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The prolif- erative ability of CD133 + tumor cells in vitro was estimated by MTT assay, and the proliferative ability of CD133 +, CD133 - and control SUNE cells were statistically compared. Finally, the immunocytochemical staining method and flow cytometry were used to detect differen- tial ability of CD133 + tumor cells in vitro .

采用免疫荧光细胞化学技术及流式细胞仪检测鼻咽癌细胞株SUNE中CD133的表达情况以及CD133+细胞体外分化能力;免疫磁珠分选技术纯化CD133+肿瘤细胞;MTT法检测CD133+细胞的体外增殖能力,并将其与CD133-及未分选细胞进行比较。

Supervital NBS staining Nile Blue Sulphate , a lysosomotropic vital dye which stains embryonic apoptotic, but not necrotic cell death, is employed to identify the occurence and location of apoptosis.

活体胚胎的NBS染色 NBS是一种亲溶酶体染料,可使凋亡细胞着色,而坏死细胞不着色,因而可初步判定细胞凋亡发生的区域和程度。

Methods:One hundred and twenty-nine cases of supraglottic laryngeal carcinoma were retrospectively analysed through testing nm23-H1 and laminin expression by immunohistochemical staining.

应用免疫组化染色检测nm23-H1基因和层粘连蛋白的表达,回顾性分析129例声门上喉癌的病例资料。

Using Eriochome cyanine R staining, we found that Al accumulation on surface of root tips was positive related to the inhibition of root growth in treatments of 0 - 80μmol·L~(-1) Al for 24 h. Exchangeable Al in apoplast and symplasm of root tips of wheat was effectively desorbed by 5 mmol·L~(-1) CaCl_2 at 4℃.

铬化青R染色可以原位显示0-80μmol·L~(-1)Al处理后根尖表面Al的吸附量与根尖伸长受抑制程度具有很好的一致性。5mmol·L~(-1) CaCl_2溶液可以有效洗脱10和40μmol·L~(-1)Al处理24小时后小麦根尖质外体和共质体中可交换态Al。

HE staining revealed that collagen fiber, fibroblasts and granulation tissue and synechia belt around tendons and stomas were apparently less in contrast to control group.

术后4周苏木精-伊红染色结果表明,实验组腱吻合口被胶原纤维连接,腱周及吻合口周围胶原纤维、成纤维细胞及肉芽组织明显较对照组少,腱周纤维组织较对照组粘连轻,粘连带稀少。

To detect the level of blood lipid, endodermis tetrachoric and hs-CRP.Then to observe the changes of pathomorphism with light microscope by fixing and staining to aorta pectoralis after sacrificing rats.

检测大鼠血脂、内皮四项,处死后取出胸主动脉固定、染色后,光镜下观察病理形态学改变;2。

In the ovary of pig, there were highly intensity of immunohistochemical staining of ERβ in the thecal cells of follicles.

ERβ在小鼠和猪卵巢中的表达和定位差异很大,表明ERβ在小鼠和猪卵巢中的作用也可能不同。

The results showed that the Ang II positive staining was observed not only in thecal cells , oocytes and zona pellucida of the follicles at different developmental stages but also in corpora lutea.

结果表明:Ang II分布在不同发育阶段卵泡的内、外膜细胞,卵母细胞及其透明带上,颗粒细胞和卵丘细胞未见着色。

The tinction and morphology of MGC-803 cells treated with NGF at different final concentrations of 25,50 and 75mg/ L for 24h were observed by acridine orange/ethidium bromide double staining.

4采用AO/EB双重染色法,NGF浓度分别为25,50,75mg/L,观察不同浓度NGF作用后MGC-803细胞的染色及形态变化。

Using DNA extracted by CTAB silicon-based method from leaves and endosperm of Torreya grandis, a silver staining-based AFLP system was established for leaves and endosperm respectively.

以香榧的叶片和胚乳为材料,利用改进的CTAB-硅珠吸附法提取DNA,分别就叶片与胚乳建立了香榧的AFLP银染反应体系。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。