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In this experiment, the parent strains of Pleurotus ostreatus were 9400 and 803 .143 new crossing progenies strains were abtained by monospore cross breeding, and those strains were used to attain Pleurotus ostreatus strains with properties of good quality by a series of selecting experiments.

本试验以平菇9400和平菇803为亲本，通过单孢配对杂交，获得143个杂交子代菌株，并对其进行一系列的筛选试验，旨在获得平菇优良新菌株。

Six bacteria and five mildews have been separated from 119 strains, wliich grew well on the culture media contained lignin. The result of taxonomic identification is that XI, X2 and X3 belong to Pseudomonas, X4 and X5 belong to Xanihomonas, X6 belongs to Mycoplana. A preliminary study has been carried out on the capacity of lignin degradation of the 6strains of bacteria, and strains X2 and X4 were chose to carry out the experiments. The most favorable temperature for the 2 strains is 30癈, and the pH is 7; After being treated by ultraviolet rays, activity of all the three kinds of lignin-degrading enzyme produced by the strains can be increased greatly, the bacteria increasing rate is remarkably improved.

从119个菌株中分离筛选出了6株细菌和5株霉菌，它们可以在含有木质素的培养基上良好生长：细菌分类学鉴定结果为X1、X2和X3：假单胞菌属，X4和X5：黄单胞杆菌属，X6：枝动杆菌属；对6株细菌降解木质素性能进行了初步的研究，选定X2和X4为实验用菌种，两株菌适宜的生长温度为30℃，最适初始pH值为7；经紫外线照射诱变法处理，可使它们产生的三种木素分解酶活性均有明显的提高，细菌增长速率比诱变前明显提高。

objective to study the risk factors on esbl producing strains of k.pneumoniae and e.coli.methods aprospective survey on esbl producing strainsof k.pneumoniae and e.coli for a36-month period.results the resistance rates ofe.coli were:98.08%for ctx,73.08%for amc.the resistance rates of k.pneuˉmonialwere;95.74%for ctx,97.87%for amc.the use rate of third generation cephalosporins was much higher than those of esbls nonproducing strains（p.05）.it induce esbls that3rd generation cephalosporins were used extensive.after strenghtening the antibiotic controls,esbls detection rate has gone down.conclusion the factors of infection of esbl-producing strains were the severity diseases,cellular immunological condition,improper medical manipulations.to prevent esbl-producing strains,reasonable antibiotics usage may be the effective measure.

目的 分析临床大肠埃希菌及肺炎克雷伯菌产esbls的危险因素，并加以控制。方法前瞻性监测产esbls菌的情况，并对感染者进行临床调查。结果产esbls的大肠埃希菌、肺炎克雷伯菌的耐药率头孢噻肟钠为98.08%和95.74%，阿莫西林+棒酸为73.08%和97.87%，产esbls菌感染者头孢第三代的使用率（70.21%）显著高于非产esbls菌感染者（39.47%）（p.05）；第三代头孢的大量使用诱导esbls的产生。通过加强抗感染药物的使用管理esbls检出率开始下降。结论严重的基础病、高龄、机体免疫力低下，长期住院者是esbls菌感染的易感宿主，皮质激素、化疗及介入性疗法是esbls感染的高危因素。滥用抗感染药是产生esbls的重要因素，合理使用抗感染药是防止esbls产生的主要措施。

In this paper, 1554 strains of bacteria were isolated from the soybean rhizophere in the different continuous systems. Those biocontrol bacteria were screened in vitro and in vivo. 6 strains of them had the better control efficacy. They exhibited the different antagonism to the pathogens of soybean root in vitro. The strains 18BR2-2 and 18BR9-1 showed a wide range of antagonism against Fusarium oxysporum, F. avenaoeum, F. solani and Rhizoctonia solani in vitro. The 6 strains exhibited different control efficacy to soybean root rot in greenhouse and in field. In greenhouse, they could decrease the degree of soybean disease significantly, and the control efficacy of 18BR2-2 was the best and can reach 45.90%. In theory, soybean treated by 18BR2-2 may postpone the days of outbreaking of disease for 6 days. The strain 18BR2-2 also promoted the growth of soybean seedling and, raising the soybean height for 11.24%, increasing dry weight for 40.91% and fresh weight for 35.27%.

