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The expressed protein concentrates were about 0.5-5mg/L. 3. Classification and functional annotation of kinases have been studied: according to the kinases Classification in PlantsP (http://plantsp.genomics.purdue.edu/), 27 kinases belong to 7 groups in the class 1 of Transmembrane Receptor Kinase and Related non-Transmembrane Kinases on PlantsP, namely Receptor like cytoplasmic kinaseⅦof group 1.2, CRPK1 Like Kinase of group 1.3, Crinkly 4_Like kinase of group 1.4, Plant Extemal Response Like Kinase of group 1.6, CRPK1 Like Kinase (Types 1 and ) and S-Domain Kinase (Type 2) of group 1.9, Legume Lectin Domain Kinase of group 1.11, Wall associate kinase of group 1.5, respectively. 4. 21 kinases expressed in S.

对获得的蛋白激酶进行了分类和功能预测:按照PlantsP网站(http://plantsp.genomics.purdue.edu/)的激酶分类系统,本实验表达的激酶分别属于第一类跨膜受体激酶和无跨膜区的激酶中的7个不同组,即第2组的Receptor LikeCytoplasmic KinaseⅦ家族、第3组的CRPK1L(Type 1)家族、第4组的Crinkly 4_Likekinase家族、第6组的Plant External Response Like Kinase家族、第9组的CRPK1 LikeKinase(Types 1 and 2)和S-Domain Kinase(Type 2)家族、第11组的Legume LectinDomain Kinase家族和第17组的Wall Associated Kinase kinase家族; 4。

Methods: 27 SD rats were randomly divided into 3 groups: normal oxygen group, hypoxia group and combinational gaoyuanchun grain group (The following named remedy group). Put remedy group and and hypoxia group to decompressure chamber on time, decrease pressure to 0.06MPa, moved them out of decompression chamber 5 hours latter, and determined rats' brains oxygen freee radical changes of each group.

将SD大鼠27只随机分为常氧组、低氧组和复方高原春颗粒组,将药物组和低氧组按时入低压舱减压至0.06MPa,5h后减压出舱,测定每组大鼠脑匀浆氧自由基的变化。

There were 8 groups and each groups had 8 cows in the experiment.groups A was the positive group, be feeded basic dietary and in the normal environment; groups B was basic dietary and in the improved environment ; Group C was feeded with basic dietary and NaHCO_3(120g/d/head) and Mg0(50g/d/head) and KCl(180g/d/head), in the environment with no spray and no wind; Group I is feeded with basic dietary and NaHCO_3 (120g/d/head) and MgO(50g/d/head) and KC1 (180g/d/head) and in the another environment with spray and wind; Group E was feeded,with basic dietary and traditional druggery additive in the environment with no spray and no wind; Group F was feeded with basic dietary and traditional druggery additive in the environment with spray and wind;Group G was feeded with basic dietary and NaHCO_3 (120g/d/head) and MgO(50g/d/head) and KCl (180g/d/head) and traditional druggery additive in the environment with no spray and wind; Group H was feeded with basic dietary and NaHCO_3( 120g/d/head ) and Mg0(50g/d/head) and KCl(180g/d/head) and traditional druggery additive in the environment with spray and wind.

试验分8个处理,每个处理8头奶牛,A处理为对照组,喂以基础日粮,处于常规环境(未喷雾,未机械吹风,下同),B组为喂以基础日粮,处于改善环境:C组为基础日粮+NaHCO_3(120克/头。日)+MgO(50克/头。日)+KCl(180克/头。日),处于常规环境:D组为基础日粮+NaHCO_3(120克/头。日)+MgO(50克/头。日)+KCl(180克/头。日),处于改善环境;E组为基础日粮+中草药添加剂组,处于常规环境;F组为基础日粮+中草药添加剂组,处于改善环境;G组为基础日粮+NaHCO_3(120克/头。日)+MgO(50克/头。日)+KCl(180克/头。日)+中草药添加剂组,处于常规环境;H组为基础日粮+NaHCO_3(120克/头。日)+MgO(50克/头。日)+KCl(180克/头。日)+中草药添加剂组,处于改善环境。

