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The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

The objectives of this paper were to investigate the simulation and design of continuous multiple-station rolling forming process for a U-Section Steel Bar by CAE software-DEFORMTM Due to the limitation of elements number using a short strip 120mm went through all the stations to simulate the real continuous rolling forming on factory The roller gap was fixed as 0 30mm roll speed was 20 rad/sec and progressive angles were all equal for each rolling process which were the formed angle divided by by the number of station Three rolling processes were selected with 6 9 and 12 stations respectively During simulation processes two cases occurred Case A the strip was through a single station Case B the strip across two stations Because the strip laterals side was stretched and twisted during the process The model of case B closer to the practical process than case A and the calculated stress and strain on the tip of U section for case A were lower than these for case B by up to 15% From the result of case B for each process the stress for 12-station process was smallest 9-station process second and 6-station process next Because less-station can save larger cost and forming time but the bigger stress and strain for 6-station caused wrinkles on lateral sides not found on 9-station and 12-station Therefore 9-station process was a more suitable design than 12-station process smaller stretched and twisted and stress The progressive angles for 12-station was 7 5?

本文主要是以DEFORMTM有限元素软体模拟U型钢片连续道次成型与单一道次成型以确认单一道次模拟的是否可取代连续道次模拟之研究。因DEFORMTM 软体对於网格元素上的限制故使用较短素材120mm通过所有的成型站以模拟工厂实际连续成形方法。辊轮间隙固定为0 3mm；辊轮转速为20 rad/sec；弯曲角度的方法为将成型角度平均除以成型站数。有三种辊轮成型方式被选择分别为6站，9站，与12站。在模拟的过程中有两种现象会产生 Case A，素材通过单一成型站。Case B，素材会通过两个成型站。因为在模拟过程中素材的两端会有拉应力与扭力。Case B的模拟比Case A像实际的制程，并计算应力与应变在U型断面的Tip部中可发现Case A比Case B低约15%。从Case B的每站的结果可以得知，12站的成型应力最小，9站次之，6站最后。

Based on the axle load spectrum, temperature distribution and strain regression models, the pavement strain distribution in a year was calculated.

根据轴载谱、温度分布及应变回归模型，对不同路面结构全年的最大应变分布进行了分析。

A rigid-plastic FEM program for the analysis of working piece deformation in single pass hot bar rolling was developed. Combining with the analytical model, the strain and strain rate of hot rolled bearing steel GCr15 bar during roughing and intermediate rolling processes was calculated and compared with the real values.

应用刚塑性有限元法开发了轧制过程单道次变形分析的通用程序，与解析法结合对实际热连轧生产GCr15棒材的粗轧和中轧过程中的应变－应变速率进行了计算值与实际应变的对比。

HIV-1B 'strain is endemic in Asia's three major HIV-1 strain of the blood collection in China's central region HI

HIV-1B'毒株是亚洲流行的HIV-1三大主要毒株之一，在我国中部地区采供血HI

Measured the basic physical parameters of unsaturated soils in Three Gorges Reservoir area; 2 measured the initial sample matric suction using the unsaturated triaxial testing system, which can be used to evaluate the unsaturated soil strength and stress; 3 the drying and moisture absorption experiments were executed using the Tempe apparatus, and got the Soil-Water Characteristic Curve, permeability coefficients of every stress level were simulated according to the parameters from the SWCC; 4 the unsaturated soil strength tests were carried out using the GDS triaxial testing system, then got the Mohr circle and shear strength parameters; the results from different stress paths were compared; meanwhile, the axial strain-stress ratio-volume strain curve of different matric suction and different radial pressure was got, as well as their relationship; The research, which provided the necessary data and reference for Three Gorges Reservoir area geologic disaster, is the basic component of Three Gorges Reservoir geologic disaster prevention and research, and experimental results will be used directly in the project.

1量测了三峡库区非饱和土的基本物性参数； 2用非饱和土三轴试验仪量测了试样的初始基质吸力，为评估非饱和土的强度和应力水平提供参考； 3用Tempe仪和体积压力板仪进行了非饱和土的干燥和吸湿试验，得到了土-水特征曲线，并根据其参数拟合出各级吸力状态下对应的渗透系数； 4用GDS三轴仪进行了强度试验，得到了Mohr圆和抗剪强度参数；并比较分析了不同应力路径下试验结果；同时得到不同吸力和不同围压下轴向应变-应力比-体应变的关系曲线和其相互关系。该研究是三峡库区地质灾害防治研究工作的基础性工作，其结果将直接应用到工程实际中，为三峡库区地质灾害提供必要的数据和参考。

Additionally, with the 100 mm concrete layer, the effects of minimum thickness of the asphalt layer and a change in the temperature on the strain were not significant, while the increase in strain with the increased load was also relatively smaller.

此外， 与100毫米的影响混凝土层沥青面层厚度的最小变化和温度影响不显著，而应变的增大在紧张的负荷也相对增加小。

The rigorous solutions are derived for the consolidation problems of soils with drain wells through soft clay layer by use of the Merchant model under both free strain and equal strain conditions and in consideration of the effects of both well resistance and smear action.

本文基于Merchant模型分别推导了自由应变条件和等应变条件下打穿软粘土层的竖向排水井地基粘弹性固结问题解析解，同时考虑了井阻和涂抹作用的影响。

Analyzing the calculating process of belt conveyor with high angle theoretically, starting from its theoretic calculate the cross sectional area of materials and peripheral force, shaft power of drum, power of motor, using point by point method to get the resultant strain of every drum and torsion of driving drum, calculating the maximum strain and the maximum reverse stopping torque, to give the support on theory for using belt conveyor with super-long distance, high angle under the coal mine.

本文针对长运距、大倾角、高强度带式输送机设计过程中存在的问题，从四个方面对大倾角带式输送机进行分析研究：从理论上解析大倾角带式输送机的计算过程，从大倾角带式输送机的理论入手，详细计算物料的横截面面积、圆周力、滚筒轴功率和电机功率，利用逐点计算法计算各个滚筒的合张力以及传动滚筒的扭距，计算出胶带的最大张力和最大逆止力矩，给长运距、大倾角皮带机在煤矿井下的使用给予理论上的支持。

In this modified model, the effect of the stress concentration on the end stress in the fiber end region is considered, the average matrix strain is used to substitute for the so-called far field strain in the previous shear lag model. The change of the deformation states from elasticity to plasticity is considered and the interfacial shear stress and fiber axial stress are derived.

修正的模型合理考虑了纤维根区域应力集中对根应力的影响；用基体平均应变代替了经典剪切滞后模型中的远场应变；考虑了基体变形状态从弹性到塑性的变化，给出了塑性区上的表面剪切应力和纤维轴向应力的表达式。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。