查询词典 sterile

The recombinant plasmid pBI121-VP6 was transformed into Agrobacterium tumefaciens strainLBA4404 by freeze-thawing method and the introduced into the sterile tube culture shoot of tobacco NC89 and baoding alfalfa by leaf disc infection method.

以冻融法将pBI121-VP6重组质粒导入根癌衣杆菌LBA4404，用叶盘法转化于NC89烟草和保定紫花苜蓿的无菌试管苗。

PHap1 was first transformed into Agrobacterium tumefaciens strain LBA4404 by electroporation first and then introduced into the sterile tube culture seedlings of different susceptible potato eultivars'Mira','Zhong-5-1'and'Jinguan', and tobacco cultivar NC89, respectively, by the leaf disc infection method.

以电激法将pHap1导入根癌农杆菌LBA4404，用叶盘法分别转化马铃薯青枯病感病品种米拉、中-5-1、金冠以及烟草感病品种NC89的无菌试管苗。

The Plant tissue raise is the plant sterile culture technology, has the totipotency theory according to the vegetable cell, the use plant body exsomatize organ (for example root, stem, leaf, stem point, flower, fruit and so on), the organization (for example cambium, epidermis, cerebral cortex, marrow department cell, endosperm and so on) or the cell (for example big spore, small spore, somatic cell and so on) as well as the protoplast, in aseptic and suitable artificial culture medium and illumination, temperature and so on under artificial condition, can induce injuries the organization, not to decide the bud, the adventitious root, finally forms the complete adult plant the discipline.

植物组织培养即植物无菌培养技术，是根据植物细胞具有全能性的理论，利用植物体离体的器官(如根、茎、叶、茎尖、花、果实等)、组织（如形成层、表皮、皮层、髓部细胞、胚乳等）或细胞（如大孢子、小孢子、体细胞等）以及原生质体，在无菌和适宜的人工培养基及光照、温度等人工条件下，能诱导出愈伤组织、不定芽、不定根，最后形成完整的植株的学科。

NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days;(2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days;(3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

NJWGY3665菌种接到葡萄糖营养培养基中，进行斜面培养，培养温度28-37℃，培养时间2-6天；(2)种子培养：用无菌水将斜面上培养的孢子制成单孢子悬液，并接种到种子培养基中培养，温度为28～32℃，转速为200rpm，培养2-6天；(3)发酵培养：将种子液接种到发酵培养基中培养，温度控制在28-37℃，pH控制在6.0-9.0，转速180-220rpm，发酵5-9天，得到链霉菌，采用离心分离的方法将菌丝体用甲醇或乙醇进行提取分离，再采用树脂方法进行精制，获得多肽类抗生素安来霉素。

It's a sterile Disney-fied walled garden surrounded by sharp-toothed lawyers.

他是一个像迪士尼乐园一样的世界—先消毒杀菌然后与世隔绝，最后再派一群尖牙厉嘴的律师当保镳。

DJ Products saw the need for stainless steel versions of its popular CartCaddy Dolly Pusher to assist workers in these and other industries that demand sterile environments.

DJ产品看到了其广受欢迎的CartCaddy多莉推不锈钢版本需要协助这些和其他行业的工人无菌环境的需求。

DJ Products saw the need for stainless steel versions of its popular CartCaddy Dolly Pusher to assist workers in these and other industries that demand sterile environments.

DJ产品认为有必要对不锈钢版本，其流行的CartCaddy多莉推，以协助这些工人和其他行业的无菌环境的需求。

Collected some suitable maize CMS-S type 〓 germplasm and selected avector expressing the 〓 gene 〓 was transferred to 〓 and 〓 by backcrossing,fertility Checking and self-pollinating. The genotype of sterile line 〓 was 〓〓. The genotype of restorer line 〓 was 〓. A pair of maize CMS-S〓 near isgenic lines 〓 and 〓 was established firstly in China.

征集了合适的玉米CMS-S型〓种质资源，选择了有效表达〓的载体材料，采用回交转育、育性鉴定和自交纯化，将作者培育的〓优良自交系转育成基因型为〓的不育系〓和基因型为〓的恢复系〓，在国内首次创建了玉米CMS-S的〓近等基因系〓和

A sterile liquid containing proteins extracted from cultures of tubercle bacilli and used ''.

结核菌'。''''。'素'。''从结核培养菌中提取含有蛋白质的无菌液体，用于肺结核测试

The gloved hand should not come in contact with the skin before the incision is made. For the extremities, a section of sterile stockinette is drawn proximally over the operative field. Then the stockinette is grasped proximally and distally and cut with scissors to uncover the area of the proposed incision. Its cut edges are pulled apart, and the area is covered by a transparent adhesive-coated material.

带手套的手在切皮之前不许与皮肤接触，肢体手术可应用弹力织物向近端套于手术区表面，然后抓住弹力织物的近端及远端，于木野处用剪刀剪开，将剪开的边缘拉开，在暴露的术野的皮肤上贴无菌的皮肤贴膜。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。