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All the indexes were tested in 2 -. 4 .. 6 weeks of operative intervals respectively. Results:(1) The general observation in the experimental group ,such as spirit, hair, body weight and activity ,was superior to that in the control group.(2) The recovery rate of sciatic nerve functional index (SF1) was statistically significantly higher in the experimental group than in the control group .(3) electrophysciological indexes: m the experimental group ,the tardy rate of induced motorius action potential ,recovery rates of the amplitude of compound muscle action potential and twitch tension and tetanic tension of triceps surae muscle ,were significantly higher than in the control group.(4) Compared with the control group ,the triceps surae muscle wet weight was significantly greater in the experimental group.(5) histomorphological indexes: the count of myelinated nerve fibers ,diameter of regenerated axon, thickness of nerve myelin sheath and area of capillary were much more in the experimental group than in the control group.(6) observation of ultrastructure with the transmission election microscope: more abundant organell a and maturer myelin sheath lay in the injured peripheral nerve of the experimental group, while not lay in the control group.

分别在木后第2、4、6周时进行各项指标的检测,结果发现:(1)实验组SD大鼠在整个实验过程中,其精神、毛发、体重,运动灵活性等一般情况优于对照组;(2)实验组的坐骨神经功能指数恢复率显著优于对照组,P<0.01;(3)神经电生理指标:实验组运动神经诱发电位潜伏期的延迟率显著优于对照组,P<0.05;复合肌肉动作电位振幅的恢复率显著高于对照组,两组比较第2周时P<0.01,第4、6周时,P<0.05;小腿三头肌单收缩力和强直收缩力的恢复率实验组显著高于对照组,P<0.01;(4)小腿三头肌湿重的恢复率,第2、4周时实验组高于对照组,差异非常显著,P<0.01;第6周时,差异仍显著,P<0.05;(5)组织形态学指标:有髓种经轴突计数、再生轴突直径的恢复率,毛细血管面积,实验组均显著大于对照组,P<0.01、P<0.05、P<0.001;实验组髓鞘厚度的恢复率也显著高于对照组,第2周时P<0.01,第4、6周时,P<0.05;(6)透射电镜超微结构观察:实验组再生轴突中细胞器丰富,髓鞘结构成熟,神经再生情况优于对照纽。

The first part is preface, on the basis of expounding the background, the purpose, the meaning and the thinking of the thesis study, briefly introduce the research achievements of domestic and international urban spatial structure; the second part is summary, expatiate on the connotation, component, research span and space type of urban spatial structure; The third part is theory foundation, mainly introduce the three classical models of city spatial structure in the west country (concentric circles mode, fan-shaped mode and multi-core mode) and the relative city development theories (growth pole theory, dot-axis theory, core-fringe theory, metropolis circle theory and city space evolution theory); The fourth part is general situations of study region; The fifth part is the analysis of Lanzhou city spatial structure evolution, along the historical venation Of city development, on the basis of analyzing industrial structure change, focus on and sumup the characteristics of spatial development and form, meanwhile, analyze factors which influences the spatial development; The sixth part is the analysis of Lanzhou city spatial development trend, put forward the development models of Lanzhou city spatial structure from three space aspects(the core city, the city, region) in the future, they are group gobbet structure, fingers structure, circle structure; The seventh part is to mainly put forward the development strategies for the different spatial structure models.

第一部分为前言,在阐明论文研究背景、目的及意义、思路的基础上,简要介绍了国内外城市空间结构相关研究成果;第二部分为概述,对城市空间结构的内涵、组成要素、研究尺度及其空间类型作以介绍;第三部分为理论基础,主要是对西方城市空间结构的3大经典模型(同心圆模式、扇形模式和多核心模式)以及与本研究相关的城市空间发展理论(增长极理论、点轴理论、核心——边缘理论、都市圈理论和城市空间演化理论)的介绍;第四部分为研究区概况,主要是对研究区域范围的界定及其社会经济发展概况介绍;第五部分是兰州城市空间结构演化分析,顺着兰州城市发展的历史脉络,在分析产业结构变动的基础上,着重对城市空间发展及其形态特征进行归纳总结,并分析了空间发展的主要影响因素;第六部分是兰州城市空间发展趋势分析,从中心城市、市域、区域3个空间层面上提出未来兰州城市空间结构的不同模式,即组团结构、指状结构、圈层结构;第七部分是城市空间发展策略,针对不同的城市空间发展模式提出一些对策建议。

