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①The collected clinical Acinetobacter baumannii isolates were more seriously resistable to Ciprofloxacin than to Imipenem;②The antibiotic resistance of clinical Acinetobacter baumannii isolates may be associated with the decline of CarO protein expression;③There is some relationship between CarO protein and ciprofloxacin-resistant Acinetobacter baumannii strains,it proves that CarO protein supports bacteria on multi-drug resistance;④CarO protein may be related to cell signalling;⑤The difference in CarO protein functional sites and structure between the sensitive strains and the resistant strains may be related to bacterial resistance.

①收集的临床鲍曼不动杆菌对环丙沙星的耐药情况较亚胺培南严重；②CarO蛋白的表达下调可能与临床鲍曼不动杆菌的耐药性相关；③CarO蛋白与鲍曼不动杆菌耐环丙沙星有一定相关性，佐证了CarO蛋白可能与细菌多重耐药有关；④CarO蛋白可能与细胞信号传导有关；⑤敏感菌株与耐药菌株的CarO蛋白功能位点和内部结构有差异，可能与细菌耐药有关。

102 phenotypic features of 16 strains isolated from Sesbania ,growing in dry hot valley region of panzhihua,were analyzed.13 reference strains were included for comparison.The result showed that there were great diversities among strains in carbon and nitrogen nutrient utilization,resistance to antibiotics,endurance to salt.

选用分离自攀枝花干热河谷地区的田菁根瘤菌未知菌16株和13株参比菌株，进行了102项表型性状测定，结果表明：供试根瘤菌株在碳氮源利用、抗生素敏感性、抗逆性等方面存在着差异。

Among the bacterial strains, 5 strains of RP,RS,RT,RX and RY were suscept ib le to all 20 phage strains, so they could be used as indicative bacteria for det ecting the phage particles in checking samples in order to forecast bacterial le af s treak of rice with the bacteriophage isolation technique in Hubei Province.

根据它们相互的亲和关系，可把10个细条病菌菌株划分为6个组，把20个噬菌体划分为7个溶菌型；其中RP、RS 、RT、RX、RY等5个细条病菌菌株可与所有参试的20个噬菌体发生亲和反应，据此在用噬菌体技术进行湖北省水稻细菌性条斑病的测报工作中，这些广亲和性的菌株可作为指示菌。

There was no significant difference between the fatty acid compositions of the toxigenic and non-toxigenic strains. Conclusion:FAME method is useful in rapid identification of Vibrionaceae strains and in field isolation of V. cholerae. It can be used to judge the relationships of strains in small outbreaks, but it cannot differentiate the subgroups of V. cholerae.

结论脂肪酸分型对弧菌种的快速鉴定有应用价值，对霍乱弧菌的现场分离鉴定有辅助意义，在小样本暴发资料的研究中能够反映菌株之间的亲缘关系，但其对霍乱弧菌种属内的各种特征性菌群不具有鉴别能力。

The specific band EM433 was recovered., cloned and sequenced, and 2 primers of Sequence characterized amplified regions markers, 5 --CGAGACGTCCGAGTC GATATAGC-3 - and 5 --GTCGTCCTTCTAGTGGGTATCCTCC-3 -, were deviced and systhesised in terms of the result of sequence. 41 strains of V dahliae were amplified specifically, 9 nondefoliating strains and 2 defoliating strains could yield EM.

将特异片段EM_(433)进行回收、克隆、测序，在此基础上设计并合成两条SCAR标记引物5&-CGAGACGTCCGAGTCGATATAGC-3&和5&-GTCGTCCTTCTAGTGGGTATCCTCC-3&，对41个V.dahliae进行扩增，10个非落叶型菌系中有9个菌系可以扩增出特异片段，31个落叶型菌系中29个菌系无此特异片段，鉴定准确率可达90％以上。

Among them, 25 strains with deodorizing ability, including 20 bacterial strains, and 5 fungus strains were screened.

将上述分离菌株接种到鸡粪培养基中，用臭味测评法对各菌株(以不接种的自然发酵作对照)进行初筛，获得25株细菌20株，真菌

Albicans strains with fluconazole. Method: A series of 16 strains C. albicans from the same body were induced to form pseudo-hyphae in RPMI 1640 medium and DMEM medium at 37 癈 for twenty-four hours respectively. The pseudo-hyphae and yeast were counted and the ratio between pseudo-hypha and total cells was calculated. C. albicans strains were induced to form pseudo-hyphae in RPMI 1640 medium for seven days by transferring twelve times. The genome DNAs of the hyphal-form of C. albicans was cleaved by two restriction enzymes EcoR I and Bgl II, the products were electrophored and photoed.

