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Different from the importation characteristic before 90s, strains after 90s were highly isogenous, suggested that the cholera epidemics in Sichuan province after 90s were endemic source. Inaba 1d strains from national wide epidemic in 2001 and 2002 were the same RT. Strains typed as different phagetypes (phagetype 6, phagetype 13 and phagetype 1) might be from a same clone.

除82-007、95-001外，所试菌株均具有致病性；20世纪90年代以前四川省发生的霍乱为输入性，90年代后四川省流行的菌株高度同源，具有地方疫源性；2001、2002年在四川省乃至全国引起大流行的稻叶1d为同一RT型，为传入性霍乱；噬菌体分型6型、13型菌株和1型菌株可能来自同一克隆系。

T standard strains and one fermentative strain MZ1 as templates, PCR results showed that 16 strains, which may be lysogenic strains, produced the product with the size about 750bp as anticipated.

t标准菌株和生产菌株MZ1（血清型H3a3h）的过夜培养物作模板，结果有16株菌株包括MZ1能扩增出大小约为750bp的预期产物，推测这16株菌为溶原菌。

According to one conserved sequences of phage GIL01 from Bacillus thuringiensis,a pair of primers was designed.With overnight cultivations of 35 B.t standard strains and one fermentative strain MZ1 as templates,PCR results showed that 16 strains,which may be lysogenic strains,produced the product with the size about 750 bp as anticipated.

根据苏云金杆菌(Bacillus thuringiensis， B.t)溶原性噬菌体GIL01(GenBank Accession Number： AJ536703)的同源序列设计一对引物，用35株B.t标准菌株和生产菌株MZ1(血清型H3a3b)的过夜培养物作模板，结果有16株菌株包括MZ1能扩增出大小约为750 bp的预期产物，推测这16株菌为溶原菌。

Analysis and fitting of growth curves in Liyang chicken;2. The results of antibacterial spectra and growth curves of eleven strains with better antagonistic effect showed that: 4 strains isolated from NAC plate had wider range of antibacterial spectra,3 strains isolated from manganic nutrient agar plate had advantage on growth.

用点种法从大黄鱼肠道优势菌群中筛选出104株病原性副溶血弧菌的拮抗菌，进一步研究拮抗效果最好的11株拮抗菌的抗菌谱和生长曲线，其中NAC平板上筛选的4株拮抗菌的抗菌范围较广，锰营养琼脂培养基上筛选的3株拮抗菌具有生长优势。

The strains are arranged in order in terms of biological characteristics as follows: B_ 、B_5、 F-263、 B_1、 B_6、 B_f、 B_(y2)、 B_(12)、B_z、 B_9、 B_3、 B_(14)、 B_2、 B_4、 B_8、 B_(13)、 B_(y7)、 B_(11)、 B_(10)、 B_7、 B_(15); through comparing the mortality and muscardine rate to Monochamus alternatus larva of 21 strains, it is found that B_5、B_ and F-263 are superior to other strains.

比较生物学性状，结果从优到劣排序是：B_、B_5、F-263、B_1、B_6、B_f、B_(y2)、B_(12)、B_z、B_9、B_3、B_(14)、B_2、B_4、B_8、B_(13)、B_(y7)、B_(11)、B_(10)、B_7、B_(15)；比较各菌株对天牛幼虫的致死率和白僵率，B_5、B_和F-263三个菌株优于其它菌株。

From 94 soil samples and 9 bark samples in these areas, 259 Myxobacteria strains were isolated and 140 strains of them were purified. These 259 Myxobacteria strains were primarily identified in genus or species level by their fruiting bodies, myxospores and vegetative cells on the base of Sergey's manual of determinative bacteriology and they belonged to 9 genera(Myxococcus, Corallococcm. Angiococcus. Archangium, Cystobacter. Stigmatella . Hapoangium. Nannocystis. Polyangium) of Myxococcales.