Result The inhibitory effect on pulmonary metastasis of transplanted tumor were 46.36%, 32.73%, 18.2% in the Runing granule preparation group, warming kidney group, dispersing liver and promoting blood circulation group respectively. There wasn't significant difference between Runing granule preparation group and CTX group (50.91%). The expression of VEGF was dropped in Runing grannie preparation group and dispersing liver and promoting blood circulation group. The expression of TIMP-1mRNA was up-regulated in Runing Granule group and warming kidney group. The expression of MMP-9 was dropped in Runing granule preparation group and warming kidney group.

乳宁冲剂组及拆方温肾组、疏肝活血组对裸鼠乳腺癌移植瘤的肺转移抑制作用分别为46.36%、32.73%、18.2%,乳宁冲剂组作用与CTX组(50.91%)比较无显著差异;而乳宁冲剂组、疏肝活血组均可下调肿瘤VEGF蛋白表达;乳宁冲剂全方、温肾方可明显使TIMP-1mRNA的表达升高,MMP-9表达下降;对MMP-9/TIMP-1蛋白表达比值影响以温肾组下降最明显。

24H treatment group compared with 24h model group: the density of light of HSP70 of the group increased and which of TGF-β〓, is not different with the model group; the density of the light the HSP70 of the Baicalin group increased and which of it the TGF-β〓, increased with the model group; the density of light of the Concha Margatitifera Usta is not different and which of TGF-β〓, increased with the model group; the density of light of the Cholic Acid group is not different and the density of light of it of TGF-β〓 increased with the model group; the density of light of the Hefang Group is not different and which of TGF-β〓 is not different than the model group.

合方治疗脑缺血12小时组缺血脑组织TGF-β1光密度值较缺血12小时模型组无显著性差异(p>0.05)。本实验结果发现,与单纯缺血12小时、24小时大鼠相比,药物干预组缺血侧大脑皮层TGF-β1免疫反应明显增强,计算机辅助图像半定量分析两组间缺血侧大脑皮层TGF-β1免疫阳性细胞数差异非常显著。说明药物对局灶性脑缺血损伤可能有一定保护作用,其机制可能与诱导脑细胞TGF-β1合成增加有关。然而,药物调控TGF-β1表达,其促TGF-β1表达增强的机制可能是这样的:脑缺血时TGF-β1mRNA表达增加可能与缺氧诱导神经和胶质细胞刺激生长因子表达有关。

METHODS: 40 rats were randomly divided into four groups:normal group, asthenic spleen group, high-dosage group and low-dosage group with 10 rats in each group, the asthenic spleen rat models were established in group B, C group as well as group D. Rats in group B, group C, group D were fed with normal distilled water, high-dosage of Guizhi decoction and low-dosage of Guizhi decoction respectively, NFAT mRNA, IL-4 mRNA, IFN-γmRNA were determined by PCR.

40只大鼠随机分为空白对照组、脾虚组、桂枝汤大剂量组和桂枝汤小剂量组等4组,每组10只;用规范的脾气虚证大鼠模型造模方法将B组、C组、D组大鼠造模,B组、C组、D组分别用蒸馏水、桂枝汤大剂量、桂枝汤小剂量灌胃,标本采集后用RT-PCR检测NFAT mRNA,IL-4 mRNA和IFN-γ mRNA表达水平。

Adult rats are divided into control group, intrapericardial injection group and negative group, sublingual vein injection group and negative group. 6ds after transfection, issues of heart, liver, lung and kidney are stained with X-gal to observe transfection to myocardium and non-target transfection. 3. CaN Aβ gene silencing in vivo. Adult rats are divided into Control group, hypertrophy model group, intrapericardial injection group and negative group, sublingual vein injection group and negative group.

