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The bacteriostatic effects, action mechanisms and antimicrobial spectrum of the strains were tested. After 5 days of culture, a bacteriostatic belt of 6.9-9.67 mm in width was observed on the culture plate, and the inhibitory activity maintained for 10 d. Prefiltration fermentation of the strains P1 and P5 could significantly inhibit conidial germination of the pathogen, with inhibitive rate ranging from 97.51% to 98.12%. The action might be realized by inducing hyphal deformity, bioplasm concentration, conidial germination inhibition and distorting germ tubes of conidia.

通过对其抑菌效果、机制和抗真菌谱进行测定,结果表明,P1、P5和葡萄灰霉病菌对峙培养5 d后,均产生明显的抑菌带,抑菌带宽度为6.90~9.67 mm,培养10 d后,仍然保持稳定的抑菌效果;P1、P5的发酵原液对葡萄灰霉病菌孢子萌发具有显著的抑制作用,抑制率为97.51%~98.12%;P1、P5对病菌的作用机制表现为使病菌菌丝畸形、原生质浓缩、菌丝断裂、抑制病菌分生孢子的萌发、导致孢子芽管扭曲。

In order to verify the results of numerical taxonomy, Besides the examined strains in numerical taxonomy, 6 strains isolated from Caragana sp were analyzed by SDS-PAGE of the whole-cell protein.

本论文简要回顾了生物的界级分类学说,综述了根瘤菌分类的历史和研究现状,描述了根瘤菌分类的方法。

In order to well understand the interaction between cementite and matrix, the plastic strains of two phases and the misfit of plastic strains between them were analyzed based on the Eshelby/Mori-Tanaka model.

为了更好地理解渗碳体相和铁素体相之间的相互作用,在Eshelby/Mori-Tanaka模型的基础上分析了两相各自的塑性应变及两相间的塑性应变失配。

The phytoplasmas diseases were investigated in 30 counties and cities of 10 areas in Yunnan Province. There are 12 phytoplasma diseases, including paulownia witches-broom,chinaberry witches-broom, cactus witches-broom, christmas cactus witches-broom, orange little leaf, petunia flat stem and so on were indentified using PCR with universal primers, which amplified phytoplasma 16SrRNA gene and/or ribosomal protein genes. So the kind of phytoplasma diseases in Yunnnan Province were understood. Identified the characteristic of 16SrRNA and rp gene of phytoplasma and the kind, classification and genitic relativity of these strains using RFLP, clonging and sequence analysis (nearly 20 full-length sequences of phytoplasma 16SrRNA and/or rp gene were submitted in GenBank ); The phytoplasma strains isolated from herbage plants were conserved in storeroom. 9 articles were published in the key journals of China and 1 technique invention patent was applied. The net-page about phytoplasma disease was established. 4 graduate student were bringed up.

对云南省10个地区的30余个县市地区进行了植原体病害的调查采集工作;采用植原体16SrRNA基因通用引物(R16mF2/R16mR1及R16F2/R16R2)和/或植原体核糖体蛋白基因引物(rpF1/R1)对所采集到的病害标样总DNA进行PCR扩增,根据植原体特异扩增条带的出现,共鉴定出泡桐丛枝、苦楝丛枝、仙人掌丛枝、蟹爪兰丛枝、柑桔小叶、矮牵牛扁茎等12种植原体病害,初步明确了云南省植原体病害的种类;通过RFLP技术、克隆及测序技术获得其16SrRNA及部分核糖体蛋白基因的近全长序列(已在GenBank中共登录近20余个相关序列),通过序列比较分析,明确了上述植原体株系中这两个基因的特征并确定了云南省植原体株系的种类、分类地位及遗传相关性;对一些发生在草本植物上的植原体进行了株系保存;在国内核心期刊及一般期刊发表研究论文 9 篇,申请技术发明专利1项;初步建立了有关植原体病害的网页;培养研究生4名。

The results showed that Hyphae, conidiophore, conidium and chlamydospore of biocontrol strains ZK7 and IPC were found in the rhizoplane, Hyphae of ZK7 and IPC penetrated epidermal cells to deep cortical cells but not in phloem tissue and xylem vessels, biocontrol strains ZK7 and IPC also crossed and even grew within epidermal and conical cell walls without any visible alterations of the plant cell.

生防菌ZK7和IPC菌株能以菌丝方式稳定定殖于烟草根表的根冠区、伸长区、根毛区、成熟区等不同部位,而在根内的定殖仅局限于根表皮层较深的细胞组织,不能侵入烟草根部的韧皮部和木质部组织。

Besides, we got new strains from environment, which might be advantage to study the synergies of different strains to increase the viability and the degradation rate of chlordane in high concentration.

同时,从环境中得到了更多的高效降解菌,为今后研究多种降解菌之间的协同作用,从而提高降解菌在实际环境体系中的生存能力以及对高浓度氯丹的降解能力打下良好基础。

Among them, 134 strains were gram-positive bacteria (75.71%), and the coagulase negative staphylococcus was dominant; 43 strains were gram-negative bacteria (24.29%), and the Klebsiella pneumoniae was the most frequently isolated.

革兰阴性菌43株,占24.92%,革兰阴性菌以肺炎克雷伯菌居多,占阴性菌的41.03%,占总分离率的9.04%。

Strains from farming water and intestine of cobia showed different sensitivity to the same antibiotic. 4 strains which resisted to 3 or more than 3 species antibiotics were sieved from this study.

不同来源(即分别来自养殖水体和鱼肠道)的弧菌菌株对同种抗生素的敏感情况存在一定程度的差异。

MRS and modified TJA culture medium were taken as the foundation culture medium, and 8 strains were isolated from natural fermented meat products in this paper. 4 strains were identified as lactic acid bacteria, among which, three are bacillus, one is coccus.

以MRS和改良TJA培养基为基础培养基,从自然发酵肉品中分离得到8个菌株,其中4株为乳酸菌,3株为杆菌,1株为球状菌。

About 18 Haemophilus paragallinarum strains were isolated and identified in different sites, there are 3 B serovar strains in them, indicating the new epidemiological characteristics of infectious coryza in China.

2001年至2003年通过从不同地区的免疫失败鸡场分离到18株副鸡嗜血杆菌的血清型鉴定,有3株为B血清型,说明我国鸡传染性鼻炎的流行已经出现了新的特点。

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