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The homology of the nucleotide sequences and genetic variation analysis of NDV strains suggested that mere were obvious divergence in gene lever among NDV vaccine strains and epizootic strains isolated from different areas and different ages.The divergence may have effect on amino acid and structure and function of protein in some extent and lead to difference of immunogenicity ,which was one of reason being difficult in preventing from ND.

通过对NDV F基因核苷酸序列同源性比较及遗传变异分析发现：我国不同年代、不同地区分离的NDV毒株与疫苗毒株之间在基因水平上存在较大差异，这种差异对氨基酸及蛋白质的结构和功能可能会有一定影响，而NDVF蛋白的差异同时也造成免疫原性的差异，这很可能是新城疫的免疫防治十分困难的原因之一。

A 1.2 kb fragment of the gyrB gene of 17 Gordonia strains including type strains were determined by direct sequencing of PCR amplified fragments. Total 25 strains (8 were retrieved from public database) of genus Gordonia form a distinct phyletic line in the gyrB-based tree and are separated from other closely-related species of genera to suborder Corynebacterineae.

在Gordonia菌种间之差异研究中则发现12种Gordonia gyrB基因序列相似度介於79.3%-97.2%，16S rDNA的序列相似度为96.2%-99.7%，可知Gordonia放线菌的16S rDNA保留性极高，较难利用此基因序列进行菌种间之区分。gyrB基因之变异程度无论是在菌属，菌种甚至各分离株间均较16S rRNA基因大。

It is the fact that three methionines distributed in the Hsp16.3 will begradually oxidated, in vitro, by hydrogen peroxide(H2O2). When the hsp16. 3 gene was cloned with pMV261 plasmid vector and transfected into Mycobacterium smegmatis, we found that the ability of anti-H2O2 will be decreased greatly among the strains of M. smeg. containing pMV261-hspl6. 3 while compared with the control strains of M. smeg. containing free plasmid vector. But we found that the ability of anti-H2O2 will be increased greatly among the strains of M. smeg. containing pMV261-38kDa while compared with the same control.

尽管Hsp16.3中有三个甲硫氨酸在体外能被过氧化氢(H_2O_2)逐一氧化，我们将Hsp16.3基因克隆到pMV261质粒上，并转染到耻垢分枝杆菌中发现，转染了pMV261-16.3的耻垢分枝杆菌抗过氧化氢的能力大大下降，而对照含pMV261-38kDa的耻垢分枝杆菌抗过氧化氢的能力却有明显的增强，据此，我们认为，Hsp16.3在细胞内可能不具有抗过氧化氢的能力。

Ceftazidime shows similar MIC on mucoid or nonmucoid strains in routine microbroth dilution test. In nonmucoid strains,both planktonic and sessile bacteria are sensitive to ceftazidime and 4μg/ml azithromycin shows little effect on the MBCs of ceftazidime while in mucoid strains, sessile bacteria in biofilms show high MBCs of ceftazidime and adding 4μg/ml azithromycin brings obvious reduction in MBCs of ceftazidime and enhance the sessile bacterial sensitivity to ceftazidime.

在非粘液型菌株，悬浮菌和BF菌均对头孢他啶敏感，加入4μg/ml阿剂毒素对头孢他啶的MBC影响较小；在粘液型菌株，头孢他啶对BF中的座生菌MBC增高，而加入4μg/ml阿剂毒素明显降低了头孢他啶的MBC，显示阿剂毒素增强了座生菌对头孢他啶的敏感性。

Results Isolated from 500 pneumonic patients were 334 strains of bacteria,of which 73 strains were isolated from patients with the community acquired pneumonia and 265 strains were isolated from pateints with ...

结果500例患者共分离出细菌334株，其中社区获得性肺炎患者分离菌株73株；医院获得性肺炎患者分离菌株265株。

Firstly,the result of the morphological character shows that all strains are short bacillus and gram-negative bacteria except DL7 strain;40%strains have gemma,and the strains which isolated from the root nodule of Podocarpus macrophyllus Thunb.

