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Methods Germ tube test, chlamydospore test, CHROMagar Candida and API20 kit system were applied to separate non-Candida albicans strains from Candida albicans. Then PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 4 species of common Pathogenic non-Candida albicans (Candida glabrata, Candida parapsilosis, Candida krusei and Candida tropicalis), and the products were digested with the two restriction endonucleases (Msp Ⅰ and Hae Ⅲ) respectively. Results The four isolates consisted of 15 strains of Candida glabrata (7.50%), 7 strains of Candida parapsilosis (3.50%), 5 strains of Candida krusei (2.50%), 2 strains of Candida tropicalis (1.00%).

首先采用芽管试验、厚壁孢子试验、法国科玛嘉念珠菌显色培养基及API20CAUX酵母菌鉴定系统将分离自VVC患者阴道内的念珠菌菌株鉴定到种，然后采用真菌通用引物将4种常见非白念珠菌（包括光滑念珠菌、近平滑念珠菌、克柔念珠菌和热带念珠菌）进行PCR扩增，并选用MspⅠ和HaeⅢ两种内切酶对扩增产物进行酶切分析。

The high-risk strains of human papillomavirus are known to cause cervical cancer and releated to oropharynx cancers.

高危型人乳头瘤病毒（high-risk strains of human papillomavirus， HR-HPV）感染人类生殖器官和口腔上皮细胞，引起一系列恶性肿瘤。

Methods One hundred and fifty strains of Malassezia yeasts from pityriasis versicolor (29 strains), Malassezia folliculitis (16 strains), seborrheic dermatitis (49 strains), onychomycosis (33 strains), and healthy individuals(23 strains) were studied, according to physiological and morphological features in culture, and compared to standard strains.

方法以标准株作对照，用生理生化学及形态学方法将150株来源于花斑癣（29株）、马拉色菌毛囊炎（16株）、脂溢性皮炎（49株）、甲真菌病（33株）及正常人皮肤（23株）的马拉色菌进行分类及描述，并分析了各菌种在一些皮肤病的分布情况。

The cultural condition of PGPR strains Temperature,pH,light,cultural method,acid or alkali producing,and salt tolerance of 9 PGPR strains isolated from Gramineous Forage were tested,the results showed that all the PGPR strains could grow in the temperature range from 5℃～45℃,and optimum temperature for 178,O-6,Dry6 strains was 35℃,for X5,173,Y5,C-6 strains was 30℃,for N4 strain was 25℃～35℃,for 86 strain was 25℃;most of PGPR strains prefered to neutral or alkaline condition,strains 178,O-6,N4 and X5 were preferable to alkali condition especially;light was beneficial to PGPR's growth;all of them produce alkali;most of PGPR strains were not sensitive to NaCl concentration;all the strains were aerobiotic bacteria.

结果表明：各供试菌株对温度的适应范围较广，在5℃～45℃范围内均能生长，178、O-6、Dry6 菌株的适宜生长温度为35℃，X5、173、Y5、C-6 菌株的适宜生长温度为30℃，N4 菌株的适宜生长温度为25℃～35℃，86 菌株的适宜生长温度为25℃；大部分菌株在中性或偏碱性的条件下生长好，特别是Dry6、N4 和X5 菌株对碱性环境适应性强，在pH 值8.0 时生长最好；光照有利于菌株的生长；绝大部分菌株在3%NaCl浓度下生长良好，在5%～7% NaCl 浓度下除173 和86 菌株外，其它菌株都能生长，即对盐份的耐受性较好，且9 个菌株均为产碱菌；除173 菌株是兼性厌氧细菌外，其它都是好氧性细菌。

The cluster analysis showed that at level 0.73, 77 tested strains were clustered into 4 groups: A1: strains of Lactobacillus casei (35.06% of the strains), A2: strains of Lactobacillus fermentum (61.04% of the strains), A3: strains of Lactobacillus helveticus and A4: strains of Lactobacillus plantarum.

