查询词典 sticking

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

These feed steam through the straight passages in the piston to the two working volumes 3 and 2 at appropriate times, and force is exerted on the spring-loaded shutter sticking out of the cylinder.

这些饲料的蒸汽通过直通道，在活塞向两个工作卷3和2个在适当的时候，和武力施加于弹簧加载快门伸出的圆柱。

Arrive from mouth of school front door between 48 education building on the road surface that is less than 1 kilometer sticking ten marked red arrowhead, writing above " E road is connected " cup the model of written characters of contest of application of innovation of business affairs of network of undergraduate of the 2nd whole nation; In education building, everywhere is seen or be business suit change is walked on, or it is to wear those who unite advertisement unlined upper garment " stranger ", ground of their in threes and fours gets together in the corridor in classroom out and stair well, discussing livelily.

从学校正门口到48号教学楼之间不到1公里的路面上贴着十几个醒目的红色箭头，上面写着&e路通&杯第二届全国大学生网络商务创新应用大赛的字样；教学楼里，随处可见或是西装革履，或是身着统一广告衫的&陌生人&，他们三五成群地聚在教室外头的走廊和楼梯间里，热闹地讨论着。

Under the new circumstances, sticking to our school motto as well as constructing 'freedom, independent, and self-creative' academic atmosphere is more able to embody our fundamental task on basic education and our society's essential requirements to the quality of our talented students.

在新的环境下，坚持我们校训的同时，营造&自由、自主、自我创新&的学术氛围更能体现现阶段基础教育的根本任务，和当今社会对建设人才素质的本质要求。

Under the new circumstances, sticking to our school motto as well as constructing 'freedom, independent, and self-creative' academic atmosphere is more able to embody our fundamental task on basic education and our society's essential requirements to the quality of our talented students.Hereby express my blessing and acknowledgement, and I wish my Alma Mater to get better and better and make remarkable achievements forever.

在新的环境下，坚持我们校训的同时，营造&自由、自主、自我创新&的学术氛围更能体现现阶段基础教育的根本任务，和当今社会对建设人才素质的本质要求。

Sticking to the new school policy, a new organ which devotes in sustainable development – School of Urban Planning and Design - emerged with the basis on the two previous majors, regional planning and landscape design in Environment and CityCollege.

根据这一新的办学方针，一个致力于中国城乡可持续发展研究的新机构——城市规划与设计学院——便在原环境与城市学院的城市与区域规划和景观设计两个专业基础上应运而生。

To solve the problem of pump sand sticking in Wennan Oilfield, a high-efficiency anti-sand and anti-gas string is developed.

为解决文南油田高气液比井出砂易造成卡泵的问题，研制了高效防气防砂一体化管柱。

This paper mainly discusses the difficult points of the sticking technology of high temperature resistance fluoro-aether rubber.

阐述了耐高温氟醚橡胶粘接的难点，分析了传统工艺的缺点。

What's that tall building sticking high into the sky?

那个高耸入云的大楼是什么？

Basing on good credit standing, high quality, sticking inspection and better service, our company has standing a good image in society.

凭借着这些年来的良好信誉、高质量、严要求及周到的服务让我们在手袋加工和丝印这块领土上树立了良好的形象。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。