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Ten samples of slope-wash and colluvial deposits from a trench wall at Xiyangfang,Yanqing County,located in the northern margin fault zone of the Yanqing Basin,about 90 km northwest Beijing,have been dated by both green light stimulated emission and infrared stimulated emission.

对北京延庆西羊房古地震探槽西壁采集的 10个崩积物和坡面沉积物样品进行了细颗粒(4～ 11μm)多测片的红外释光和绿光释光测年。

Macrophages stimulated by ConA showed large size, fingerlike process and rich mitochondria. Macrophages stimulated with ConA showed a high ability of phagocytosis.

ConA刺激后，可增强Mφ产生NO、TNF-α、IL-1，这些效应分子在体内可能作用于其它细胞，调节机体的免疫活化状态。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型，通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用；采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用；利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

Cytokine-producing CD4＋ T cells were quantified by 3-color flow cytometry after bring stimulated with phorbol myristate acetate and ionomyccin. After being stimulated with PHA, the levels of IFN-γ and IL-4 in culture supernatants were measured by ELISA.

同时分离外周血单核细胞，经植物血凝素刺激培养后，酶联免疫吸附方法检测培养上清液中IFN-γ和IL-4水平。

Cytokine-producing CD4＋ T cells were quantified by 3-color flow cytometry after bring stimulated with phorbol myristate acetate and ionomyccin. After being stimulated with PHA, the levels of IFN-γ and IL-4 in culture supernatants were measured by ELISA.

同时分离外周血单核细胞，经植物血凝素刺激培养后，应用酶联免疫吸附方法检测培养上清液中IFN-γ和IL-4水平。

The purpose of this study is to evaluate the clinical effect of "stimulated" osteoperiosteal pedicle grafts and root demineralization with citric acid in reconstructing periodontium lost by gingival recession and to observe the histological response of periosteum to controlled surgical stimulation and the healing after experimental stimulated osteoperiosteal pedicle grafts and root demineralization in animal, especially regeneration of alveolar bone.

为了评价牙周刺激带骨瓣移植结合根面枸橡酸处理，在整复由于牙龈退缩所丧失牙周组织方面的临床效果：观察成年动物的骨膜对有控制的外科穿刺性损伤及动物实验性刺激带骨瓣结合酸处理术后的组织学反应，特别注意牙槽骨的再生情况。

Orexin A(100 and 1000 nM) stimulated the release of GnRH from hypothalamic explants harvested from prepuberal swine.However,only 1000 nM orexin B could stimulated the release of GnRH from the hypothalamic explants harvested from prepuberal swine.

五、Orexin对性成熟青年母猪下丘脑组织-垂体-卵巢轴作用的体外研究为探讨orexin在生殖调节中直接作用的靶位点，我们在体外培养了猪下丘脑脑片、垂体和卵巢细胞，研究了orexin A和orexin B在体外对下丘脑、腺垂体和卵巢GnRH、LH和E2释放的影响。

Methods M. penetrans was cultured and its LAMPs were extracted. Mouse macrophages were stimulated with M. penetrans LAMPs to analyze the production of nitric oxide. Cell prolification was detected in M. penetrans LAMPs-stimulated mouse macrophages by MTT assay.

用SP-4培养基培养穿透支原体并提取其脂质相关膜蛋白，用提取的脂质相关膜蛋白刺激小鼠巨噬细胞，以ELISA法检测NO的产生，并用MTT法检测其对小鼠巨噬细胞增殖的影响。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

After administration of L-N〓-nitro-Arginine, a specific antagonist of NOS, it was found that the basal and stimulated NO release of blood vessels (aorta, internal carotid, renal and mensenteric artery) was decreased further. The increase of coronary blood flow stimulated by Ach was also reduced further, while the platelet aggregation degree and erythrocyte aggregation index increased significantly. Pathological examination indicated that aortic endothelium was destroyed, the blood vessels in cerebral, cardiac and renal tissues were hypertrophied further and some were almost occluded. The above fin dings resulted in SHR feeded with L-NNA having high incidence of stroke rate and high degree of renal cirrhosis when compared to control SHR. The infarct and hemorrhagic focuci in cerebral tissues, infarct focuci in cardiac tissues, atrophy and hyalinedegeneration of renal glomeruli and degeneration even necrosis of renal tubuli in renal tissues were al so found. The increase ratio of brain and heart to body weight and decrease ratio of kidney to body wei ght further demonstrated that the cerebral, cardiac and renal injuries were aggravated.

在上述结果的基础上进一步给予SHR NOS特异性拮抗剂左旋亚硝基精氨酸后，血管(胸主、颈内、肾及肠系膜动脉)NO基础释放及激动剂刺激释放进一步减少，Ach刺激冠脉流量的增加和胸主动脉内膜NOS活性也进一步减少，血小板聚集程度及红细胞聚集指数显著上升，病理切片显示胸主动脉内皮细胞破坏明显，脑心肾组织内的血管进一步增厚甚至几乎阻塞，导致饲L-NNA的SHR缺血性及出血性脑卒中发生率显著高于对照SHR，脑体重比显著升高；心肌内出现明显的缺血梗塞疤痕灶，心体重比显著升高；肾小球明显萎缩、玻璃样变性，肾小管也变性甚至坏死，使肾硬化程度显著增加呈颗粒性固缩肾，肾体重比也显著减少。

Metrorrhagia is also gynaecological problems of common diseases.

崩漏是妇科常见病也是疑难症。

WordFTP gives you the convenience and timesaving you need.

wordftp让您的方便和省时您所需要的。