根据菌株的特征性子实体形态和其营养细胞、粘孢子形态，以《Bergey's manualof determinative bacteriology》第九版为依据，将分离纯化出的菌株初步鉴定到属，这259株粘细菌菌株经过初步的分类鉴定，分属于9个属，即粘球菌属139株、珊瑚球菌属20株、囊球菌属18株、原囊菌属21株、孢囊杆菌属5株、标桩菌属36株、单囊菌属9株、小囊菌属3株，和多囊菌属8株。

Of 20 strains of Klebsiella producing AmpC but nonproducing ESBLs, 7 strains were detected blaDHA gene and 1 strain with blaMIR gene; blaTEMgenes were amplified in 20 strains (100%), blaCTX?

肺炎克雷伯菌产AmpC酶但不产ESBLs的20株菌中7株检出blaDHA基因，占35%，1株检出blaMIR基因，占5%。

Comparing the nucleotide sequences of 16S rRNA gene and ribosomal protein gene, we could find that these two strains have the highest homology of 99.4% and 99.8%, respectively. The nucleotide sequences of 16S rRNA gene for the CWB-YN strain shared higher homology (98.5%,98.9% and 98.0%) with phytoplasma strains in Tomato big bug 16SrI-A group, Western aster yellow (16SrI-B group and Clover phyllody (16SrI-C group). But it is obviously under 97.0% with other phytoplasma groups. The CWB-GZh strain shared higher homology (98.7%,99.1% and 98.3%) with phytoplasma strains in Tomato big bug(16SrI-A group ),Western aster yellow (16SrI-B group) and Clover phyllody (16SrI-C group).

苦楝丛枝病植原体云南株系与其它组16S rRNA基因序列的同源率进行比较，结果与16SrI-A组中的番茄巨芽病植原体(Tomato big bug，BB)、16SrI-B组中的西方翠菊黄化病植原体(Western aster yellow，SAY)和16SrI-C组中的三叶草变病植原体(Clover phyllody，CPh)同源率达最高，分别为98.5%、98.9%和98.0%，而与其它组的植原体16S rRNA基因序列的同源率均低于95%；苦楝丛枝病植原体贵州株系与16Sr I-A组的番茄巨芽病植原体(Tomato big bug，BB)、16Sr I-B组的西方翠菊黄化病植原体(Western aster yellow， SAY)和16SrI-C组中的三叶草变叶病植原体(Clover phyllody，CPh)的同源率达最高，分别为98.7%、99.1%和98.3%。

In high dentity fermentation, the cellular respiration, energy metabolism, highest bacterial density and dry bacteria weight of the G830 strain were markedly better than control strains PA1 and BL21, The expression of recombinant prolyl endopeptidase in G830 and PA1 under the above condition was high and stable. Their growth situation and fermentation parameters were similar with their parental strains without plasmids, and resided plasmids maintained stably in those strains.

在高密度发酵条件下，G830的细胞呼吸强度、能量代谢、最高菌密度和细胞干重，均明显优于对照菌株PA1和BL21；重组蛋白脯氨醯内肽酶在G830和PA1中获得稳定高表达；重组菌生长状况及发酵指标均与空宿主菌基本一致且表达质粒能维持较好的稳定性。

Antibiotic susceptibility tests were carried out by K-B diffusion method on 41 vibrio strains isolated for one year both from farming seawater (18 strains) and digestion guts (23 strains) of the cobia, Rachycentron canadum . We chose 10 different species of antibiotics and refered to NCCLS performance standards for antimicrobial susceptibility testing, while taking Staphylococcus aureus ATCC25923 and Esсheriсhia coli ATCC25922 to control the test.

选用常用的10种抗生素，通过纸片扩散法（Kirby-Bauer纸片扩散法，简称K-B法），参照NCCLS抗生素敏感试验操作标准，并以金黄色葡萄球菌 Staphylococcus aureus ATCC25923和大肠埃希菌Esсheriсhia coli ATCC25922为质控菌株，对周年监测分离自养殖军曹鱼水体及肠道的41株优势弧菌（水体菌18株，肠道菌23株）进行了药物敏感性研究。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。