查询词典 stem cells

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

In the present study, we demonstrated that the mouse embryonic stem cells were differentiated into ciliated epithelial cells, with characteristics of normal ciliated cells.

通过我们的研究，实现了由小鼠胚胎干细胞向纤毛上皮细胞的分化，并且分化细胞具有正常纤毛细胞的特性。

BACKGROUND: Bone marrow mesenchymal stem cells are capable of multiple directional differentiation, and are potential cells and target cells for gene therapy.

背景：骨髓间充质干细胞具有多向分化能力，是目前极具潜力的细胞及基因治疗的靶细胞。

Potentially, this discovery may some day lead to transplants of stem cells from a hearing impaired individual's brain to his or her inner ear, with the cells taking on the role of hair cells.

有朝一日，这一发现可能使听力受损患者的内耳接受干细胞移植，这些干细胞来自他自己大脑中发挥毛细胞作用的干细胞。

Cumulative data have been documented that mesenchymal stem cells can differentiate into osteoblasts, chondrocytes, adipocytes, tenocytes, myotubes, neural-like cells, hematopoietic-supporting stroma and other non-hematopoietic cells derived from mesoblast or ectoblast.

大量研究表明，体外培养的骨髓间质干细胞在合适的诱导条件下，可分化为成骨细胞、成软骨细胞、脂肪细胞、成肌细胞、成腱细胞和神经元样细胞等多种非造血组织特别是中胚层和神经外胚层来源的组织细胞。

A couple of years ago, the research group had been already able to prove that these glia cells function as stem cells during development. This means that they are able to differentiate into functional nerve cells.

两年前，该研究小组已经证实这些胶质细胞在发育过程中具有干细胞功能，即意味着它们可分化为功能性神经细胞。

Mesenchymal stem cells are multipotent cells derived from many adult tissues, which can differentiate into cells of the mesodermal lineage, such as adipocyte, osteocyte and chondrocyte, as well as cells of other embryonic lineages. They are a promising tool for tissue engineering.

间充质干细胞是一类可从多类成体组织中分离的能向脂肪细胞、成骨细胞和软骨细胞等分化的成体干细胞，是组织工程细胞的一个理想来源。

This experiment further investigated the possibility of differentiation of BMSCs in rats into neural stem cells in vitro, and into neural cells and neuroepithelial cells, so as to provide seed cells for cell transplantation of nervous system disease.

目的：已证实骨髓基质细胞可分化为中胚层组织细胞，实验予进一步探讨体外分离培养的骨髓基质细胞向神经干细胞分化的可能性，以及是否能继续定向分化为神经细胞及神经胶质细胞，为神经系统疾病细胞移植治疗提供种子细胞。

All experiment animals lived well with no death or malignant arrhythmia following cell transplantation. Frozen sections of the heart showed a few blue-stained cells under the fluoroscope in the cell transplantation group 4 weeks later. This indicated that stem cells were successfully homing. Using hematoxylin-eosin staining, homing cells were distributed over the infarct zone, with the presence of single or mass. In the model control group, no blue-stained cells were determined.

细胞移植后两组大鼠均存活良好，未出现死亡及恶性心律失常，4周后细胞移植组心脏冰冻切片荧光镜检显示心肌内有少量胞核蓝染的细胞，提示干细胞成功归巢，结合苏木精-伊红染色，归巢细胞位于心肌梗死灶周边区域，呈单个或小团聚集；而模型对照组心肌切片未见胞核蓝染的细胞。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞，CM联合20%FBS诱导骨髓间充质干细胞，同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组，分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化，并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化，用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。