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Two strains of bacteria, named as TJ-1 and TJ-2, were isolated from enrichment with crude oil as a sole carbon source. Strain TJL-1 and Strain TJL-2 were isolated with hexadecane as a sole carbon source. The 16SrDNA identified results showed that the four strains of TJ-1, TJ-2, TJL-1 and TJL-2 were Bacillus pumilu , Acinetobacter venetianus , Oceanobacillus Picturae , Bacillus pumilus , respectively.

结果表明，利用2216E平板从以原油和正十六烷为碳源的富集液中各得到两株菌，分别为TJ-1、TJ-2和TJL-1和TJL-2，分子生物学方法鉴定结果表明，这4株菌分别为短小芽孢杆菌、威尼斯不动杆菌、 Oceanobacillus Picturae 和短小芽孢杆菌。

A diverse range of effects:on protein solubility and secretion was observed,and these effects were highly dependent on the particular chaperone.In the host strain over-expressing chaperone SecB harboroing pSecB,the amount of total periplasmic proteins was increased by about 71％and the activities of alkaline phosphatseand GL-7-ACA acylase(GL7A)were increased by about 54％and l.5-fold respectively.In the host strain over-expressing chaperone GroEL bearing pGroEL,the amount of total periplasmic proteins was increased by 52％,and the activity of penicillin G acylasewas in creased by about 76％;about 90％of Hexameric Calcitonin(Cal6)inclusion body and l5％of MS2interleukin3(MS2-HIL3)inclusion body became soluble respectively.All the data were compared with the parental strains un-bearing plasmdis pSecB or pGroEL.

在过量表达SecB的宿主菌中，周质空间分泌蛋白总量较对照组提高了约71％，GL-7-ACA酰化酶在周质空间酶的活力较对照组提高了约1.5倍，碱性磷酸酯酶在周质空间酶的活力较对照组提高了约54％；在过量表达GroEL的宿主菌中，周质分泌蛋白总量较对照组提高了约52％，青霉素G酰化在周质空间酶的活力较对照组提高了约76％，鲑鱼降钙素六聚体的可落性组分的比例由原来的45％增加到约90％，而MS2-人白介素-3融合蛋白的包涵体有约15％转变为可溶性组份。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

Hydric and hydration gradients are measured by using the ''slice test'' and strain gradients are measured at various positions in the material layer using embedded strain gauges.

氢气和水化梯度是衡量使用''''切片检验和应变梯度测量在不同的岗位材料层使用嵌入式应变计。

The protoplast fusion between hyperparasitic Streptomyces strain F46 and Actinomyces strain SC11 was studied in order to improve the effect of wild actinomyces strains to control plant diseases, solve the problems in application of the biocontrol strains, explore the method of protoplast fusion and get the recombinants with some excellent characteristics of both parents.

为了提高野生生防放线菌株的防病效果，克服以往生防菌应用方面存在的诸多弊端，探索简单易行的人工改良生防放线菌的途径，我们选用重寄生链霉菌F46和放线菌SC11作为亲本，进行了双亲原生质体融合研究，以期得到整合双亲多种优良性状的工程菌株。

The rearranged idiotypic CDR3 fragments coding TCR Vβ8 of the Jurkat cell strain and TCR Vβ2 of the Molt4 cell strain were amplified using RT-PCR, and then were cloned into pIRES vector respectively.

用RT-PCR方法扩增特异性重排的Jurkat细胞TCR Vβ8 CDR3序列和Molt4细胞TCR Vβ2 CDR3序列，分别克隆到真核表达载体pIRES中。

The recombinant plasmids, pIRES-Jurkat Vβ8 and pIRES-Molt4 Vβ2①/②, containing idiotypic TCR Vβ8 frgment of Jurkat cell strain or idiotypic TCR Vβ2 frgments of the Molt4 cell strain were developed.

构建了Jurkat细胞独特型TCR Vβ8 DNA重组质粒pIRES-Jurkat Vβ8和Molt4细胞独特型TCR Vβ2 DNA重组质粒pIRES-MOLT4 Vβ2①/②。

MAIN OUTCOME MEASURES: Strain at L5-S1, intervertebral disc and iliolumbar ligament was determined using strain gauge.

主要观察指标：L5峡部贴应变片，用应变仪测量L5~S1峡部、椎间盘和髂腰韧带应变。

Incurrent research, aiming at the rupture problem engendered in the shooting process of acertain 5.56mm automatic rifle, the strain and stress distributions on the cartridge caseunder the action of axial pull have been analyzed. Based on the theories of elastic-plasticmechanics and contact mechanics, a mathematic and physical model of the cartridge-caseand chamber system has been set up; and based on the platform of the general softwarepackage APDL of ANSYS for finite element analysis, a parameterized model of thecartridge-case and chamber system has also been established. The method for nonlinearFEA has been adopted to analyze the stress distribution on the entire cartridge-case andchamber system, and the method of contact element has been employed to investigate thecontact stress between the cartridge case and chamber, and then the influences of frictioncoefficient and locking space on the static and dynamic stress and strain response of thecartridge case and chamber to the action of power gas have also been discussed. On thebasis of a lot of numerical tests, a kind of element Plane13, which is concentrated incoupled computation of heat and construction, has been used finally to simulate thetransient response of the cartridge-case and chamber system to the power gas. Throughloading the temporary thermal characteristics of explosive gas in the procedure of emulate, the corresponding transient thermal stress distributed on the entire system under thecombined load of transient thermal stress and transient pressure has been obtained. Owingto these comprehensive considerations, the numerical results obtained in this paperrepresent the actual situations comparatively exactly.

本文针对某5.56mm自动步枪在工作中出现断壳的问题，分析了弹壳在轴向拉力作用下的应力应变；基于弹塑性力学及接触理论，建立了弹壳弹膛系统的数学、物理学模型，在通用有限元ANSYS软件的参数化建模工具APDL平台上，建立了弹壳弹膛系统的参数化模型；采用非线性有限元法计算了弹壳弹膛系统的整体应力，引入接触单元法分析弹壳和弹膛的接触压力，探讨了在不同的摩擦系数和不同的闭锁间隙对弹壳弹膛静、动态响应的影响；本文进行了大量的尝试，最后确定采用热、结构耦合单元Plane13，在计算中加载了火药气体的温度历程，成功的获得了弹壳弹膛系统在瞬态的热载荷和瞬态的压力载荷同时作用下弹壳弹膛系统的热应力数值解，考虑因素全面，比较真实的反映了弹壳弹膛系统在火药气体压力和热载荷下作用的情况。

In this paper, the baroclinic pole in baroclinic theory is supposed to be membrane-element subjected to shearing stress and normal stress in plain section. With the help of truss model, it meets the equation of two-dimension stress, Moire's strain corresponding equation and double-axis intenerate strain-stress relationship of concrete. It describes the load-behavior and working property of reinforced concrete combined torsion members in the whole load-deformation process.

本文将斜压场理论中的斜压杆表达成在平面内承受剪应力和正应力的钢筋混凝土薄膜元，借助桁架模型，满足二维应力平衡条件、莫尔应变协调条件和混凝土的双轴软化本构关系，揭示钢筋混凝土复合受力构件在受力-变形全过程中受力行为和工作性能，为研究复合受扭构件的变形行为打下了坚实的基础。

Consumer preference ; fuzzy partial ordering relation ; condorect function

消费者偏好；模糊偏序关系； condorect函数

And she doesn't have a cat, she has a Mexican hairless.

而且她不养猫，她养了一只墨西哥无毛犬。