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To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.562 5-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。

In the Presence of His Father He lived on earth without strain from babyhood to His death on the cross.

神悅納了他整個生活的奉獻，在他的行為動作之中，沒有分別什么是他自己的，什么是天父的。

Ravel also sequenced the genome of a Bacillus subtilis strain that was found in one of the letters.

瑞福对于在其中一封信中发现的枯草芽孢杆菌也进行了测序。

Keep your lower back flat against the bench to prevent any strain.

保持您的腰部与凳子持平，以防止任何的压力。

Data on mutant strains includes detailed information on affected gene, mutant allele and genetic background (DNA origin, gene targeted, host and backcross strain background).

突变系的数据包括对影响的基因、突变等位基因和遗传背景(DNA起源，基因靶向，宿主和回交系背景)的详细的信息。

After repeat 10 generations backcross to get pure B6-strain background Gas7 knockout mice.

接著将Gas7基因剔除鼠和野生型的B6老鼠做交配，以期得到纯品系的Gas7基因剔除鼠。

More than 90% of the total incidence of typhoid and paratyphoid were typhoid previously, as the widely use of Vi bacterin, in the middle of 90s in 20th century, the popular strain changed, in some provinces the cases of typhoid decreased, but that of paratyphoid increased largely, with the outbreak trend from spot to part and from inshore provinces to backland provinces. In recent years, paratyphoid becomes the main infectious diease in some areas, and ofen develop to serious epidemic situation. So setting up a rapid and accurate detecting metod is necessary.

以前我国伤寒发病占伤寒副伤寒总发病的90%以上，后来由于伤寒Vi疫苗的广泛使用，20世纪90年代中期，流行菌株发生转变，我国部分省份伤寒病例逐渐下降，而由甲型副伤寒沙门氏菌引起的副伤寒病例却大幅上升，并呈现由散发到局部暴发，由沿海省份向内地省份推进的流行趋势，近几年来成为我国一些地区传染病突发事件的主要病种，常发展成重大疫情，急需建立快速、准确的诊断方法。

In a commercial nursery using the peat moss-plug system, these three strains also showed their ability to promote seedling growth following either seed bacterization or peat moss drenching, and their populations in peat moss maintained at high levels (except strain RS65 for 30 min seed soaking ) within 21 days after seeding. The above 14 strains that were capable of promoting tomato growth were tested for their ability to control bacterial wilt of tomato.

自台湾中部不同地区数种作物分离得到之396株根栖细菌，被覆於番茄种子后，利用套袋塑胶培养皿之系统及含泥炭土栽培介质之穴盘系统於生长箱筛选，共有 14株菌株在重复筛选过程中均能提高种子发芽率及促进根生长之效果，其中RS4、RS65及RS70三菌株表现最佳，可增加根长度、茎长度、植株鲜重及乾重(RS65菌株除外)。

The results indicate that the critical strain of bagasse Kraft black liquor can be up to 10%.

实验结果表明，蔗渣浆黑液多聚物的临界应变最高可达到10％。

It has about 300 to 400 baleen plates instead of teeth which it uses to strain food from the ocean water.

它拥有约300至400名baleen板，而非牙齿，它的用途应变食物从海洋的水。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新