查询词典 stem cells

At present there is no effective therapeutics to treat PD except the allopathy. The discovery of neural stem cells in the adult mammalian brain provides a new chance to effect a radical cure on PD. But how to make the limited neural stem cells proliferating enough and differentiating into the dopaminergic neurons which can repair the nigrostriatal pathway and help to eliminate the PD symptoms becomes an urgent problem.

成年动物脑内神经干细胞的发现为根治帕金森病提供了新的细胞源泉，但如何使有限的神经干细胞增殖到足够数量并转化为多巴胺能神经元，使之能够修复黑质纹状体通路，逆转帕金森病临床症状，是目前亟待解决的问题。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

The 1〓 generation of which is transfusion with mature cells, such as the whole blood and blood ingredient: the representative of the second generation is hematopoietic stem/progenitor cells transplantation, which can regraft normal hematopoiesis and immunity in a body with destroyed immunity and hematopoiesis by the self-renewal and multiple differentiation capacity of stem cells.

第一代细胞疗法是成熟血细胞的输注，如全血输注及成分输血等，对于纠正各种血细胞减少有明显疗效；第二代细胞疗法的代表是干细胞移植，指利用干细胞能够在体内自我更新和多向分化的能力在病态免疫和造血功能被摧毁的机体内重建正常的造血和免疫功能。

These were raised underinducing condition to test if any morphology and structure changes. Twenty two Arabidopsismutants had various changes in morphology and anatomical structures. Twenty mutants hadlow germination rate from 10% to 60%, of which 2 had survival rates at 0% and 50%. Fivemutants occurred structure changes in the hypocotyls or stem. One of these lines, namedarris-stem, showed some unique changes: slower growth rate in comparison with the wildtype from germination to florescence; serrated margin of leaf blades, spiral rosette; morebranch in the bottom part of the stem, shorter nodes, twisted stem and branch. There were oneor several arrises along the stem. Across sections of the arrises showed one or several compactcells lumps which were round, made up of several layers of cells, looked like vascular-bundle.

在诱导条件下，这些突变体表现出不同的形态和结构的变化，共有22个表型和结构发生变化。20个突变系发芽率或存活率较低，发芽率由10％—60％，其中2个突变系存活率分别为0％和50％。5个突变系在茎或下胚轴的结构上发生变化，其中一个突变系arris-stem发生如下表型变化：从真叶出现至开花结实的整个生长期，生长速度要明显比野生型拟南芥缓慢；叶缘有明显锯齿，莲座叶呈螺旋状排列；茎的基部有较多侧枝，侧枝间距明显缩短，茎有明显扭曲；突变体茎侧面有1至数条棱形突起，内部存在一至数个排列紧密的细胞团，由多层细胞呈环形排列，细胞壁明显加厚，内部存在管状分子，推测为维管组织。

The hematopoietic system was reconstituted and the chimaeras were formed.The results showed that hematopoietic stem cells coming from ES-D3 cells could reconstitute the mice following high dose chemotherapy in vivo. This research offers theories for HSCs induced from embryonic stem cells supporting the high dose chemotherapy of cancer.

将鉴定的造血干细胞移植入大剂量化疗的C57BL/6鼠，观察造血重建功能并证明有嵌合体形成，从体内实验方面证实了ES-D3细胞来源的造血干/祖细胞移植能够重建接受大剂量化疗小鼠的造血功能，为胚胎干细胞来源的造血干细胞支持大剂量化疗提供了实验依据。

Delta gene transfer may provide a means for solving the problems of ex vivo expansion of DPSCs. The establishment of transduced DPSCs may be used as seeding cells for dental pulp regeneration or tooth engineering, and for further studies, such as the mechanisms regulating stem cells commitment and differentiation, and stem cells related gene therapy.

Delta1基因转导可能有助于解决牙髓干细胞体外培养易老化和难大规模扩增等问题；获得的基因改造牙髓干细胞一方面有望作为组织工程的种子细胞，另一方面也为进一步研究牙髓干细胞向不同方向分化机制、相关基因治疗等打下了良好的基础。

So it is generally assumed that tumor cells are derived from normal stem cells, more work is needed to identify and characterize epithelial tumor stem cells.

然而，目前研究中关于上皮肿瘤干细胞及其与正常上皮干细胞的关系了解甚少。

Adult stem cells can break away restricted lineage and be trans-differentiated into other mature cells, this phenomenon is referred to as plasticity which is the base of clinical application of adult stem cells and has a promising future.

这些干细胞可以打破胚层界限，横向分化为其他组织的成熟细胞，这种性质称为干细胞的可塑性，是成体干细胞应用于临床的理论基础，具有广阔的临床应用前景。

Sichuan cord blood stem cells Centre Ltd is mainly engaged in cord blood collection, preparation, cryopreservation, genetic testing, clinical applications and related biotechnology development and utilization of the high-tech enterprises, under the "Sichuan cord blood stem cell bank" by the Ministry of Health Approved setting, which includes the functions of the public library and the library in two parts, the public library for the vast number of patients as a source of hematopoietic stem cell cord blood, cord blood stem cell transplantation since the body can be frozen cord blood for the needs of individuals and Family members provide services.

四川省脐血干细胞中心有限公司是主要从事脐血采集、制备、冻存，基因检测、临床应用及相关生物技术开发利用的高新技术企业，下属的&四川省脐带血造血干细胞库&由卫生部批准设置，该库的功能包括公共库和自体库两部分，公共库可为广大患者提供作为造血干细胞来源的脐血，进行脐血造血干细胞移植；自体库可为需要冻存脐血的个人及家庭成员提供服务。

The quantity of CD3+ cells in the EdNSC, AdNSC of cloning goattransplantation group and control group had no statistics difference(P＞0.05) at acute and chronic period.Overall, in our study, We got the conclusions as follow: 1,The EdNSC, AdNSCof cloning goat and the AdNSC of the non-cloning goat were successful obtained;EdNSC growed faster than AdNSC in the same cell culture method. 2,The braincontusion and laceration model of cloning goats were successful established by freefalling of 50 gram of steel ball accelerate strike on the goat brain from 100cm height.3, EdNSC and AdNSC transplantation both had obvious therapeutic effect on braincontusion and laceration of cloning goats; The therapeutic effect of EdNSCtransplantation was better. 4, Allograft of stem cells has acute and chronicimmunological rejection and autografting of stem cells has no acute and chronicimmunological rejection.

在相同的条件下，EdNSC体外培养速度比成AdNSC快。2对羊脑上薛氏沟周围运动皮层区使用50g×100 cm力度作自由落体加速撞击，可产生观察指标明确且存活率高的羊脑挫裂伤模型，有望广泛用于神经干细胞移植及药物等治疗的实验研究。3胚胎神经干细胞及成体神经干细胞移植治疗脑挫裂伤后均有较明显的治疗效果；二者相比较而言胚胎神经干细胞稍好于成体神经干细胞。4中枢神经系统异体神经干细胞移植存在着明显的免疫排斥反应，而自体神经干细胞移植基本无免疫排斥反应

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。