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The microorganisms was domesticated by adding a certain amount of lead in the soil, and three lead-tolerant strains including Pb-R-1, Pb-R-2 and Pb-H-3 accordingly originating from 300 mg/L, 750 mg/L and 900 mg/L lead medium were isolated with a series of PDA medium of different lead concentrations. Through the identification, Pb-R-2 was confirmed as Aspergillus sp., Pb-R-3 as Penicillium sp. The trials of transferring culture showed that all strains had the stable heredity of lead tolerance in different composition PDA medium.

通过向土壤中添加一定量的铅来驯化土壤微生物，然后利用一系列不同铅浓度的PDA培养基分离耐铅微生物，结果获得一株耐300 mg/L铅的细菌Pb-R-1和二株分别耐750 mg/L和900 mg/L铅的真菌Pb-R-2和Pb-R-3，通过鉴定得知，Pb-R-2菌株为曲霉，Pb-R-3菌株为青霉；不同成分培养基传代培养实验表明，3个菌株有稳定的耐铅遗传性；通过液体悬浮培养获得：Pb-R-1、Pb-R-2和Pb-R-3对Pb(上标 2+)的吸附率依次为35.5%、75.5%和48.2%。

This developed a new material for the wheat virus-resistance breeding.In order to breed maize dwarf mosaic disease梤esistance maize species, the PAP gene was introduced into maize inbred strains and hybrid strains mediated by pollen and particle bombardment,10 transgenic maize plants were achieved and the PAP gene was indeed integrated into the maize genome by analysis of molecular biotechnological methods. The PAP gene in one of the transgenic maize plants had been transcription and translated, the ability of the virus resistance is undergoing.

为了解决玉米矮花叶病害的防治问题，采用花粉介导法及基因枪法将PAP基因分别导入玉米的杂交种及自交系，获得了10株转基因玉米，PCR及Southern-blot检测结果表明PAP基因已经整合到了玉米基因组中，并且其中有一株PAP基因已经得到了表达，病毒抗性鉴定表明转基因玉米能明显推迟玉米矮花叶病害症状的表现。

Methods Five hundred and four specimens were isolated from the wounds and nasal vestibules of burn patients ,the hands and nasal vestibules of medical staffs and lay attendants and the surfaces of various equipments. From these specimens,58 strains of MRSA and 43 methicillin- sensitive staphylococcus aureus were isolated. The genome DNA of isolated MRSA strains was analyzed by repetitive DNA - sequence- based PCR analysis.

方法采集烧伤患者的创面、鼻前庭，工作人员手、鼻前庭，陪护家属的手、鼻前庭及烧伤科病房各种环境表面共504份标本，从中分离到MRSA 58株，对苯唑西林敏感的金黄色葡萄球菌43株，并对所分离的MRSA菌株的基因组DNA进行重复序列PCR检测。

According to the physiochemical and microbacterial results of them, two strains No. RC212and DV10which presented powerful fermenting performance and good production conformability were selected as test strains for "Industrialized Production Techniques Research of Rosa Roxburghii Tratt Claret".

经比较筛选出RC212号葡萄汁酵母与DV10号汉氏德巴利氏酵母两菌株，其均表现出较强的发酵性能及生产适应性能，为&刺梨干红工业化生产技术研究&提供了试验菌株。

By the pot experiment in nitrogen-free sand, the difference of the parental strain and marked strains with cfp gene on three cultivars was tested. One marked strain which was not significantly different with the parental strain for the competition stady with soil indigenous strains under field conditions .

通过无菌砂培试验，进行了标记菌株与出发菌株在三个品种上的差异性检测，选出一株在瘤数、植株干重、叶绿素含、全氮含量等各方面指标与出发菌株最为接近的标记菌株用于控制条件下的田间小区试验，比较标记菌株与土著菌株在三个品种上的竞争结瘤能力，结果表明

To identify the strain, the 16S rDNA sequence was compared with type strains of the same genus. The result can be abtained according to the homology.9 strains of Lactic acid cocci were isolated from milk and silages.

将含有阳性重组子的菌体进行测序，所得结果分别与Genbank上发表的同属的代表菌株的16S rDNA序列比较，得到相似性百分比，则可判定菌株的归属。

Lentinula edodesPegler, the shiitake mushroom, is one of the most widely cultivated mushrooms worldwide. The purpose of the project is to investigate the characteristics of 48 isolates or cultivated strains and 10 foreign countries strains.

香菇是广为世界栽培的主要食药用菌之一，亦为台湾主要栽培的食药用菌，本研究收集目前台湾各地主要栽培香菇菌株48株及外国菌株10株。

Methods In addition to the biochemical,serotyping and antimicrobial susceptibility test for identification of typical strains,the new strains were tested also by the RiboprinterDNA fingerprint system.

方法在部分测试典型菌株用生化、血清型别及药敏试验等的基础上结合R iboprinter DNA指纹图谱分析系统来分析鉴定测试菌。

Results Eighty-eight strains of Pseudomonas aeruginosa were isolated from 3 ICU, 80 of which were serotypied, the serotyping rate was 90.91%. 52.27%, 22.73% and 9.09% was type B, C and untypied Strains respectively.

结果 3个ICU共检出铜绿假单胞菌88株，对其中80株进行了血清学分型，分型率90.91%；所占比率较大的血清型为：B型52.27%，G型22.73%，未分型菌9.09%。

The results obtained showed that the percentage of agreement between FA method and routine culture method was 95%, A total of 265 strains of Salmonella were isolated, of which 235 strains were serotyped and all belong to A-F groups.

试验中共分离到沙门氏菌265株，其中经过群型鉴定的235株，全部属A-F群血清型内，和我们以往多年所得的结果一致。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。