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Human embryonic stem cell research is one of hot spots in cur re nt medical science.

人胚胎干细胞(humanembryonicstemcell,hES细胞)是当前医学研究的热点之一。

The animal models with limbal stem cell dysfunction were made by limbus region resection combined with curettement of the corneal epithelium.

以新西兰白兔为实验对象,采用角膜缘切除联合角膜上皮刮除的方法制作兔角膜缘干细胞缺乏的模型。

The animal experiment stage: 40 rabbits underwent corneal epithelium curettement and limbal ablation to induce limbal stem cell deficiency and were randomly divided into four groups, simple amniotic membrane group, non-frozen cells group, glycerol group and dimethyl sulfoxide group(n=10 for each group). Searching for the activity of frozen and non-frozen cells more completely by transplanting subculturing cells and observing the accomplishment of cells function.

动物实验阶段:将40只角膜缘干细胞损伤的模型兔随机分成单纯羊膜组、未冷冻细胞组、DMSO保存细胞组和甘油保存细胞组,每组10只,用传代培养2周后的角膜缘上皮细胞作移植实验,观察细胞体外功能完成情况来进一步比较深低温保存前后角膜缘上皮细胞的活性。

The results are as follows: the dwarf mutant A846 pertain to half-dwarf type, which height is about 38.64 cm on average, preponderating over the half-height of the wild-type, the elongation stage of the mutant represent dwarf character, internode elongation patterns of caulis are similarly the sh-type dwarf mutant, in which the first internode shows specific reduction, the elongation of these cells in the elongation zone immediately above the IM was dissimilar to the wild-type, longitudinal length of parenchyma cells were curtate, also the first internode exhibit markedly, which average is under the corresponding internode of the wild-type, the differentiation of the IM of each internodes of stem was similar to wild-type, as well as the number of internodes and the length of sheath of flag leaf.

结果表明:突变体A846平均株高38.64cm,大于野生型株高的一半,为半矮秆类型;其矮生性在拔节期显著表现;主茎茎秆各节间收缩比例不一,穗下第一节间显著缩短,与sh-型突变体类似;茎秆各节间居间分生组织细胞分化正常,由居间分生组织分化的细胞延伸受到不同程度的阻碍;各节间基本组织细胞纵向长度相应缩短,穗下第一节间缩短比例最大,其平均值小于野生型的一半;主茎节间数目与旗叶的叶鞘长类似于野生型。

Different genotypes between AA and Aa had significant difference with curvation of panicle neck, stem and leaf properties and panicle traits in erect panicle plant.

不同基因型直立穗型植株的颈穗弯曲度、茎叶性状、穗部性状都存在显著或极显著差异,进一步验证了前述直立穗性状可能是不完全显性的结论。

The stem wide and SLA and yield of small cushaw were significantly increased,too.

也能使茎秆粗壮,有效地促进单位叶面积内干物质的积累,明显提高小南瓜的产量。

This paper studies the anatomy of stem and leaf stalk of Cycas debaoensis.

对德保苏铁茎、叶柄的解剖构造进行观察,结果表明:(1)德保苏铁的茎由周皮、皮层、维管束、髓四部分构成。

Stem leaves lanceolate or linear-lanceolate; inflorescence a terminal cyme appearing leaf-opposed; seeds tuberculate.

茎生叶披针形披针形或线形;花序出现的一顶生聚伞花序对生;种子具瘤。 3 M 。 stricta 粟米草

The aim of this study was to establish the culture system of isolation and cultivation of the neural stem cells from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used.

研究采用显微解剖、无血清细胞培养和免疫荧光细胞化学染色等实验技术,成功地建立了胚胎大鼠脑和脊髓神经干细胞的分离和培养方法。

The aim of this study was to establish the culture system of isolation and cultiation of the neural stem cells from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used.

研究采用显微解剖、无血清细胞培养和免疫荧光细胞化学染色等实验技术,成功地建立了胚胎大鼠脑和脊髓神经干细胞的分离和培养方法。

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