二整体动物水平上探讨心肌有效转染途径:以PLacZ为报告基因,将成年大鼠分为空白对照纽(生理盐水心包腔内注射后超声导入);转染质粒心包腔内注射组及其阴性对照组:质粒+微泡+酶的混合物心包腔内注射,超声导入;转染质粒舌下静脉注射组及其阴性对照组:质粒+微泡混合液经舌下静脉注射,超声导入。

Methods: Wistar rats were divided into normal group, model group, TCM group Ⅰ, TCM group Ⅱ and western medicine group. In addition to the normal group, the other four groups were reproduced animal model of pulmonic fibrosis. The model group were given normal saline gavage, The TCM group Ⅰ given Feifltkang Mistura (1g/ml) gavage, The TCM group Ⅱ were given Fe4fukang Mistura (0.5g/ml) gavage, WM group were given prednisone gavage. Half of each group were taken blood to test in 28th, 56th day.

选择Wistar大白鼠随机分为正常组、模型组、中药Ⅰ组、中药Ⅱ组、西药组,除正常组外,其余四组均复制肺纤维化动物模型;模型组用生理盐水灌胃,中药Ⅰ、Ⅱ组分别用高、低剂量肺复康合剂灌胃,西药组用强的松灌胃;各组分别检测血清相关指标。

With the electrical connections at the top and bottom side of the wafer-level IC packaging Lead-frame structure and composition of the surface-mount semiconductor package structure Multi-layer printed circuit board Antifuse and its formation method and with the anti-fuse non-volatile memory device unit cell Tandem electric signal processing circuit and electronic device Light-emitting diode packaging structure and encapsulation method Electronic Packaging Structure Flip-chip high-speed optoelectronic components and structure Pairs of piezoelectric friction side by side to promote the three-step device and scanning probe microscope Light-emitting diode and its manufacturing method, the production base of light-emitting diode method Three or four parallel advance of stepping piezoelectric device and scanning probe microscope lens body Silicon substrate and its manufacturing method Semiconductor device and voltage-divider A polysilicon layer and the microcrystalline silicon layer of the double-substrate active layer structure, methods and devices The edge of the thickness of silicon controlled Of a lateral semiconductor devices and high-voltage devices With a vertical-channel transistors semiconductor device Of a memory array and for the manufacture of a memory array method Read-only memory cell array structure Active-matrix substrate and display device High-voltage semiconductor integrated circuit devices, dielectric isolated type semiconductor device Image sensing devices Lens module and its manufacturing methods Solid-state imaging device and camera Injection angle for the trench isolation Organic Light-Emitting Display Device Organic light-emitting display device Bipolar transistor structure of the surface passivation Double-triggered silicon-controlled rectifier HFET Metal-oxide semiconductor transistors Self-aligned trench accumulation mode field effect transistor structure Thin-film transistors and Display Devices TFT Lead Diode Low-frequency, low noise, low-flashing diode Used for thin-film solar cells trap light structure Transparent sun solar cells Quaternary semiconductor heterojunction photovoltaic cells heat Si nano-pillar array heterojunction thin-film solar cells GaN-based micro-composite solar cells isotope Optical sensor Semiconductor by optical components Imaging Detector Transparent conductive oxide coating Silicon-based high-performance dual-junction solar cells Thin-film solar cells Alien LED Devices

非专业,不在行,求高手帮忙。谢谢!具有顶部及底部侧电连接的晶片级集成电路封装导线架结构及其构成的表面黏着型半导体封装结构多层印刷电路板反熔丝及其形成方法和具有该反熔丝的非易失性存储器件的单位单元串联用电式信号处理电路及电子装置发光二极管的封装结构及其封装方法电子封装结构高速光电组件及其芯片倒装结构双压电体并排推动的三摩擦力步进器与扫描探针显微镜发光二极管及其制作方法、发光二极管的底座的制作方法三或四压电体并行推进的步进器及其扫描探针显微镜镜体硅衬底及其制造方法半导体装置与分压电路具多晶硅层及微晶硅层的双底材主动层结构、方法及装置硅晶片的受控边缘厚度一种半导体横向器件和高压器件具有垂直沟道晶体管的半导体器件一种记忆体阵列及其用于制造一记忆体阵列的方法只读内存单元阵列结构有源矩阵基板和显示装置高耐压半导体集成电路装置、电介质分离型半导体装置图像感测装置透镜模块及其制造方法固态成像装置和照相机用于沟道隔离的斜角注入有机电致发光显示装置有机发光显示装置双极晶体管的表面钝化结构双触发型可控硅整流器异质结场效应晶体管金属氧化物半导体晶体管自对准沟槽累加模式场效应晶体管结构薄膜晶体管及显示器件薄膜晶体管无铅二极管低频、低噪音、低闪烁的二极管用于薄膜太阳电池的陷光结构透明遮阳太阳能电池片四元半导体的异质结热光伏电池硅基纳米柱阵列异质结薄膜太阳能电池氮化镓太阳能同位素复合型微电池光学传感器半导体受光元件成像探测器透明导电氧化物涂层硅基高效双结太阳能电池薄膜太阳能电池异形LED器件

