查询词典 sterilely

Adding Ficoll to centrifugalize, BMSCs were isolated, cultured and proliferated. The intestinal segment about 15 mm×5 mm was obtained sterilely from fetal duodenal papilla to colon, then muscular tunic and adventitia were peeled.

收集四五个月流产胎儿标本6例的四肢骨髓，Ficoll离心，分离、培养、扩增获取骨髓间充质干细胞。

METHODS: Full-thickness skin was sterilely obtained from rabbit ears. 2 cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

无菌条件下切取兔耳全层皮肤，将2 cm×2 cm皮片置于0.25%胰酶-EDTA消化液中去掉表皮，再置入曲拉通X-100溶液使真皮细胞完全脱净，磷酸盐缓冲液反复清洗，即为脱细胞真皮基质，4 ℃保存备用。

METHODS: Neonatal mouse brain tissues were sterilely obtained to prepare monoplast suspension.

无菌条件下取新生小鼠脑组织，制备单细胞悬液。

METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏，以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液，分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心，吸取界层细胞，添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

METHODS: Rat cerebral cortex was obtained sterilely, made into monoplast suspension by the mechanical separation, and then incubated in the serum-free MEM/F12 medium, supplemented with B27 neurotrophic additive for 1 week. Subsequently, cells were passaged.

无菌条件下取胎鼠大脑皮质，剪碎后机械分离法制作单细胞悬液，加入含B27神经营养添加剂的无血清MEM/F12培养基进行培养，1周后传代。

METHODS: Olfactory bulb was sterilely collected from rats, digested, and centrifuged. After removal of the supernatant, the olfactory bulb was resuspended with DMEM/F-12 medium containing 0.2 volume fraction of fetal bovine serum. The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence + serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁+无血清条件培养液纯化的实验。

ML of bone marrow was sterilely aspirated from the posterosuperior iliac spine of 36 healthy mongrel dogs. BMSCs were isolated and purified by Percoll density gradient centrifugation and cultured in vitro by adherence method.

选择健康杂种犬36只，无菌抽取犬骨髓液10 mL，经Percoll液密度梯度离心后贴壁法体外培养骨髓间充质干细胞，传2代后行5-氮胞苷诱导，5-溴脱氧尿苷标记备用。

All patients received a massive bone allograft that had been sterilely procured without secondary irradiation.

所有的病人都接受了经消毒而未进行二次辐射的大块同种异体骨。

METHODS: Bone marrow was sterilely isolated. BMSCs were isolated and purified by the density gradient centrifugation.

无菌条件下抽取猪骨髓液，密度梯度离心法分离纯化骨髓间充质干细胞。

All patients receied a massie bone allograft that had been sterilely procured without secondary irradiation.

所有的病人都接受了经消毒而未进行二次辐射的大块同种异体骨。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。