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In order to reduce the non-specific binding between oligonucleotides and bases mutation caused by the complicate secondary structure of DNA and excessive PCR amplification, a frequently phenomenon in one-step gene synthesis, we used a two-step method including assembly PCR and digestion-ligation step to synthesis Aspergillus niger lipase gene lipA.

本研究主要针对一步法长片段基因合成中复杂结构的非特异性配对和过多的PCR引入碱基错配等问题，采用二步法(组装PCR和酶切-酶连)合成了黑曲霉脂肪酶基因lipA。

By the Zhangjiajie National Forest Park, Suoxi Yu nature reserves, scenic areas Tianzishan three nature reserves constitute the core of the Wulingyuan scenic spots, covering an area of 264 square kilometers, the area in 3000 Shi Feng land into the 800 streams Wan Nerita twists and turns, is melting peaks, forest, holes, lake, waterfall all in one, singular set, the show, quiet, wild, dangerous in one,"King of a five-step, a step-by-step re-ten-day", the Chinese and foreign visitors as the world No.

由张家界国家森林公园、索溪峪自然保护区、天子山自然保护区三大景区构成的武陵源核心景区，面积达264平方公里，景区内三千座石峰拨地而起，八百条溪流蜿蜒曲折，可谓融峰、林、洞、湖、瀑于一身，集奇、秀、幽、野、险于一体，&五步一个景，十步一重天&，被中外游人誉为&天下第一奇山&，&中国山水画的原本&，因而被评为全国重点风景名胜区，1982年，张家界国家森林公园成为全国第一园。

Inspired by these results, in this paper, we first give the definition of a new mapping ? uniformly Lipschitz asymptotically nonexpansive mapping on a compact subset of a uniform convex Banach space, then construct three-step iterative sequences of uniformly Lipschitz asymptotically nonexpansive mapping in this subset . We proved the convergence of this three-step iterative sequences for uniformly Lipschitz asymptoticallynonexpansive mapping, Further more, we proved this three-step iterative sequences with an error member converge to fixed points.

从中得到启发，在本文我们首先定义了一致凸Banach空间某非空紧子集上的一种新的映射——一致李普希兹渐进非扩张映射，在该紧子集上构造关于一致李普希兹渐进非扩张映射的三步迭代序列以及具误差的三步迭代序列，先来讨论三步迭代序列的收敛性，进而讨论具误差的三步迭代序列的收敛性。

The present research situation about AB process in and out of our country was analyzed in this paper. In addition to a concentrated study of the factors affecting sedimentation performance of A-step activated sludge, the rule of microbe multiplication, yield of activated sludge in A-step and treatment of low-concentration sewage were also involved in this paper. The main conclusions are as follows:(1) The study on factors affecting sedimentation performance of A-step activated sludge shown that the inflow SS distinctly improve sedimentation performance of activated sludge, the less influent SS makes the sludge bulking easy, and the pythogenous sewage evidently worsen sedimentation performance of activated sludge.

本文分析了国内外AB工艺的研究现状，对A段活性污泥沉降性能的影响因素；A段的微生物增殖规律、活性污泥产量以及A段工艺处理低浓度城市污水等问题进行了试验研究，得出了以下结论：（1）对A段活性污泥沉降性能影响因素的研究表明：进水中的SS对污泥沉降性能有明显的改善作用，如果进水中SS较少，易导致污泥膨胀；而腐化污水对活性污泥的沉降性能有显著的不良影响。

a three steps sequence therapy was designed for patients suffered post dural puncture headache total 45 cases:step one,patient keep supine recumbence,and intravenous injection of ringer's solution 1000ml with intramuscular injection of tramadol 100mg was administrated. if pdph could not relieve within 24hr,the next step should be use. step two,drinking a cup of coffee three times a day for patients who were allowed to eating or intravenous injection ketamine 0.5mg/kg for patient in fasting diet.

选择椎管内麻醉后发生头痛的病例共45例，按设定的序贯疗法分别为a：去枕平卧1d，每天补液量增加1000ml平衡液，肌注曲马多100mg，无效时进入b和c；b：能进食者给予市售咖啡饮品，每天三次；c：不能进食者静脉注射氯胺酮0.5mg/kg，连用2d；以上治疗无效时进入d：行硬膜外填充自体血10ml。

An initial sequence of steps is composed together with a following step as follows. The initial sequence of steps selects a set of nodes relative to a context node. Each node in that set is used as a context node for the following step. The sets of nodes identified by that step are unioned together.

初始定位步序列同一个作为其后续步骤的定位步结合在一起，然后初始定位步序列选择了相对于上下文节点的一集节点，最后这个集合中的每个节点被作为后续定位步的上下文节点，由后续步标识出的所有节点集被合并在一起。

As the upkeep step begins, any abilities that trigger at the beginning of that upkeep step and any abilities that triggered during the turn's untap step go on the stack.

于维持步骤开始时，在该回合之重置步骤中触发的异能，以及在维持步骤开始时触发的异能均进入堆叠。

Meng Fei and easy apartment near the restaurant in the evening to eat dinner, Chen Yese hazy in the two-step stroll with a stroll, hand in hand, talked about the beauty of the En-yu, Meng Fei leave, street lamps had been closed one after another, easy evening to see the administrator was about to close the door, then ran three steps when one step forward, until, panting as a step into the garden to the room to go.

孟菲和易夕在公寓附近的餐馆吃了晚饭，趁夜色朦胧中两人随步逛了逛，手牵着手畅谈着恩宇的美丽，孟菲离开时，路灯已陆陆续续关闭了，易夕看管理员正要关闭大门，便三步当一步向前跑去，一直到气喘吁吁的进了园子向房间迈去。

Step 4: Repeat step three with the other four bottles until you have made a six step musical scale.

步骤四：对剩下的四个瓶子重复步骤三，最后，你会得到一组六个连续音阶的组合。

Without method, oneself tried to change a map to make a tool, who knew to change had done not have weight of page of a day of content to fall completely, before snapshot of film of a lot of serialize turned N into the day, the map shows like that wait for calm, the content in the map is different nevertheless, the map that makes previously has time and address only, did not update frequency and first step, what use from the back is online generate a tool to have first step and newer frequency, because the map from the back has first step,knowing is inside the first grade with acquiescent page is reduced bring about fall authority or what are other reason, stationmaster is here people the place that ace gathers, experienced and knowledgeable, my map now is to show all the time treat calm condition, the station is Http://www.dy3166.cn, home page weight did not change, namely inside page weight was done not have, hope ace people help seek next accounts, declare, oneself are acclaimed by no means in this hair post, was to come up against this a Gordian knot really, hope ace people can help solve below, thanked.

没有办法，自己试着换了个地图生成工具，谁知道换了没过一天内容页权重全降了，好多连载影片快照都变成了N天前的，地图那一样显示待定，不过地图里的内容不一样，以前生成的地图只有时间和地址，没有更新频率和优先级，后面用的在线生成工具有优先级和更新频率，不知道是不是因为后面的地图有优先级把内页默认的优先级别降低导致的降权还是什么其他原因，这里是站长们高手云集的地方，见多识广，我的地图现在一直都是显示待定状态，站是http：//www.dy3166.cn，首页权重没有变，就是内页权重没有了，希望高手们帮忙找下原因，申明，本人在此发帖绝非炒作，是确确实实碰到了这个棘手的问题，希望高手们能够帮忙解答下，谢谢了。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。