Engineering geological structure classification is divided into macro-, fine-, and microstructure. Macro-structures include alluvium predominated structure, multiple-binary-rhythm structure, thick boulder and gravel structure, saccate buried valley structure and tremendous composite structure, fine structures homogeneous structure, stuffed structure, porphyro-structure, and space frame structure, microstructures cemented, semi-cemented and loose texture.

利用第四纪构造与气候环境演变控制论的观点,在成因分类的基础上,对覆盖层进行了结构工程分类,按沉积相的不同组合特点分为冲积为主的结构、多重二元韵律结构、厚层漂卵石层结构、囊状槽谷结构、巨厚复合加积结构等五种类型;按颗粒组成和结合状态,分为均匀型、充填接触型、斑状型和架空型四种细观结构;按胶结成岩状态分为胶结、半胶结和松散三种微观结构。

(1) cerebral ischemical reperfusion injury rats'limbs motor function is variable. Acupuncture could promote lims'functional recovery.(2) PCNA masc cells is visible in cerebral ischemical semidarkness region. There is cell regeneration phenomenon. Acupuncture could strengthen injury region's PCNA expression, could profit injury recovery and functional reconstruction.(3) In ischemia semidarkness region for the model group and acupuncture group, PCNA masc cells percentage of 14days group is lower than 7days group. Along with the recovery of injury, cell multiplication is weaken.(4) In cerebral ischemia semidarkness region, there is VEGF masc cells and regeneration phenomenon. Acupuncture could strengthen injury region's VEGF expression, could profit protection after injury and blood vessel regenerate.(5) In ischemia semidarkness region for the model group and acupuncture group, VEGF masc cells percentage of 14days group is lower than 7days group. Along with the recovery of semidarkness region, ischemia and anoxemia state is getting improved, and VEGF is reduce.(6) As there are PCNA and VEGF masc cells in brain injured region, we could conclude that, after brain ischemical reperfusion injury, there are blood vessel regeneration phenomenon. Acupuncture could promote blood vessel regeneration, recovery blood supply sufficiently and quickly, and promote the recovery of brain injury region.(7)The VEGF masc cells percentage of inhibitor group is lower than acupuncture group. It state that the effect of acupuncture promote VEGF is partly depend on the existing of eNOS.

实验结论:(1)脑缺血再灌注损伤后大鼠的肢体运动功能发生改变,针刺可以促进肢体功能恢复;(2)脑缺损伤区可见PCNA阳性细胞,存在细胞再生现象,针刺可以增强损伤区PCNA的表达,有利于损伤的修复和功能重建;(3)针刺组和模型组14d时缺血损伤区PCNA阳性细胞百分比低于7d组,随着损伤逐渐得到修复,细胞增殖现象减弱;(4)脑缺血损伤区可见VEGF阳性细胞,存在内皮型细胞再生现象,针刺可以增强损伤区VEGF的表达,有利于脑损伤后保护和缺血区血管再生;(5)针刺组和模型组14d时缺血损伤区VEGF阳性细胞百分比低于7d组,随着缺血损伤的修复,缺血缺氧状态得到改善,产生的VEGF减少;(6)由于脑损伤区同时出现PCNA阳性细胞和VEGF阳性细胞,前者是增殖细胞的标志,后者是促进血管再生的重要因子,可以推断,脑缺血再灌注损伤后脑内存在血管再生现象,针刺可以促进损伤区的血管再生,更迅速而充分的恢复损伤区的血供,促进脑损伤区的修复;(7)抑制剂针刺组脑损伤区VEGF阳性细胞百分比与针刺组相比有不同程度的降低,说明针刺促进缺血损伤区VEGF表达部分依赖eNOS的存在。

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