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The HUVECs retained spindled shaped or cobblestone appearance and positively stained by anti-CD31. DAPI staining showed that 90％of scaffolds surface was reendothelialized and endothelial cells were distributed evently on the surface forming a confluent monolayer.

脱细胞支架细胞成分完全脱去，胞外基质保存完好；分离的细胞培养一周后，细胞呈梭形或多角形，核仁清晰，大小均匀，呈典型的铺路石样；免疫荧光检测可见细胞胞浆内有颗粒状红色阳性信号，说明Ⅷ因子相关抗原染色阳性；膜表面有蓝色阳性信号，说明CD31抗原染色阳性。

Then rAAV-DJ-1 was transfected into HEK-293 cells for packaging. AAV vectors encoding human DJ-1 protein were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. Tyrosine hydroxylase protein was determined using immunohistochemical staining. Ethovison software allowed measurement of movement distance in 30 minutes.

并转染HEK-293细胞，进行腺相关假病毒颗粒包装，所得病毒颗粒注射到大鼠脑内感染黑质神经细胞；4周后，注射鱼藤酮于大鼠黑质相同脑区；通过免疫组织化学方法鉴定TH蛋白表达情况，采用动物行为轨迹分析软件计算大鼠30 min内运动距离。

In normal group, the staining of FN formed intact linear structure at basement membrane and presented regular striae form in connective tissue.

正常组织基底膜中FN染色呈完整线性结构，结缔组织间质中FN染色呈规则的条纹状。

Immuno-staining results showed the nuclear signals both in BEL-7402 and HCC tissues, while GFP subcell study also support the interesting distribution pattern.

运用免疫组化双染色技术，发现TIMP-1的核内分布与PCNA呈现一定的负相关，提示TIMP-1入核的细胞可能不处于活跃增殖的状态。

Immuno-staining results showed the nuclear signals both in BEL-7402 and HCC tissues, while GFP subcell study also support the interesting distribution pattern.

通过免疫组化研究，发现在肝癌细胞BEL-7402以及肝细胞癌组织切片中，细胞核内均有阳性信号出现，而GFP的亚细胞定位也支持存在TIMP-1的核内分布现象。

Results After 3 days for operation, HE staining showed multi-focal myocardial necrosis with inflammatory cells infiltration and necrosis with more involvement in subendocardial region.

结果 术后3天，微栓塞组心肌内点状分布的坏死灶形成，多位于心内膜下，周围炎症细胞浸润明显增多，符合微栓塞病理学改变。

In control mice, mild intensity of immunoreactive staining of phosphorylated ERK1/2 was seen in the neurons of the marginal zone and the substantia gelatinosa in the caudal subnucleus of the trigeminal spinal nucleus (Sp5C).

免疫组织化学的观测表明，正常小鼠延髓尾侧亚核Ⅰ和Ⅱ层的神经元存在微弱水平的ERK1/2活性，左右侧无明显区别；尾侧亚核神经元存在高水平的磷酸化CREB。

Results: The Fos-like immunoreactive neurons distributed mainly in the spinal trigeminal subnucleus caudalisipsilaterally and medullary visceral zone (MVZ,including nucleus tractus solitarius,ventrolateral medulla and the reticular formation between the first two),ln the sections processed for double staining of Fos and TH immunohisto chemistry, numerous Fos-LI neurons showed also TH- like immunoreactivity in their cytoplasm(Fos-TH cells)in MVZ.

结果：Fos样免疫反应神经元主要分布在同侧三叉神经脊束核尾侧亚核、双侧延髓内脏带(孤束核、腹外侧区及二者之间的网状结构)。在抗Fos 和抗酪氨酸羟化酶的双重免疫染色切片上，见到延髓内脏带内许多Fos阳性神经元的胞浆同时呈TH样免疫反应(Fos-TH双重阳性细胞)。

METHODS: The breast cancer cell lines Bcap37 were treated with different concentrations of vinblatine dissolved in dimethyl sulphoxide or caspase-3 inhibitor (DEVD-CHO, 100 μmol/L) for 3 h. The changes of the proliferation were detected by MTT methods. The apoptosis was determined by observing the internucleosomal DNA cleavage and PI staining, and the proteins of pro-caspase-3 and IκΒ-α were detected by Western blotting methods.

用二甲基亚砜对照、100μmol/L caspase-3抑制剂预处理乳腺癌Bcap37细胞3h后，加入不同浓度的长春碱，以MTT法检测肿瘤细胞增殖能力，以细胞DNA片段分析及PI染色法检测肿瘤细胞凋亡，以蛋白免疫印迹法检测pro-caspase-3和IκΒ-α蛋白的变化。

Methods Immunocyto- chemical staining method and flow cytometry were used to detect the expression of putative tumor-initiated cell marker CD133 in nasopha- ryngeal carcinoma cell line SUNE, and the isolation technique with immunomagnetic beads was applied to purify CD133 + cells.

目的 检测CD133在人鼻咽癌细胞株SUNE中的表达以及CD133+肿瘤细胞的体外增殖、分化情况，并对其进行类肿瘤干细胞的鉴定。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。