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Selecting the optimum carbon sources, nitrogen sources and inorganic salt to get the optimum liquid medium recipe by the orthogonal test in three factors and four levers; Isolated toad gastrocnemius stimulated by pulse electricity was regarded as fatigue model and Clitocybe maxima mycelia was compared with Ringer solution to see what influence they had on muscle contract ability.

采用深层培养方法，筛选出较优的碳源、氮源、无机盐，设计三因素四水平的正交试验，筛选大杯伞的最适培养基；采用脉冲式电流直接刺激蟾蜍离体腓肠肌作为疲劳模型，观察大杯伞菌丝体和任氏液对蟾蜍腓肠肌收缩能力的影响。

Methods: The cells were treated with the different concentrations of Cobaltous chloride. The lactic acid dehydrogenase from the supernatant of the cells was detected by a biochemical analysis. The cells were co-transfected with a pGL2-eNOS-p vector while stimulated with Cobaltous chloride at the different concentrations and disposing time, and the transcription activity of human eNOS promoter was determined through using a double luciferase reporter gene system.

用含不同浓度氯化钴的培养基培养细胞，检测细胞培养上清中的乳酸脱氢酶含量；将已构建的pGL2-eNOS-p质粒转染HUVEC-12细胞，利用双荧光素酶报告基因技术检测在不同浓度氯化钴和不同作用时间下的eNOS启动子转录活性。

Further, with a semi-quantitative RT-PCR technique, the temporal expression of UuMAPKKK-like in U. unicinctus coelomic fluid cells was measured after stimulated by sulfide. The mRNA transcript of UuMAPKKK-like was low in the control and short time stressed (2 h, 6 h) groups, up-regulated gradually after 12h stimulation, and then reached its maximum level at 48h.

进一步采用RT-PCR技术对其在硫化物刺激前后的表达进行了检测，结果显示该基因在对照和应激后2h、6h的个体中表达较弱；刺激12h后表达量增高，并随应激时间增加，呈明显上调趋势。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

In order to investigate the effect of tumor necrosis factor α on the activation of astrocyte, the oligodeoxynucleotide was used to inhibit the production of TNF α from astrocytes stimulated by Coriaria Lactone.

为进一步探讨 TNF-α在星形胶质细胞激活中的作用，用 TNF-α反义寡核苷酸抑制由马桑内酯(coriaria lac-tone，CL )诱导的星形胶质细胞 TNF-α的产生，观察 NFκBp6 5核转位和 IκBα降解的变化。

Callus derived from petiole explants of Panax japomcus var. major produced three cyanidin glycosides as major anthocyanin pigments in darkness. Anthocyanin synthesis was remarkably stimulated by 2,4-dichlorophenoloxyacetic acid in combination with kinetin in MS medium. The pigment content of the callus reached up to 34. 14 mg/g fw.

从珠子参(panax japonicus var.major)叶柄诱导的愈伤组织在暗培养条件下主要形成3种花色甙色素，且均以矢车菊素为甙元。2，4-二氯苯氧乙酸和激动素的组合能明显地促进色素的合成，色素含量可达34.14mg/g fw。

Callus deried from petiole explants of Panax japomcus ar. major produced three cyanidin glycosides as major anthocyanin pigments in darkness. Anthocyanin synthesis was remarkably stimulated by 2,4-dichlorophenoloxyacetic acid in combination with kinetin in MS medium. The pigment content of the callus reached up to 34. 14 mg/g fw.

从珠子参(panax japonicus ar.major)叶柄诱导的愈伤组织在暗培养条件下主要形成3种花色甙色素，且均以矢车菊素为甙元。2，4-二氯苯氧乙酸和激动素的组合能明显地促进色素的合成，色素含量可达34.14mg/g fw。

Methods The Cynomorium polysaccharide and Cynomorium water extract were extracted and prepared chewable tablets. Wistar rats were randomly divided into normal control, aging model, VitE, Cynomorium polysaccharide chewable tablets and Cynomorium water extract chewable tablets groups. Dgalactose was injected to establish the aging model. After drug intervention, the proliferation activity of rat spleen lymphocytes stimulated by ConA was detected by MTT assay and the spleen index was measured. The pathological morphological changes of spleen were observed. The activity of superoxide dismutase, the content of malondialdehyde and nitric oxide in serum of rats were detected.

提取并制备锁阳多糖、水提取物咀嚼片；Wistar大鼠随机分为空白对照组、衰老模型组、维生素E对照组、锁阳多糖组、水提物组，以D半乳糖建立衰老大鼠模型，药物干预后，MTT法检测各组大鼠脾淋巴细胞对ConA刺激的增殖能力，检测各组大鼠脾脏指数，观察脾组织的病理形态学变化；并检测各组大鼠血清中超氧化物歧化酶活性、丙二醛及一氧化氮的含量。

Total cytoplastic mRNA of PBMC stimulated by anti-CD3 mAb at continuous time points was isolated and reversed to cDNA.

在共培养的12h，IL-2mRNA的含量显著上升并达到峰值14000copies／ml，随着培养时间的延长快速下降。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。