聚类分析表明，在0.73的水平上，77株乳杆菌共分为4大类群：干酪乳杆菌群A1、发酵乳杆菌群A2，瑞士乳杆菌群A3和植物乳杆菌群A4。

Results: Cultures for Mycobacterium were positive in a total of 2657 people, among them 1848 strains (69.55%) were Mycobacterium tuberculosis, and 809 strains (30.45%) were Nontuberculous Mycobacterium. Among the 1848 strains of Mycobacterium tuberculosis, 337 strains (18.24%) were drug resistant, and 75 strains (4.06%) of them were resistant to both Isoniazid and Rifampin which made them multi-drug resistant. Among the 548 strains of Nontuberculous Mycobacterium, 453 strains (82.66%) were drug resistant. There were a total of 261 rapidly growing mycobacteria, and 242 (92.72%) of them were drug resistant.

结果：分枝杆菌培养阳性共2657菌株/人，其中1848株(69.55%)为结核分枝杆菌，809株(30.45%)为非典型结核分枝杆菌感染。1848株结核分枝杆菌中，337株(18.24%)有抗药性问题，其中75株(4.06%)同时对Isoniazid和Rifampin有抗药性为多重性抗药菌。548株非典型结核分枝杆菌中453株(82.66%)有抗药性问题；快速生长菌群共261株，其中242株(92.72%)有抗药性问题。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

Results We isolated 2 strains of Bdellovibrio sp.(5#-12 and 5#-sh06) from sea mud of Shenzhen bay. Both strains grew between 20℃ and 35℃, with 25℃ and 30℃ as optimal temperature for 5#-12 and 5#-sh06, respectively. They grew between pH 6.1 and 8.6, and the opti℃mal pH for both was 7.2. Lysis experiments on 58 strains of pathogens were conducted and the results showed that 5#-12 and 5#-sh06 lysed 46 and 48 strains, corresponding to 79.3% and 82.8% of lysis abilities. Taken both two Bdellovibrio strains together, they lysed 96.6%(56 strains) of tested pathogens and 100% of tested vibrios (39 strains).

从深圳湾海泥中分离出2株蛭弧菌，分别命名为5#-12和5#-sh06，它们可在20℃~35℃范围内生长，最适温度分别是25℃和30℃；生长pH范围6.1~8.6，最适pH均为7.2；2株蛭弧菌可分别裂解46和48株试验菌，各占总试验菌株数（58）的79.3%和82.8%；联合2株蛭弧菌，可裂解56株试验菌，占总试验菌株数的96.6%；同时，它们一起能将所有试验弧菌裂解。

The four experimental virus strains and the three collate virus strains were divided into two groups, and belonged two evolutionary branches. Although ADV-DL1, DL2 and DL3 were all belonged to the Da Lian separated strains, they had the ulterior genetic relationship. The nuclear sequences of the obtained experimental ADV virus strains, ADV virus collate virus strains and typify virus strains of other four Parvovirus generic were cladogram analyzed with the Clustal W method of software DNA Star.

结果表明，ADV-Utah与四个实验毒株的序列同源性较高，同源率为92.9-93.4％，ADV-G、ADV-SL3与实验毒株的同源性较低；四个实验毒株和三个参考毒株被分别划归为两个组，分属于两个进化分支；实验毒株中ADV-DL1与DL2和DL3虽然同为大连分离株，却表现出较远的亲缘关系。

The result of the antibacterial and antifungal assay shows that thirty five strains of the endofungi (58.3% of total tested strains) have antibacterial activities, the active strains mostly belong to sterile groups (51.4% of active strains), Penicillium (8.6%), Aspergillus (8.6%), Fusarium(8.6%); forty nine strains (81.7% of total tested strains) have antifungal activites, most of them belong to sterile groups (44.9% of active strains), and Gloeosporium (17.1%).

结果 共有35株内生真菌具有抗细菌活性，占待侧菌株总数的58.3%，其中以不产孢类、青霉属、曲霉属、镰孢属为主，分别占活性菌株总数的 51.4%、8.6%、8.6%、8.6%；共有49株内生真菌具有抗真菌活性，占待侧菌株总数的81.7%，其中以不产孢类和盘长孢属为主，分别占活性菌株总数的44.9%、17.1%。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。