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The aim of this study was to establish the culture system of isolation and cultivation of the neural stem cells from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used.

研究采用显微解剖、无血清细胞培养和免疫荧光细胞化学染色等实验技术，成功地建立了胚胎大鼠脑和脊髓神经干细胞的分离和培养方法。

The aim of this study was to establish the culture system of isolation and cultiation of the neural stem cells from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used.

研究采用显微解剖、无血清细胞培养和免疫荧光细胞化学染色等实验技术，成功地建立了胚胎大鼠脑和脊髓神经干细胞的分离和培养方法。

These culture conditions not only maintain the undifferentiated state and normal diploid karyotypes of BALB/c mouse ES cells effectively, but also maintain a series of their characteristics of murine stem cells. two different kinds of digestive methods and Two kinds of digestive juice with different concentrations were designed to dissociate proliferous inner cell mass and ES cell clonies deriver from dissociative ICM.

这一培养条件不但有效地维持了ES细胞的未分化状态和正常二倍体核型，而且维持了其作为多能性胚胎干细胞的一系列特征；实验设计了两种离散方法和两种浓度的消化液，用来离散增殖的ICM和ICM离散后出现的ES集落。

Secondly, we further isolated pluripotent stem cells from human fetal heart, liver, muscle, lung, derma, kidney, and then analyzed the characteristics and function of these cells.

在上述实验基础上，我们又相继从胎儿的心脏、肝脏、肺脏、肾脏、肌肉、真皮中分离到一干细胞亚群。

Research progress in directional differentiation from bone marrow stem cells into hepatocyte-like cells.

骨髓干细胞定向分化为肝样细胞的研究进展。

Potentially, this discovery may some day lead to transplants of stem cells from a hearing impaired individual's brain to his or her inner ear, with the cells taking on the role of hair cells.

有朝一日，这一发现可能使听力受损患者的内耳接受干细胞移植，这些干细胞来自他自己大脑中发挥毛细胞作用的干细胞。

Arrow type check valve used to be installed between drill stem and bit to prevent drilling fluid backflow from the bottom.

箭形止回阀用以安装在钻杆和钻头之间防止钻井液从井底回流。

Gene mutations were detected in 25 patients and a 18-year-old girl among 16 families. Trinucleotide repeats of CAG were 73～79. The fragments of abnormal alleles were 380～402bp, and all patients were heterozygous. The copy numbers of normal alleles were 18～40, fragments from 202～270bp. SCV reduction was much obvious compared to MCV, MCV and SCV in lower limb ?ere much more slow than that in upper's. BAEP, VEP were also delayed in latency. The anticipation in parental sex bias were much more obvioius than that in matental's. Cerebellar ataxia was most severe, the next were dysarthria and bulging eyes. Amytrophy was seen only in bed ridden patients. Cerebellar atrophy was more severe than brain stem, cord atrophy was n't observed in all MJD.

结果检出10个家系25例病人及1例症状前18岁女孩有MJD基因突变，CAG三核苷酸重复73～79次，异常等位基因片段长380～402bp，均为杂合子；正常人CAG三核苷酸重复18～40次，等位片段长200～270bp，电生理发现MJD的SCV减慢比MCV明显，而下肢的MCV、SCV又较上肢明显，BAEP、VEP均有不同程度的潜伏期延长或波的异常；MJD的父亲遗传早于母亲，进展也较块，临床以小脑性共济失调为突出症状，其次为构音障碍、突眼等，肌肉萎缩仅见于晚期病人；MRI示小脑萎缩较明显，脑干萎缩并不严重，未见明显的颈髓萎缩。

My ability to concentrate has increased up to 6 hours a day as a result of stem cell treatment, so studying is much easer than before. I no longer get tired from learning

我的精力集中能力增加到一天6小时，这是干细胞治疗的结果，我比原来更容易投入学习，学习也不感觉到累了

Cellusoft that tongue to lick my penis itch a Ma, I get, there is a sense of an immortal eclosion, came flying from stem Department pleasure.

那纤柔的舌头把的我的龟头舔得麻痒痒的，使我飘飘然，有一种羽化登仙的感觉，从茎处传来阵阵快感。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。