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This study is to further investigate the mechanisms of osteoclastic bone resorption induced by IL-1 with established osteoclasts culture and osteoclasts, bone marrow stromal cells co-culture system.

本文利用建立的破骨细胞培养和破骨细胞、骨髓基质细胞共培养体系，在体外进一步研究IL-1诱导破骨细胞性骨吸收的机制，旨在探讨细胞因子在骨质疏松症骨丢失发生中的作用。

Methods Osteogenetic culture medium containing different concentrations of atorvastatin 10^(-6, 10^(-7), 10^(-8)mol/L and control were added in the murine bone marrow cell subculture. The proliferation of the stromal cells was observed, and alkaline phosphatase staining and examination were done to check the differentiation ability of bone marrow cells. Alizarin red staining was performed to evaluate the mineralization of the osteoblasts in culture.

分别将10^(-8)、10^(-7)、10^(-6)mol/L的阿托伐他汀和空白药物加入传代培养的小鼠骨髓基质细胞，通过细胞形态学观察、细胞增殖率检测、碱性磷酸酶检测和钙结节染色，比较药物在成骨细胞分化过程中对细胞增殖和分化的影响。

Simvastatin leads to the high expression of cbfa1 in bone marrow stromal cells and increased ALP activity, which may be parts of the mechanisms underlying the anabolic osteogenetic effect of simvastatin.

本研究采用体内给药然后体外对骨髓基质细胞培养诱导的方法，旨在观察辛伐他汀对骨髓基质细胞成骨分化的影响，以探讨其促进骨形成，治疗骨质疏松的机制。

Objective: Bone marrow stromal cells are commonly used in bone tissue engineering as osteogenic stem cells.

目的：骨髓基质细胞是最为常用的骨组织工程种子细胞。

Objective To study the isolation, culture, identification and osteogenic differentiation potential of mouse adipose derived stromal cells.

目的 研究小鼠脂肪来源基质细胞的分离培养、鉴定及向成骨方向分化的潜能。

This experiment is sought to study the contribution of different gene in osteogenous differentiation of bone marrow stromal cells and optimize the seed cells of bone tissue engineering.

为了优化骨组织工程种子细胞，探讨不同基因对骨髓基质干细胞成骨活性的影响，我们首先利用RT-PCR方法获得了全长BM P-2，VEGF 165及bFGF基因，克隆入真核表达载体pcDNA 3.0，鉴定正确后，经脂质体介导转入分离培养的大鼠骨髓基质干细胞，G 418筛选出稳定表达株。

Objective To investigate the biological effects of dynamic strain on the growth and proliferation of human marrow stromal cells and periosteal cells in vitro.

目的 探讨动态力学信号对体外分离培养的人骨髓基质细胞、骨膜细胞生长与分化特征的生物学效应。

Objective To investigate the proliferative and osteogenic activity of periosteal osteoblasts, calvarial osteoblasts and bone marrow stromal cells from the same embryo.

目的 比较从同一胚胎来源的骨膜成骨细胞、颅骨成骨细胞和骨髓基质干细胞的分裂增殖能力和细胞的功能状态。

Result: 1. The situation of HIV infection in gastro-mucosal tissues:(1) Our study found HIV gag sequence in not only CD4~+ T cells but also mucosalepithelial cells, gland epithelial cells in lamina propria and spindle stromal cells;(2) HIV p24 was expressed in T cells, plasmocyte, and a few mucosa epithelial cells, gland epithelial cellls and dell epithelial cellls.

结果：1.HIV在胃黏膜中的感染状况：(1)HIV感染者胃黏膜内HIVgag区基因不仅见于CD4~+T细胞等免疫细胞中，少数胃黏膜上皮、腺上皮、小凹内上皮细胞和间质梭形细胞中亦有阳性杂交信号；(2)HIV感染者尸检胃黏膜内HIVp24蛋白于T细胞、浆细胞及少部分胃黏膜黏膜上皮、腺上皮及小凹上皮细胞中呈阳性表达。2。

A challenge in such a use is expansion and directed seeding in itro, requiring proliferation and surial, and directed migration, respectiely, prior to functional differentiation. The epidermal growth factor receptor is the prototypal growth factor receptor and elicits these responses from a wide ariety of stromal, epithelial, and endothelial cells.

对于这一种细胞的应用所面临的挑战是在功能性分化前在体外的直接种植和扩增、增殖及分化以及导向性迁移等，表皮生长因子受体是一种原型生长因子受体可以在间质细胞、表皮细胞及内皮细胞中引出上述效应。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。