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Some 90% of the tested 110 extracts appeared antitumor activity and 13 strains of the 55 isolates showed higher inhibitory capacitieseffective concerntrations ≤16μg/ml）, which may imply a possiblility in their further medical application;whereas the percentage of positive extracts was higher from the thalli than that from the supernatants and of actived strains was higher in the bacteria than that in the molds according to this study.

结果表明，55株微生物发酵样品共110个提取物中，90％样品表现出细胞毒活性；其中13株微生物的活性较强（提取物有效抑制浓度≤16μg/ml），具有较好的开发应用前景。同时还发现，菌体中检测到的活性菌株数大于发酵液中检测到的活性菌株数，细菌的筛选得率高于霉菌的筛选得率。

To determine the regulating role of mtlR in transcription of mannitol PTS operon in V.cholerae,and whether it plays a role in the ferment difference of the toxigenic and non-toxigenic strains,the mtlR deletion mutants from the mannitol rapid-ferment strain(non-toxigenic strain) and slow-ferment strain were constructed.Comparisons of the growth in M9 containing 0.2% mannitol as the sole carbon source and pH change in mannitol fermentation media of these wild strains and their mutants,indicated that mtlR is a repressor.Its repression in mtlCBA transcription was further verified with the analyses of quantitative reverse-transcriptional PCR.

通过比较快发酵菌株和慢发酵菌株mtlR缺失突变株与野生株在含0.2%甘露醇的M9培养液及甘露醇发酵液中生长、产酸等的变化，定性地证明了mtlR基因的抑制作用；另外通过定量RT-PCR进一步验证了MtlR蛋白在mtlCBA转录水平发挥负调控作用。

By pairing culture, 12 strains had apparent antagonistic effect to Pythium ultimum, ca. 2.67% of all tested strains showed antagonism, whose radius of suppression cycles was between 3.1~16.4 mm.

室内平板对峙法获得对黄瓜猝倒病菌有明显拮抗作用的菌株21个，所占比例为2.67%，其抑菌圈直径为3.1~16.4 mm。

Methods: Unrepeated MRSA strains from clinical specimens were divided into two groups: C-MRSA and H-MRSA, We used Chelex 100 to get DNA template and amplified mecA gene by PCR. The DNA templates of MecA were used to RAPD(random amplified polymorphic DNA), then we got the RAPD-DNA fingerprint-maps, randomly sampled 20 strains from each group as study objects.

收集临床MRSA标本，将其分为社区获得性MRSA与医院获得性MRSA两组，用Chelex 100裂解法制备DNA模本，PCR扩增mecA基因，选择mecA基因的DNA模本，用RAPD技术扩增出各菌株的RAPD-DNA指纹图谱。

Coli. Methods Among 95 unrepeated strains isolated from clinic, 38 positive strains tested by ESBLs confirmatory test were collected from HAI and CAI subjected to detection for drug resistant gene TEM, SHV, CTX-M-1 and for disinfectant resistant gene qacE△l-sull by polymerase chain reaction.

收集从临床标本分离的大肠埃希菌95株，经ESBLs确证试验将其中38株阳性菌株分为医院与社区获得性感染2组，用聚合酶链反应检测耐药基因TEM、SHV、CTX-M-1以及耐消毒剂基因qacE△1-su l1。

Methods The vaccinal strains Z10and Z37of HFRS bivalent inactivated vaccine were adapted in vero cells and continuously porpagated. The Vero-cell derived HFRS purified vaccine were developed using the different passages of these 2 viral strains.

方法将HFRS双价沙鼠肾细胞灭活疫苗的生产疫苗株Z10、Z37适应到VerO细胞上进行连续传代，并用不同代次的VerO细胞适应株制备VerO传代细胞疫苗。

METHODS HP strains were obtained by culture from patients Vero cell intracytoplasmic vacuolization was induced by Broth Culture Filtrate ( BCF, 0.22um pore filter) of 43 of 62 HP strains obtained from patients.

62株临床分离的幽门螺杆菌，43株的肉汤培养滤液(0.22um微孔滤膜)作用于体外培养的Vero细胞，能使其产生胞浆空泡样变。

Results 336strains of pathogenic bacteria were separated from the urine sample of2865cases and the detectable rate was11.73%of the336separated strains the number of strains of gram negative bacilli was226,the gram positive cocci is92and the fungi is18.the positive rate of escherichia coli was the highest followed by co-agulase negative staphylococci,klebsiella pneumoniae and blue verditer pseudomonas.highest resistance to penicillins and cephalosporins was obsered.

结果 从2865份尿标本中分离出336株病原菌，检出率为11.73%。其中革兰阴性杆菌226株，革兰阳性球菌92株，真菌18株。阳性率最高的是大肠埃希菌，其次是凝固酶阴性葡萄球菌、克雷伯菌、铜绿假单胞菌。药敏结果对青霉素类、头孢类抗生素的耐药率较高。

Methods The fluconazole, amphotericin B and itraconazole susceptibility test were performed by Rosco disk diffusion method and NCCLS M27A broth macrodilution method with 110 yeastlike fungus strains isolated from clinical elderly patients specimens. 3 standard strains were used as the quality control.

应用 Rosco 纸片扩散法和美国临床实验室标准化委员会M27A宏量肉汤稀释法测定临床分离的110株老年病例中不同酵母样真菌菌株对氟康唑、两性霉素B、伊曲康唑的药敏状况，并以3株酵母菌株作质量控制。

In this paper we study the biodegradation of petroleum wastewater by microorganisms with a new way.For the chain alkyl hydrocarbon in crude oil, strains were isolated by screening and acclimatizing at the existence of diesel oil and kerosene. For the aroma hydrocarbon in crude oil, strains were isolated by screening and acclimatizing at the existence of phenol.

本试验我们针对石油中的链烷烃，分别以柴油和煤油为代表物进行筛选得到以降解链烷烃为主的菌种：针对石油中的芳香烃，以苯酚为代表物进行筛选得到以降解芳香烃为主的菌种：针对石油中的环烷烃，以环己烷为代表物进行筛选得到以降解环己烷为主的菌种。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。