以同一母体来源的白念珠菌CA-1～A-17(共16株)作为研究对象，采用RPMI1640和DMEM培养基，37℃培养24h诱导其假菌丝形成，记录不同观察时间时的菌丝相和酵母相细胞数，计算菌丝相白念珠菌形成率；以RPMI1640培养基，37℃连续传代培养7天(转种12次)后，分离菌丝相白念珠菌并提取其基因组DNA；以EcoRⅠ和BglⅡ消化菌丝相基因组DNA，消化产物进行琼脂糖凝胶电泳并拍照；用随机引物(5'…TCACCACGGT…3')扩增菌丝相基因组DNA，采用变性聚丙烯酰胺凝胶电泳分离扩增产物并拍照。

Therefore, we suggest that pollination wasps could be introduced artificially to male syconia of phase B so as to prolong the phase D of male strains; and the match between female and male strains should be 1:2-4, namely, one female strain is cultivated with 2-4 male strains with successive D phases.

因此建议对雄株的B期花序进行人工引蜂的方法修复小蜂种群，从而延长雄性品系D期时长；而雌、雄品系配植模式应为1：2~4，即1个雌株品系与2~4个D期依次衔接的雄株品系配植。

Identification of extensive phenotypic information to 6 pare cultures and detection of the mol% G+C ratio of the DNA to representative strains, showed that the examined bacteria belonged to a new morphovar of Acinetobacter junii, and was designated as Acinetobacter junii Morphovar Ⅰ. In addition, serotype, antibiotic sensitivity and pathogenicity of isolates were studied, the results showed that the 6 strains have the same K-antigen and O-antigen, there arc no obvious differences in sensitivity and resistance to used 37 antimicrobial agents between strains, and have strong pathogenicity to experimental stone bonder and Bastard halibut.

经对6株纯培养菌在形态特征、理化特性等方面较系统的表观分类学指征鉴定及代表菌株DNA中G+C mol%的测定，表明为琼氏不动杆菌的一个新形态型并定名为琼氏不动杆菌形态型Ⅰ(Acinetobacter junii morphovar Ⅰ)；同时，对该菌进行了血清型、对抗菌类药物的敏感性及致病作用等方面的试验，初步表明此6株菌具有同种的表面及同种的菌体抗原，对供试37种抗菌类药物在不同菌株间的敏感及耐药无明显差异，对供试石鲽及牙鲆均具有较强的致病作用。

Cerevisiae have found that bZIP proteins play a role in yeast anti-oxidation. at3g51960, the transcription factor found in plants, is highly homologous to that of yeast. To examine the in vivo role of at3g51960, the overexpression and silencing vectors were constructed respectively. By using flower dip-dye, the binary expression vectors, pCAMBTA3301: at3g51960 and pCAMBTA3301: at3g51960Promoter, as well as vectors pCAMBTA3301: at3g51960-GFP and pCAMBTA3301: at3g51960cDNA were transformed to Arabidopsis by Tumefaciens. The transgenetic homozygous Arabidopsis strains have been derived after screening. Analysis on these strains reveals that strains overexpressing at3g51960 gene have higher level of stress resistance, implying a role of at3g51960 in plant stress regulation.

酵母中发现了bZIP家族的转录因子与酵母的抗氧化性相关，植物中的转录因子at3g51960与酵母中的这一转录因子在空间结构上有很高的同源性，为了验证它在体内的功能，本实验构建了过量表达载体和沉默载体，利用花浸染法把农杆菌携带的双元表达载体，pCAMBTA3301： at3g51960，pCAMBTA3301： at3g51960 Promoter和pCAMBTA3301： at3g51960-GFP及pCAMBTA3301： at3g51960 cDNA转入拟南芥，通过多次筛选已经获得了转基因拟南芥的纯合植株，对获得的转基因植株的表型分析发现，在过量表达at3g51960的转基因拟南芥中对胁迫的抗性有一定程度的变化，因此推测at3g51960可能参与植物的抗性调节。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。