Results (1) Compared with that of 4-Retro-decoction group and heart failure control group, the activity of Na〓-K〓-ATPase in low- and middle- dosage ginger group showed to be decreased with statistically significant difference. P<0.05.(2) The activity of Ca〓-ATPase and Mg〓-ATPase in the lower- and middle-dosage ginger group were not statistically different from that of 4-Retro-decoction group and control group, but the activity of Ca〓-ATPase showed an ascending tendency in low-dosage ginger group.

结果 (1)低、中剂量干姜组的Na〓-K〓ATP酶活性明显偏低,与四逆汤组和心衰组比较,差别均具有显著性,P<0.05;(2)低、中剂量干姜组的Ca〓-ATP酶和Mg〓-ATP酶活性与四逆汤组和心衰对照组比较,无统计学意义,但低剂量干姜组的Ca〓-ATP酶与其它三组相比有升高趋势。

In the group of treatment with ZKK,the AST,ALT and the lipid in the liver decrease significantly and differ from the natural recovery group'sbut the normal group's.In the group of treament with Simvastatin,the affection to the ALT,AST and the lipid in liver is no apparent,there is no difference campared to that of recovery group,but very difˉferent from that of treatment group with ZKK,In the combined treatment group,the effect to decrease the bloodlipid is significant,the influence on liver is similar to the ZKK's.Conclusion Rich-fat food can induce dyslipidemia,atheromatous patch and hepar adiposum.

辛伐他汀组对成模时增高的ALT、AST和肝组织中的脂质及脂肪肝影响不大,与模型组相比无差别;之可康组和联合治疗组均可明显降低成模时升高的AST和ALT及肝组织中的脂质,逆转脂肪肝,与正常组相比无差别,与模型组、辛伐他汀组分别相比差异有显著性;联合用药组血脂水平调节幅度最大,对肝脏的影响与之可康组相比无差别。3个治疗组均可使动脉硬化斑块回缩。

On the 3 rd day after operation, compared with group C, the latency period and swimming distance of group O, group Hup extended further, while compared with group O,the latency period and swimming distance of group Hup shortened (P.05);Compared with group C, the extending degree of the latency period and swimming distance of group O decreased on the 7 th day after operation.

结果 术后1 d进行水迷宫测试,与C组比较,A组、O组潜伏期和游泳距离均延长(P.05);术后3 d,与C组比较,O组、Hup组潜伏期和游泳距离进一步延长,而Hup组与O组比较,潜伏期和游泳距离均缩短(P.05);术后7 d,O组与C组比较,潜伏期和游泳距离延长程度缩小。

For instance purchase equipment unit: Qinghua University, Shandong University, Shandong agricultural college, Hebei Province agricultural college, Shanghai medical college, Beijing capital medical college, Nanjing medical college, Wuhan chemical industry institute, Wuhan University of Science and Technology, Beijing Friendship Hospital, In the Suzhou nonmetalliferous ore research institute, Xinjiang the base natural purification high technology and new technology research institute, the Nanjing main source science and technology research institute, the Tianjin chemical industry research design institute, the Heilongjiang Province chemical industry research institute, the Hainan Province institute of pharmacology, the Chongqing treasure Xin group, the broad state established white group, the mansion Mungu dragon group seasoning company, in the Hsuchou graciousness China 药业 group, Kunming the friend group new material company, the Zhejiang Conn shell raises the face hall 药业 company, association abundant group Guizhou 药业, mountain Western Jin Dynasty new double crane 药业, Beijing double lucky 药业, Shanghai double-base 药业, the Shenzhen Jin'gang source new material science and technology development company, the North Sea fruit fragrant garden fruit juice limited company, the Jiangsu three instruments of torture aloe limited company, lucky fresh big Ze The spiral algae limited company, the rich farmer chemical industry company, the Hong Kong 沐 forest food industry group, the Jake mho industrial corporation, Ai Mote the company the Shanghai Kazak chemistry technology limited company, brightly reaches the labor trade limited liability company suddenly, the Jiangsu strength guarantees the building materials industry limited company, the Shanghai China valuable peacock essence spice limited company, user and so on Chongqing Asia mother substance technology company.

