量、辐射剂量、后处理的方法、扫描和后处理时间及数据的容量进行比较分析;A组中前100例行常规与DE-CTA虚拟平扫的平均CT值、信号噪声比、图像质量、病灶显示情况、辐射剂量的比较分析;A组中行DSA检查62例(两种方法间隔时间均1周内),比较两种检查方法诊断结果是否符合,并对其中24例动脉瘤行动脉瘤部位、数目、形态、瘤颈显示程度、瘤颈大小及瘤体长径和短径的比较研究。

The effect of Jiusuyu on ebriety rate, ebriety time of acute alcoholism mice observed after preventing treatment 80 healthy mice, bisexual and each half, were randomly divided into 4 groups, 20 mice every group, namely model group (equal volume physiological saline were given through the means of watering stomach), low-dose group of Jiusuyu (called low-dose group, 1.62g/100g avoirdupois Jiusuyu drank through the means of watering stomach), high-dose group of Jiusuyu (called high-dose group, 3.24g/100g avoirdupois Jiusuyu drank through the means of watering stomach), and Gehuaji-echeng liquid control group (called control group, 1.31g/100g avoirdupois) Gehuajiecheng liquid were drunk through the mea

酒速愈预防性给药对急性酒精中毒小鼠醉酒时间、醉酒率的影响健康昆明种小鼠,雌雄各半,随机分为4组:模型组、酒速愈低剂量组(灌服酒速愈1.62g生药/100g体重)、酒速愈高剂量组(灌服酒速愈3.24g生药/100g体重)、葛花解酲汤组(灌服葛花解酲汤1.31g生药/100g体重),每组20只。各组小鼠禁食12hs,各治疗组均一次性给药,模型组灌服生理盐水,30mins后均以56°红星二锅头酒灌胃(1.4ml/100g体重)。观察记录小鼠醉酒时间(从清醒到翻正反射消失的时间),小鼠醉酒与否以翻正反射是否消失为标准:小鼠灌酒后将其背向下轻轻放入鼠笼,若小鼠背

In bitao group; Zhubo 3, Zhubo 6, Luogehongye etc. in red leaf peach group; Dahongpao, Wuyuexian, Qinglingdongtao, Yixianhong, Yangquanroutao, Datiantao, etc. in crisp peach group; Qiumi, Taiyuanshuimi, Shenzhouhongmi, Wenzhoushuimi, etc. in honey peach group; Okayamahaku, Spring time, Rebin, Hakuho, Xiahui No2, Zhaoyian, Fenghuayulu, etc. in juicy peach group; Yulupantao, Wuyuexianbiangan, Sulianpantao, Jiaqing, Yangzhou 124, etc. in flat peach group; Shuguang, Ruiguang No.2, Mayfire, Aimila, Hongliguang, Armking, etc. in nectarine group; Fertinimoroteini, Xizhuang No.1, Nong 1-2-4, Gold queen, Red haven, Jinxiu, Chengxiang, Elberta, Jingcheng, etc. in yellow peach group.

从多态带、特殊位点对类群分析,提出了各类群核心种质,砧木类有列玛格哈露红及贝蕾、阿克拉娃;寿星桃有红重瓣、寿星桃,寿粉,白单瓣;碧桃有人面桃,红碧桃、红花碧桃;垂枝桃有垂枝桃、鸳鸯垂枝、垂枝桃;红叶桃有筑波3号、筑波6号和洛格红叶;硬肉桃有大红袍、五月鲜、秦岭冬桃、敦煌冬桃、一线红、阳泉肉桃和大甜桃;蜜桃有秋蜜、太原水蜜、深州水蜜和温州水蜜;水蜜桃有冈山白、Spring time、Rebin、白凤、霞晖2号、早艳、奉化玉露;蟠桃有玉露蟠桃、五月鲜扁干、苏联蟠桃、嘉庆、杨124;油桃有曙光、瑞光2号、五月火、艾米拉、红李光、阿姆肯;黄肉桃有爱保太,佛尔蒂尼莫蒂尼、金皇后、Red haven、西庄1号、金橙、橙香和锦锈。

Methods]By observing the cytoplasmic Ca2+ concentration change of single cardiocyte in I/R status and the effect of Na+/H+ permutoid retarder to Ca2+ concentration in different phases (diabetes pure oxygen-lack deficit group called DM group,the oxygen deficit/reaeration on entire journey for medicine group called DM-EIPA group, the first group giringmedicine before reaeration on called DM-EIPA 1 group, and the second group giringmedicine before reaeration on called DM-EIPA 2 group) to detect the protection mechanism of Na+/H+ permutoid retarder to diabetic mouse cardiac muscle cell oxygen deficit/reaeration injury.

