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Sensitivity comparison between the novel one-step RT-PCR and DAS-ELISA for PVX revealed that the former method was 20 times more sensitive than the later one. The novel one-step RT-PCR system was also successfully tested with field samples of turnip mosaic virus in 8 different crucifer vegetable crops.

为验证该设想,本研究建立了一步RT-PCR技术扩增体系,并利用该体系扩增和分析了国内几种蔬菜作物部分CMV分离物的2b基因序列同源性,选择特定分离物的全长2b基因,先利用PVX侵染性载体初步分析其正义和反义序列间的互作,再构建植物表达载体转化烟草和番茄,并研究转基因烟草抗CMV的表现,获得了抗CMV的转基因烟草植株。

The selection step is meticulously planned, a small step but will result in cumulative error too large.

步长的选取也是很有讲究的,过小的步长反而会导致误差累积过大。

As the opening and innovation of telecommunication market step by step, cut-throat competition has been occurred in this area in recent years.

近年来,随着电信市场的逐步开放和电信改革的深入,国内电信市场的竞争越来越激烈。

Easy step by step directions for preparing fish cutlet.

容易,一步一步指示,为准备鱼肉饼。

Fish cutlet easy step by step directions for preparing fish cutlet.

鱼肉饼容易,一步一步指示,为准备鱼肉饼。

Traditionally, when a radio network site is moved, it is necessary to interrupt existing services and perform a step-by-step serial cutover, which involves complex arrangements and huge resource investments. Therefore, there is an urgent need to discuss a low-risk and fast solution to site moving and cutover.

传统的无线网络站点搬迁,需要中断现网服务,而且只能串行逐片割接,组织工作复杂,资源投入巨大,因此亟需探讨一种低风险、快速建网的搬迁割接方案。

Traditionally, when a radio network site is moved, it is necessary to interrupt existing services and perform a step-by-step serial cutover , which involves complex arrangements and huge resource investments.

传统的无线网络站点搬迁,需要中断现网服务,而且只能串行逐片割接,组织工作复杂,资源投入巨大,因此亟需探讨一种低风险、快速建网的搬迁割接方案。

Plastic should lower 3 steps, because plastic is a step to add melt resin material to cyclinder , Higher stick of resin material ,higher screw back pressure. Screw speed differs depending on decomposing temperature of resin material ,Higher screw speed ,Higher trim force of resin, higher cyclinder temperature. Some resin material may decomposes so it set screw speed in mid-speed such as 45-75%, In plastic step setting screw back pressure is very important, Higher screw back pressure ,higher quality of product screw back pressure make resin structure order, Molding product is beautiful and volume stable.

再复杂的熔胶旋转过程最多不会超过三段,因为熔胶本身就是存在于把胶熔进料筒的过程,如果原料粘度大,熔胶压力则大,但速度则应取决于原料的分解温度,熔胶速度越快,原料中的剪切力则会越大,料管温度则越高,局部原料产生分解的可能性则会越大,故一般熔胶会采用中速为宜,如45%-75%,熔胶同时会碰到一个比较重要的环节,那就是背压的使用,产品精度要求越大,背压的使用则更大,背压可使原料分子间结构更紧密,成型出的产品则尺寸更稳定,外观越好。

Plastic should lower 3 steps, because plastic is a step to add melt resin material to cylinder , Higher stick of resin material ,higher screw back pressure. Screw speed differs depending on decomposing temperature of resin material ,Higher screw speed ,Higher trim force of resin, higher cyclinder temperature. Some resin material may decomposes so it set screw speed in mid-speed such as 45-75%, In plastic step setting screw back pressure is very important, Higher screw back pressure ,higher quality of product screw back pressure make resin structure order, Molding product is beautiful and volume stable.

再复杂的熔胶旋转过程最多不会超过三段,因为熔胶本身就是存在于把胶熔进料筒的过程,如果原料粘度大,熔胶压力则大,但速度则应取决于原料的分解温度,熔胶速度越快,原料中的剪切力则会越大,料管温度则越高,局部原料产生分解的可能性则会越大,故一般熔胶会采用中速为宜,如45%-75%,熔胶同时会碰到一个比较重要的环节,那就是背压的使用,产品精度要求越大,背压的使用则更大,背压可使原料分子间结构更紧密,成型出的产品则尺寸更稳定,外观越好。

Plastic should lower 3 steps, because plastic is a step to add melt resin material to cyclinder , Higher stick of resin material ,higher screw back pressure. Screw speed differs depending on decomposing temperature of resin material ,Higher screw speed ,Higher trim force of resin, higher cyclinder temperature.Some resin material may decomposes so it set screw speed in mid-speed such as 45-75%, In plastic step setting screw back pressure is very important, Higher screw back pressure ,higher quality of product screw back pressure make resin structure order, Molding product is beautiful and volume stable. But

再复杂的熔胶旋转过程最多不会超过三段,因为熔胶本身就是存在于把胶熔进料筒的过程,如果原料粘度大,熔胶压力则大,但速度则应取决于原料的分解温度,熔胶速度越快,原料中的剪切力则会越大,料管温度则越高,局部原料产生分解的可能性则会越大,故一般熔胶会采用中速为宜,如45%-75%,熔胶同时会碰到一个比较重要的环节,那就是背压的使用,产品精度要求越大,背压的使用则更大,背压可使原料分子间结构更紧密,成型出的产品则尺寸更稳定,外观越好。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。