比如采购设备单位:、清华科技园股份有限公司,苏州非金属矿研究院、天津化工研究设计院、黑龙江省化工研究院、南京医科大学、武汉化工学院、武汉理工大学、海南省药物研究所、重庆钰鑫集团亚原生物技术公司、广州立白集团、厦门古龙集团、徐州恩华药业集团、南京蓝本科技研究所、河北省农业大学、昆明中友集团新材料公司、浙江康恩贝养颜堂药业公司、深圳金刚源新材料科技发展公司、北海果香园果汁有限公司、江苏三木芦荟有限公司、福清新大泽螺旋藻有限公司、富农化工公司、利农化工有限公司香港沐林食品工业集团、杰克姆工业公司、菲律宾国际医药公司、上海哈勃化学技术有限公司等用户。

Result:As compared with the model group, the effect of small dose group and middle dose group of osteopractic total flavone on TBV% of shankbone increased significantly; the effect of small dose group and middle dose group of osteopractic total flavone on TRS% of shankbone reduced significantly and TFS%, AFS%, MAR, BFR of shankbone reduced obviously; the effect of middle dose group of osteopractic total flavone on OSW and mAR reduced obviously, the effect of small dose group of osteopractic total flavone on them had the tendency of reducing, but there was no statistical significance.

结果 :对胫骨骨小梁体积百分比的影响,与模型组比较,骨碎补总黄酮小剂量组和中剂量组的TBV %明显增高;对胫骨骨小梁吸收表面百分比的影响,与模型组比较,骨碎补总黄酮小剂量组、中剂量组的TRS %显著降低;对胫骨骨小梁形成表面百分比,活性生成表面百分比,骨小梁矿化率和骨小梁骨生成率的影响,骨碎补总黄酮小剂量组、中剂量组的TFS %,AFS %,MAR ,BFR较模型组皆明显降低;对胫骨类骨质平均宽度和骨皮质矿化率的影响,骨碎补总黄酮中剂量组的OSW和mAR与模型组比较,明显降低;骨碎补总黄酮小剂量组与之相比,有下降的趋势,但无统计学意义。

UA of blood of group of model Ⅰ,Ⅱ is elevatory (P.01), and UA of serum of group of Ⅰ of model of; of above of tenability 48 H is in 6, 24, 36, 48 H all group of Ⅱ of prep above model, model Ⅱ group is in 24, 36, group of Ⅲ of model of 48 H prep above (value of UA of serum of group of Ⅲ of model of P.05,.01); is in after building a model 6, 12, 24, 36 H apparent prep above contrasts group (P.01), it is normal that 48 H restores to come.

模型Ⅰ、Ⅱ组血UA升高(P.01),且可维持48 h以上;模型Ⅰ组血清UA在6、24、36、48 h均高于模型Ⅱ组,模型Ⅱ组在24、36、48 h高于模型Ⅲ组(P.05、。01);模型Ⅲ组血清UA值在造模后6、12、24、36 h明显高于对照组(P.01),48 h恢复至正常。

Methods Ninety Sprague-Dawley rats were divided into six groups randomly (15 rats in each group): sham group, cerebral ischemia reperfusion group, high dose Pu-huang Recipe group, medium dose Pu-huang Recipe group, low dose Pu-huang Recipe group, Nao-xueshuan troche group.

实验采用90只清洁级SD大鼠,随机分为6组(每组15只):假手术组,模型组,复方蒲黄大剂量组,复方蒲黄中剂量组,复方蒲黄小剂量组,脑血栓片组。

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