本研究从长期连作田种植的大豆及其根际土壤中分离细菌1554株，采用平板测试、温室盆栽及田间试验等方法，筛选了对大豆根腐病菌(主要是尖孢镰孢菌Fusarium oxysporum)有很好作用效果的生防细菌6株，在离体的条件下均表现出不同程度的拮抗作用，其中生防细菌18BR2-2和18BR9-1表现出很广的杀菌谱，对大豆根腐病菌尖孢镰孢菌(F.oxysporum)、燕麦镰孢菌(F.avenaoeum)、茄腐镰孢菌和立枯丝核菌都有很好的离体拮抗作用，并且这6株生防细菌在盆栽和田间的情况下均表现出对大豆根腐病一定的防治效果。

This paper compares variability of petroleum biodegradation bacteria in frozen and thawed soil collected from Liaohe Oil Field and their capacities for biodegrading petroleum were also determined. The results show that 14 strains of bacteria and 6 strains of fungi for petroleum biodegradation were screened from thawed soils, while only 5 strains bacteria and 2 strains fungi existed in freeze soils.

以辽河油田不同年代开采油井附近采取的土壤样品为研究对象，通过冻融模拟试验和采用选择性培养基筛选对石油污染土壤具有原位修复作用的优势微生物菌株，并比较油田冻结土壤与未冻结土壤中石油降解细菌与真菌的数量变化特征及其对石油的降解能力。

Amplified fragment length polymorphism was performed on 41 strains of V dahiiae of cotton in search of DNA probes specific for distinguishing between nondefoliating strains and defoliating strains. Specific bands of 433 base pairs and 1 lObp amplified only from nondefoliating strains were obtained using two pairs , E64 M53 and E49 M65 ,which named EM433 and EM110, respectively.

选用25对EcoRI和MseI引物组合，对供试的41个V.dahliae进行AFLP扩增，筛选到两对引物E64、M53和E49、M65，能分别扩增出433bp和110bp两条仅为V.dahliae非落叶型菌系独有的特异片段，可将落叶型与非落叶型菌系分开，这两条特异片段被命名为EM_(433)和EM_(110)。

During the first cross, 4 different white monokaryotic strains were hybridized with 14 kinds of yellow monokaryotic ones to obtain light-yellow strains, which were then used to fruiting experiment. In the second cross, five kinds of yellow monokaryotic strains, which were observed monokaryotic fruit body, and 12 types of white monokaryotic ones were crossed to acquire hybrid strains, which were also to the experiment of fruit body formation.

第一轮杂交，运用4个不同的白色单核菌株和14个不同黄色单核杂交，获得系列浅黄色杂合子菌株，用于出菇实验；第二轮杂交，运用5个能够获得单核子实体的不同黄色单核菌株与12个不同的白色单核菌株相互杂交获得杂合子菌株，用于出菇实验。

In litmus milk experiment, all of strains produced alkai expect for MZ02 and MZ04 produced acid reactions. At 30℃, all of strains grew best, and had a wide range in salt, acid and basic resistance. MZ04 was capable of good growth at salt density of 2.0%, MZ04 and MZ10 could still grew better at pH 9.0. All of the strains were not resistant to 250 μg /mL gentamicin(Sm3), and to other antibiotics, different resistances were presented by different strains, MZ02 had worst resistance to all antibiotics, while MZ04, MZ08,MZ09 had strong resistance.

所有供试菌株在温度30℃时生长最好，耐盐、耐酸碱范围较宽，MZ04在含盐浓度为2.0%环境还能生长良好，MZ04、MZ10在pH9.0环境仍能生长较好；所有菌株对250 μg /mL 庆大霉素（Sm3）均无抗性，不同菌株对其余供试抗菌素表现出不同的抗性，MZ02抗性最差，对所供试的抗生素均无抗性；MZ04、MZ08、MZ09抗性相对较强。

Results MRSA strains were isolated from the burn wounds in 22 of 41 (53.7%) patients, and 5 from the nasal vestibules. Moreover,among 19 medical staffs,MRSA strains were isolated from the hands of 9 persons, but not from the nasal vestibules. From the hands in 9 of 43 lay attendants and the nasal vestibules in 2 MRSA strains were found. Thirteen MRSA strains were isolated from 193 specimens from the surrounding items.

结果 53.7％(22/41)的患者创面分离出MRSA，其中5例鼻前庭分离出MRSA；19名工作人员中，3人手分离出MRSA，工作人员鼻前庭未分离到MRSA；43例患者陪护家属中有9人手上分离出MRSA，2人鼻前庭分离出MRSA；193份环境标本共分离MRSA 13株。

Methods The serotyping, hybridization and RAPD typing methods were used. Seven strains of GBS were detected in 2 pairs of mother-baby, which included 2 strains from the 2 mothers and 5 strains from their babies with early and late-onset infections. Eighty-eight strains of GBS were detected in vaginas of pregnant womens.

采用血清学、核酸杂交和PARD方法对从2例诊断为早发型GBS肺炎和晚发型GBS脑膜炎患儿体内分离的5株GBS和其母阴道分离的2株GBS菌株作母婴配对分析，对88株孕妇生殖道携带GBS进行RAPD分型研究。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了