方法] 通过观察糖尿病鼠单个心肌细胞在缺氧/复氧时细胞胞浆Ca2+浓度的动态变化以及Na+/H+交换体阻滞剂在不同时相(糖尿病单纯缺氧组即DM组,缺氧/复氧全程给药组即DM-EIPA组,复氧前给药1组即DM-EIPA 1组,复氧前给药2组即DM-EIPA 2组)对Ca2+浓度的影响来研究Na+/H+交换体阻滞剂对糖尿病鼠心肌细胞缺氧/复氧损伤的保护机制。

12H treatment group compared with 12h model group: the level of SOD, MDA of the Gardenoside group is no different with the model group; the level of the SOD of the Baical in group increase and the level of MDA is no different; the level of SOD of the Concha Margatitifera Usta is no different and the MDA of it decreased than the model; the level of SOD of the Cholic Acid group increased and the level of MDA decreased; the level of SOD of the Hefang Group increased and the level of MDA of it decreased than the model group.

考虑nNos可能只是参与缺血早期的神经毒性作用。到缺血24小时这一时程已经减低或耗竭。大鼠局灶性脑缺血12小时模型组缺血脑组织P53光密度值含量较正常组明显增高(p<0.01)。珍珠母治疗脑缺血12小时组缺血脑组织P53光密度值较缺血12小时模型组明显降低。胆酸治疗脑缺血12小时组缺血脑组织P53光密度值较缺血12小时模型组明显降低(p<0.05)。栀子苷治疗脑缺血12小时组缺血脑组织P53光密度值较缺血12小时模型组明显降低(p<0.05)。

The rabbits were feeded by estradiol valerate everyday as follows. Group A: E〓V 1. 8mg/d, group B: E〓V 3. 6mg/d, group C: E〓V 7. 2mg/d, group D: E〓V 3. 6mg/d+MPA 3. 6mg/d, group E was the untreated control group, group F was the untreated sham control group.

之后给予口服雌激素或联合应用孕激素共16周,给药方案如下:A组〓V 1.8mg/天,B组〓V 3.6mg/天,C组〓V 7.2mg/天,D组〓V 3.6mg/天+MPA 3.6mg/天,E组和F组均不用药。

Methods 120 healthy Wistar rats were randomly divided into 6 groups after adaptative feeding for one week: 4month control (4C) group, 14month control (14C) group, 14month JL (14JL) group, 24month control (24C) group,24month JL (24JL) group and (1424)month JL (1424JL) group, 20 rats per group, male and female were half to half in every group.

选用Wistar大鼠120只,适应性喂养1 w后随机分为6组:4月龄空白对照组(4C组)、14月龄空白对照组(14C组)、14月龄加味青麟丸组(14JL组)、24月龄空白对照组(24C组)、24月龄加味青麟丸组(24JL组)和14~24月龄加味青麟丸组(14~24JL组),每组各20只,雌雄各半。各组均按实验设计要求分别喂养后剖检。

Full-term pregnancy of primipara 120 cases of illness; Stochastic double divides into three groups: research of group-A group and B group. control group of C group, A group (n=40), 0.75% levobupivacaine 10mg for CSEA, B group (n=40), 3% chloroprocaine the hard membrane pericoel hinders, C group: 0.75% bupivacaine for under the arachnoidea the cavity hinders the anaesthesia, In surgery process, to A and B groups, 2% lidocaine were intermittently injected respective-ly by epidural cathete, effec not enough of anesthesia.

足月初产妇120例随机双盲分成三组:研究组为A组和B组,对照组为c组。A组(n=40)蛛网膜下腔阻滞麻醉用药为0.75%左旋布比卡因10mg,使用腰麻硬膜外联合阻滞行剖宫产术,B组(n=40)以3%氯普鲁卡因为硬膜外麻醉的局麻药,使用硬膜外麻醉阻滞行剖宫产术,c组(n=40)腰麻用药为0.75%布比卡因10mg,使用腰麻硬膜外联合阻滞行剖宫产术。

Results: Neonatal rats in hypoxic-ischemicgroup behaved restlessness, cyanosis, deep and rapid breath, astasia, lethargy, irritation and spasm; There were local pyknosis, fragmentation, dissymmetry, blur or disappearance of nucleoli, raritas of ground substance in left cerebral cortex; but there was no statistic significance in chinese traditional medicine group and sham group. The expression of HIF-1α mRNA in brain tissue of experimental group and chinese traditional medicine group were higher than that in sham group, especially chinese traditional medicine group at 3d; HIF-1α positive cells were found in cerebral cortex and hippocampus. There were more HIF-1α positive cells in experimental group and chinese traditional medicine group and mainly in vascular endothelial cells.

结果 缺氧缺血后大鼠相继表现为烦躁不安、全身发给、呼吸加深加快、站立不稳、嗜睡、激惹或间断发作的痉挛和抽搐;HE染色显示实验组大鼠左侧大脑皮层出现局灶性神经元核固缩,核碎裂,核仁偏位,不清或消失,基质疏松;中药组大鼠脑组织结构基本接近假手术组,无明显水肿和坏死表现;实验组和中药组大鼠脑组织HIF-1α mRNA表达较假手术组明显增加,尤其是中药组实验第3日者;免疫组化显示实验组和中药组各时间点大鼠大脑皮层和海马区均可见不同程度的HIF-1α表达,明显高于假手术组,阳性表达主要在血管内皮细胞,海马和皮层的锥体细胞亦有HIF-1α阳性细胞分布。

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