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METHODS: Adipose was obtained from regions surrounding epididymis, retroperitoneal space and nape after anesthesis by pentobarbital, and adipose-derived mesenchymal stem cells could be harvested through type Ⅰ collagenase digestion. ADSCs were incubated in vitro and purified by differential adhesion.

戊巴比妥耳缘静脉麻醉,依次无菌取出附睾附近脂肪垫、腹膜后脂肪、颈背部脂肪,I型胶原酶酶解法分离出脂肪源间充质干细胞(adipose-derived mesenchymal stem cells,ADSCs),进行体外传代后差速贴壁培养纯化。

Human embryonic stem cells are pluripotent cells derived from inner cell mass of blastocyst at early stage of human embryo.

人胚胎干细胞(human embryonic stem cells,hES cells)是一种从人类早期胚胎的内细胞团中分离出来的多能性干细胞。

Objective To investigate the possibility of commitment differentiation of embryonic stem cells induced by the medium of cultured retinal neurons of SD rats.

目的 探讨视网膜神经细胞培养的上清液对胚胎干细胞(embryonic stem cells,ES)体外分化的诱导作用。

Objective: To investigate the mechanism of the effect of diethylstilbestrol on the proliferation and differentiation of hippocampal neural stem cells in fetal rats.

目的:探讨己烯雌酚对胎鼠海马神经干细胞(neural Stem cell, NSCs)的促增殖、分化作用及其机制。

Objectives: Being able to simultaneously express all genes of germline, endoderm, mesoderm and ectoderm, bone marrow mesenchymal stem Cells are considered to have the potential to differentiate to other tissues and cell lines.

目的 骨髓间充质干细胞(Mesenchymal Stem Cells,MSCs)能同时表达内胚层、中胚层和外胚层等所有三个胚层的基因,具有向其它细胞和组织多向分化的潜能。

PartⅠTheoretical Research MSCs is stem cells capable of multi-directional differentiation arising from mesoblast, which principally lies in stroma of connective tissues and organs, especially abundant in marrow, even divorted from fetal umbilic blood, differentiates into osteocytes, chondrocytes, tendon cells, myoctes, nerve cells, adipose cells and hematopoiesis stromal cells and so on.

理论研究间充质干细胞(mesenchymal stem cells,MSCs)是中胚层来源的具有多向分化能力的干细胞,主要存在于全身结缔组织和器官间质中,以骨髓组织中含量最为丰富,胎儿脐血中亦可分离得到。

Recently generated ESC-like pluripotent cells by reprogramming somatic cells, named induced pluripotent stem cells, open a new gate for cell transplantation-based regenerative medicine.

经体细胞重编程产生的诱导多能干细胞(induced pluripotent stem cells, iPS)也被证明有类似胚胎干细胞的特性。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞(non-hematopoietic adult stem cells,NASC)体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同,在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓,用Ficoll-hypague分离液分离出单个核细胞后,种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞(non-hematopoietic adult stem cells,NASC)体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同,在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓,用Ficoll-hypague分离液分离出单个核细胞后,种植于含10%胎牛血清的DMEM/LG培养液内。

Neural stem cells are special cells which have some capacity for self-renewal and the potencies to differentiate with multi-direction. The discovery of neural stem cells is a breakthrough for neural regeneration in the central nervous system.

神经干细胞(neural stem cells,NSCs)是一类具有自我更新能力和多向分化潜能的特殊细胞,它的发现,是中枢神经系统再生研究的一大突破。

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