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This strain was deposited in China General Microbiogical Culture Collection Center, named Bacillus subtilis NX-2 with the accession number of CGMCC No. 0833. Assimilability to carbon source by NX-2 was unique.

本论文主要工作如下:以L-谷氨酸分离培养基为特定的"筛子",通过大量的筛选和工作,获得了一株高产γ-PGA的菌株枯草芽孢杆菌N X-2。

During the previous analysis of asphalt pavement structure with cracks, the stress intensity factors were usually calculated approximatively using plane strain model for some limitation. However, the assumptive load of this model is not coincident to the practical load.

在以往分析含裂缝的沥青路面结构时,由于条件的限制,通常是假设沥青路面结构近似为一平面应变模型,但是这种模型所假设的荷载与实际作用的荷载有较大的区别。

So the results made it clear that this phytoplasma strain is one of the members of Aster yellows phytoplasma group (16SrI-B group).

基本确定了这两种植原体株系属于翠菊黄化组B亚组(16SrI-B,rp-B)。

Coli . The clones were verified by restriction enzyme digestion and PCR. Sequence and homology analyses showed that this phytoplasma strain shares high homology (99.0%) with the phytoplasma of Aster Yellows that belongs to 16SrI group.

通过酶切、PCR鉴定,对筛选得到的重组阳性克隆进行核酸序列测定及同源性比较分析,结果表明其与植原体16SrⅠ组中的西方翠菊黄化植原体同源率为99%。

But it is obviously under 97.0% with other groups. With further comparing the rps3 gene of ribosomal protein gene for the CWB-YN strain,the results show that these strains shared highest homology (98.1% and 100%)with Aster yellows and Periwinkle yellows.

进一步比较CWB-YN株系核糖体蛋白基因rps3基因编码的氨基酸序列同源性,结果与16S rI–B亚组的翠菊黄化病植原体(Aster yellows,AY)和长春花黄化病植原体(Periwinkle yellows,PY)的同源性最高,分别达98.1%和100%。

In the term of unloading and opposite side loading, let the stress-strain curve have the same form of skeleton curve, the curve uses the last unloading point as its starting point, and uses soil's ultimate stress level as its asymptote. Then the hysteretic curve is constructed.

在卸载和反向加载时,采用与骨架曲线相同的函数形式,并以前一次卸载开始点作为起点,以土体的极限应力水平作为渐近线,构造出土体动力本构模型的滞回曲线。

The first chapter gives the summary of basic theories of EOS for solids, including often-used thermodynamic theories and phenomenological expressions during the study progress of EOS along with the finite strain theory. Furthermore, from the view of solid-state theory the general expression of EOS of solids P =Pc+Pn is given, and the microcosmic mechanism of compression for solids, i.e., the interactive potential energy between atomics, is also analyzed, subsequently several different forms of cold energies and pressures are derived.

第一章,给出了物态方程的基本理论概要,总结了EOS研究中最常用的热力学理论和唯象关系式以及有限应变理论,并从固体理论角度给出了固体物态方程的一般形式P =Pc+Pn,分析了固体压缩的微观机理——原子间相互作用势,由此导出了几种不同形式的冷能和冷压。

Staphylococcus aureus Staphylococcus aureus with inhibition of 14 species (of the total strain of 87%).

对金黄色葡萄球菌金黄色葡萄球菌具有抑制作用的有14种(占总菌株的87%)。

The effect of uneven distribution of strain during compression on dynamic recrystallization and grain size of austenite was analyzed.

分析了热模拟变形过程中的非均匀应变对奥氏体动态再结晶及晶粒尺寸的影响。

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因,用PCR、测序鉴定缺失菌株,通过动物实验观察clpE基因缺失株毒力改变情况,同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力,最后用实时荧光定量PCR分析自溶素(major autolysin A,lytA)、表面黏附素A(pneumococcal surface adhesion A,psaA)、溶血素(pneumolysin,ply)、肺炎球菌表面蛋白A(pneumococcal surface protein A, pspA和神经氨酸酶(neuraminidase, nanA)的表达。

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The term at least trumps "intravaginal pouch," a phrase suggested in lieu of "female condom" by an FDA panel tasked in the early 1990s with reviewing an early prototype of the women's contraceptive.

不过,该术语至少着重体现了"阴道内的小口袋"这一概念,所以,FDA专家小组在20世纪90年代在检验前期一款女用避孕用具时就建议使用该词来代替"女用避孕套"。

She had had everything a girl could have: kindness, admiration, bonbons, bouquets, the sense of exclusion from none of the privileges of the world she lived in.

凡是一个女孩子可能有的一切,她统统都有:友谊,赞美,糖果,鲜花,她享有着她所生活的世界中的一切权利。

The yelling of each remark rolled over people's tongues like a snowball , getting bigger and clumsier as it w t and passing from one end of the boat to the other.

每句话全船传喊着,雪球似的在各人嘴边滚过,轮廓愈滚愈臃肿。