首先从形态特征的结果来看，所有菌株的菌体均为短杆状，革兰氏染色仅DL7菌株是阳性，其余菌株是阴性，有40%的菌株具有芽孢，其中从罗汉松根瘤分离的菌株形态与根瘤菌的有更多的相似性。

The results showed that the Saccharopolyspora spinosa SP06081 protoplast yield was the highest under these conditions: the collected mycelium from SP06081 grown in Tryptic Soy Broth medium with 0.2% glycin for 48 h was treated by 0.1 mg/mL lysozyme at 28oC for 20 min, then plated on the R2YE medium with sucrose as osmotic stabilizer, the number of regeneration protoplast was up to 108/mL. The protoplast-regenerated strains exhibited changes in morphology and antibiotic production, 29.3% protoplast-regenerated strains was characterized by loose mycelium and abundant broken branches as did their parent. Among them, 58.2% strains presented the trend to positive variation in spinosad yield, with the highest spinosad yield of up to 582.0 mg/L, 85.6% higher than that of their parent.

结果表明：菌体在添加0.2%的甘氨酸的TSB培养基中培养48 h收集， 0.1 mg/mL 溶菌酶， 28oC作用20 min制备原生质体，将原生质体涂布于以蔗糖为渗透压稳定剂的R2YE培养基中，原生质体再生数目最多，达108个/mL以上；原生质体再生菌株在形态和抗生素产量上产生分化， 29.3%的再生菌株形态上保持与亲本菌株一致，具有菌丝松散，断裂分枝多的特点，其中53.2%的再生菌株多杀菌素产量变异向正方向移动，最高产量达到582.0 mg/L，比亲本菌株提高85.6%。

Results The distinctive PCR products of the 6 isolated strains from Liaoning and Jilin areas through restriction with Hinf I ,Hha I ,Rsa I appeared two different kinds of RFLP restriction map. The strains HCAa9202,HCAp9203 and HCM9501 had the similar restriction maps with reference strain Gilliam,but they did not have restriction site of Hinf I ;Strains KDCt9402,KDCp9403 and KDCt9404 had the simular testriction map with reference strain Karp.

结果辽宁、吉林地区6株分离株的PGR特异性产物经Hinf I、Hha I、Rsa I酶切后呈现2种不同的RFLP图谱：HCAa9202株、HCAp9203株及HCM9501株与Gilliam参考株具有相似的酶切图谱，但缺乏Hinf I的酶切位点；KDCt9402株、KDCp9403株及KDCt9404株与Karp参考株具有相同的酶切图谱。

In this study, the cDNA fragments of G-L intergenic region and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae.

本实验对狂犬病病毒野毒株MRV、DRV的G-L间隔区以及L基因聚合酶活性部位进行了克隆和测序，将所测定的结果和推定的氨基酸序列与国内外公开发表的狂犬病病毒其它固定毒株、野毒株和同科不同属的病毒的相应部分进行了分析比较，结果表明MRV、DRV G-L IGR核苷酸序列的同源性为83.3％，与其它毒株的同源性在58.3％～91.7％之间；狂犬病毒G-L间隔区序列的长度在弹状病毒科不同病毒属之间有所不同。

Results: The 1034 strains of 8 types of pathogenetic aeromonase are sensitive to most of antibiotic and the highest resistance rate of 91.9% is ampicillin. The second one of 77.9% is piperacitlin and terramycin and sulfona mides are also with the high resistance rate of 62.4% and 56.9%. 127 strains (12.3%) which can produce the Ampc enzyme was found and 45 strains (4.4%) was multiple-drug resistance.

结果：8种1034株致病性气单胞菌对大多抗生素敏感，耐药率最高的是氨苄西林抗生素，达91.9%，哌拉西林次之，为77.9%，土霉素和磺胺类也有较高的耐药率，分别为62.4%和56.9%；检出具有产AmpC酶能力的致病性气单胞菌127株，占12.3%，多重耐药性菌株45株，占4.4%。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了