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The results show that this strain was a typical acidophile with optimal growth pH being 2.0 for hypha and pH 3.0-4.0 for the spore germination. Peptone was the most suitable nitrogen source which helped produce xylanase induced by xylan during stationary phase. The resultant xylanase reacted best at 50℃-55℃ and pH 3.5, and had activities 70% after 60 min maintenance under optimum reaction conditions.

结果表明：实验菌株嗜酸，菌丝生长最适pH为2.0，孢子萌发生长适宜pH为3.0-4.0；木聚糖诱导菌体在生长稳定期大量产生木聚糖酶，蛋白胨是菌体产酶的适宜氮源；菌株所产木聚糖酶属于酸性木聚糖酶，反应最适pH3.5、最适温度50℃-55℃，在最适反应条件下保温60min，残余酶活仍接近70%，适用于较强酸性的高温加工环境。

Aim For the purpose of making up the weakness in actinomycete strain which can produce alkaline cellulose at present.

目的为了弥补目前学术界在产碱性纤维素酶放线菌研究方面的薄弱环节。

A thermophilic actinomycete strain AO-Ⅲ was isolated from the composting process of pulp and paper mill sludge at the thermophilic stage.

高温放线菌是堆肥过程中微生态环境的重要组成成分，在好氧堆肥的升温期对有机物的分解发挥重要的作用。

Liverpool today confirmed Steven Gerrard faces at least another week on the sidelines as he continues to recover from a strain in his adductor muscle.

利物浦今天证实史蒂芬杰拉德面临至少一个星期的间隙，他继续等待恢复。

Methods Live at-tenuated rubella vaccine virus strain RA27 /3 was inoculated to the monolayer of human diploid MRC-5 cells at an appropriate MOI and incubated in serum-and protein-free CDM culture medium.

方法将风疹减毒活疫苗毒种RA27/3株按适当比例接种单层人二倍体细胞MRC-5株，使用无血清、无蛋白的CDM培养基培养病毒，收获病毒液，加入聚乙烯吡咯烷酮作为病毒稳定剂，制备疫苗原液，加入冻干保护剂，制备5批冻干疫苗，进行各项检定。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

When the elastic modulus is high, stress and strain are mainly concentrated at edge of the overlap region, while those in weld spot zone are relatively even; when the elastic modulus is low, stresses in the adhesive layer are small while stresses are concentrated to a certain extent at edge of the weld spot.

分析发现，胶粘剂弹性模量对接头中应力应变分布的趋势有明显影响，胶粘剂弹性模量高时，搭接区边缘附近区域应力应变高，焊点区应力应变值相对较小；弹性模量低时，焊点边缘处存在一定的应力集中，胶层中应力较小。

NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days;(2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days;(3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

NJWGY3665菌种接到葡萄糖营养培养基中，进行斜面培养，培养温度28-37℃，培养时间2-6天；(2)种子培养：用无菌水将斜面上培养的孢子制成单孢子悬液，并接种到种子培养基中培养，温度为28～32℃，转速为200rpm，培养2-6天；(3)发酵培养：将种子液接种到发酵培养基中培养，温度控制在28-37℃，pH控制在6.0-9.0，转速180-220rpm，发酵5-9天，得到链霉菌，采用离心分离的方法将菌丝体用甲醇或乙醇进行提取分离，再采用树脂方法进行精制，获得多肽类抗生素安来霉素。

The cost of each SmartTAG with Touch 'n Go card is assumed to be at RM75 and the deposit for the tag is RM20. The initial capital cost for providing SmartTAG would be high but if the distribution is done in stages, this would alleviate the strain on the company.The implementation of all lanes using SmartTAG would also reduce the company's staff cost and overhead (air conditioning at workplace, cashier machine) for ticket collection and receipt of money.

我们预计每个附有一触即通卡的精明通行器的成本是75令吉，而抵押金是20令吉，提供精明通行器的初始成本可能较高，但若分阶段进行反将减轻公司的压力与此同时，此措施也能减少公司的人资成本及开支如工作场所冷氣设备、收銀机等，然后把相关的工作人员转移其他部门，例如在休息站设立加额柜台，或处理行政工作。

The results show that the flow stress of both materials decreases with increase of temperature, and strain hardening rates at a temperature below 585 K are greater than that at the temperature above 585 K.

测试结果表明，不论是上引法连铸多晶铜还是连铸单晶铜，流动应力随温度的升高而下降，在温度低于585 K时，材料的应变硬化率明显大于在温度高于585 K时的应变硬化率。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。