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Design another set of specific primers, of which upstream and downstream primers were located in the two sides of the cleavage site respectively, to develop the one-step RT-PCR method for detecting H5 and H9 subtypes of AIV. Identify the sensitivity of the developed one-step RT-PCR method by detecting 2 AIV strains of H5 subtype and 3 AIV strains of H9 subtype, and the specificity by detecting the RNAs of NDV, IBV and IBDV as well as analogue detection using computer software.

根据H5、H9亚型HA基因序列，分别设计一套特异性引物，其上、下游引物分别位於裂解位点两侧，应用RT-PCR一步法检测AIV H5和H9亚型，并验证所建立方法的敏感性；通过检测NDV、IBV和IBDV等RNA病毒及电脑软件模拟检测，验证其特异性。

Anise oil is a natural spice in Guangxi. Isolation of trans-anethole degrading bacterial strains and trans-anethole biotransformation process were performed using anise oil as the only carbon source. The target strains were expected to produce high value-added nature spice and medicine intermediate by biotransformation of trans-anethole—the main constituent of anise oil. Bacteria were isolated from soil under aniseed trees by enrichment culture.

以广西特色香料资源茴油为碳源，开展了反式茴脑生物降解菌株的筛选工作，并对反式茴脑生物转化过程进行了初步研究，以期获得的菌株能催化茴油中的主要成分反式茴脑，合成高附加值的天然香料或医药中间体。

Evaluation of IEM103 in rabbit animal model.The evaluation was based on the control-challenge study (IEM101 as control). Rabbits were laparotomized, injecteded 10〓 cfu directly into proximal small bowel, and 28 days later, challenged with 0.25--2ug CT and 10〓-10〓 cfu 4 wild toxic strains with different biotypes and serotypes. Experimental group developed antitoxic and significant vibriocidal immune responses to vaccination, and could protect no less than 2ug CT and 10〓-10〓 cfu all wild strains challenge.

IEM103的免疫原性评价：经兔肠段结扎免疫〓cfu IEM103，7天后在外周血中可检测到外周淋巴细胞分泌性抗CT IgG，血清中可检测到抗CT抗体、杀弧菌抗体，P／N均≥2，且可持续至28天。28天后用0.25-2ug CT毒素，四种毒株滨-43，吴江-2，395，1119〓cfu菌量攻毒，实验组可保护至少2ug CT攻毒，而对照组可保护1ug以下CT攻毒，两组均可保护〓cfu四种毒株攻毒（以肠积液量≥1.0ml／cm为不保护判断标准）。

According to recent researches, intracellular signalling pathway systems involved in appressorium differentiation is related with cAMP pathway and MAP pathway. In this research, 21 bacteria strains and Chaetomium cupreum strains were assayed for their antagonism to appressorium formation in M.grisea.

随着稻瘟病菌一系列致病基因的克隆及功能明确，附着胞形成的信号传导途径包括：cAMP途径和MAP途径，这些途径中基因的失活都影响附着胞的形成。

Main results were presented as follows:Among 21 bacteria strains, 3 strains induced conidia of the pathogen to produce swollen cells on the sites of the appressorium differentation.

本研究筛选到四类对稻瘟病菌附着胞形成具有不同抑制机制的拮抗细菌，并对h23菌株的抗菌活性进行详细分析；同时还测定了毛壳菌对稻瘟病菌附着胞的抑制作用。

Sixty single ascospore strains of Morchella angusticeps Boudier were crossed between each two single ascospore strains.

本文对自黑脉羊肚菌Morchella angusticepsBoudier分离到的60个单孢菌株进行了两两杂交实验。

This research has used the different origin Bacillus licheniformis strains, and the in-depth liquid fermentation production bacitracin and has studied the different strains, different carbon sources, different commensurability zinc and phosphorus to bacitracin high production effect.

采用细胞融合技术得到的菌株与不同来源地衣芽孢杆菌菌株，进行深层液体发酵生产杆菌肽，研究了不同菌株，不同碳源，不同量锌和磷量诸因素对杆菌肽高产的影响。

The bacteriostatic experiment showed that 13 strains had antifungi activity and 2 strains had resistant gram-positive bacteria.

抑菌实验表明，携带KS基因的16株放线菌中，13株具有抑制真菌活性，2株具有抑制革兰民阳性细菌活性。

In a commercial nursery using the peat moss-plug system, these three strains also showed their ability to promote seedling growth following either seed bacterization or peat moss drenching, and their populations in peat moss maintained at high levels (except strain RS65 for 30 min seed soaking ) within 21 days after seeding. The above 14 strains that were capable of promoting tomato growth were tested for their ability to control bacterial wilt of tomato.

自台湾中部不同地区数种作物分离得到之396株根栖细菌，被覆於番茄种子后，利用套袋塑胶培养皿之系统及含泥炭土栽培介质之穴盘系统於生长箱筛选，共有 14株菌株在重复筛选过程中均能提高种子发芽率及促进根生长之效果，其中RS4、RS65及RS70三菌株表现最佳，可增加根长度、茎长度、植株鲜重及乾重(RS65菌株除外)。

Certain species or strains have evolved such tolerance, and some, for example certain strains of bent grass (毛状剪股颖 Agrostis capillaris), benefit from the reduced competition in soil containing heavy metal ions.

有时某些种类或品系对重金属浓聚物得耐受性还能发展进化，例如某些剪股颖(毛状剪股颖 Agrostis capillaris)生长在含有重金属离子的土壤中，以此来降低竞争压力。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。