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A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

The recombinant vaccine strain C501 had a series of biological characteristics silimar to the parent vaccine strain C500. It is likely that C501 could be adapted to develop multivalent recombinant Salmonella vaccine against both infections with S. enterica serovar Choleraesuis and toxigenic P. multocida.

本研究利用Asd+平衡致死系统的原理构建表达T+Pm保护性抗原重组猪霍乱沙门氏菌弱毒菌株C501，为进一步开发猪萎缩性鼻炎-副伤寒的双价基因工程疫苗奠定基础。

Different concentrations of rH-NS were added into the culture medium of the hns transposal mutant strain 1-21 and its parental strain 4074, and biofilms were quantified using the microtiter plate biofilm assay.

将不同浓度的rH-NS添加到hns突变株1-21及其亲本菌株4074的培养基中，用微孔板法测定生物被膜的形成。

Equiaxed and bigger grains are produced after C.P.Ti is deformed under the high temperature. Besides, many tiny subgrains were found in some of the metallurgical structure. The dislocation reaction caused by the deformation under the high strain rate generates ductile cellular metastructures, while the equiaxed cellular metastructures are generated under the low strain rate. The metallurgical structure of TC4 alloy including lath-shaped hexagonal martensite and trimetric martensite only exists in the higher temperature. The homogeneous, tiny and reticular grain shown after extreme high temperature deformation.

在高应变速率下变形引起的位错反应形成拉长的胞状亚结构，而低应变速率下变形则形成等轴的胞状亚结构；TC4合金在特高温变形条件下其金相组织呈网篮状，显微组织几乎全部为均匀细小的晶粒，同时出现了板条状六方马氏体；在温度尤其高的情况下还形成了斜方马氏体，变形引起的位错反应形成六方位错网络，在1200℃以下的变形易产生孪晶。

If the height of bump is higher, the radius of bump is smaller, and the thickness of wire , substrate and chip is thicker, the strain of package without underfill is smaller. If the height of bump is shorter, the radius of bump is bigger, the thickness of wire is thinner, and the thickness of substrate and chip is thicker, the strain of package with underfill is smaller.

在应变方面，较高的凸块高度，较小的凸块半径，较厚的线路、基板和晶片厚度将对未填胶构装体有较小的应变值；另外较矮的凸块高度，较大的凸块半径，较薄的线路，较厚的基板和晶片厚度将对填胶构装体有较小的应变值。

Biocontrol efficiency of Bacillus subtilis B6 strain and Trichoderma virid strain T23,in combination,was 82.22% in the greenhouse experiment,and 20.3% and 48.9% higher than treated groups single with B6 and T23 respectively.

在对甜瓜枯萎病温室防效试验的研究中发现，内生枯草芽孢杆菌B6菌株与绿色木霉T23菌株复合处理的相对防效达82.22%，比B6和T23的单独处理分别提高32.8%和146.7%。

With different culture background, formed two different ferment technology,course mixed ferment and pure-blood ferment, mixed ferment was provided with the quality of multiple strain accretion, enzyme system mutual complementarity, simplified equipment, suited to produce composition complexity and exigent flavour traditional zymolytic food of our country,pure——blood ferment had the quality of strain singleness,rare pollution, suited to produce composition singleness,exigent puritg alcohol and monosodium glutamate.

中西方文化底蕴不同，形成混含发酵与纯种发酵两种不同发酵工艺路线的历程。混合发酵具有多菌共生，酶系互补，省工节能，简化工艺设备，克服中间生成物浓度过大的特点。宜于生产成分复杂，风味要求高的我国传统发酵食品，纯种发酵基质，菌株单一，污染少，宜于生产成分单一，纯度要求高的酒精，味精等产品。

Based on the unloading stress-strain relationship obtained from the unloading stress path experiment reflecting the stress-strain characters of soil in the active area behind the wall, the relationship between the friction angle and wall movement is established under nonlimit state.

根据反映墙后主动区土体应力-应变性状的卸荷应力路径试验确定的应力-应变关系，建立非极限状态摩擦角与墙体位移的关系。

The strain was obtaind by screening and acclimating. The characteristic of the oxygen-resistant suitability for commercial production of the strain is better than the original for one.

通过筛选和驯化所得到的耐氧型菌株与出发菌株相比，耐氧特性明显提高，能更好适合生产的需要。

In this article the bulldozing shovel-soil contact system was studied by two-dimensional finite element method. The distributions of the stress and strain in soil were analyzed separately under plane stress situation as well as plane strain situation.

本文运用二维有限元方法对推土铲－土壤接触系统进行了研究，分别分析了平面应变问题和平面应力问题中土壤所产生的应力及应变分布状况；对比了它们在两类平面问题中的差异。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。