查询词典 staining

Methods 24 SD rats were randomly divided into normal group,model group,aminophylline treatment group(35 mg/kg), 8 rats in each group. The bronchial asthma model in rats was built by egg protein sensibilization and longterm inhalation provocation. The rats in treatment group were lavaged each day from the first time of provocation.After 4week treatment, the rats were killed to obtain lung tissue for staining with HE.

24只SD大鼠随机分为正常组、模型组、治疗组，每组8只，除正常组外以卵蛋白致敏并吸入激发法制备大鼠哮喘模型，治疗组、模型组从第1次哮喘激发开始(造模第3周)分别给予氨茶碱、0.9%氯化钠溶液灌胃给药，1次/d，用药4周处死大鼠，取肺组织HE染色，用彩色图像分析仪测量支气管壁面积、支气管平滑肌面积，用ELISA双抗体夹心法测定肺组织MMP9和TIMP1含量。

The expression of the tumor suppressor gene PTEN was separatedly determined by RT-PCR, Western blot and immunocytochemical staining.

目的：为研究抑癌基因PTEN对胆管癌细胞生物学行为的影响，建立能稳定表达外源PTEN的胆管癌细胞系。

Methods: thirty nine full term placentae of hbsag sero positive pregnant women were collected, in which 23 were detected as hbsag positive by sabc immunohistochemistry staining.

收集血清学hbsag阳性孕妇足月胎盘组织39例，经sabc免疫组化方法在其中23例中检测到hbsag，随机选取7例采用免疫荧光双标记方法结合激光共聚焦技术检测hbsag/annexin v。

Model of animals were established by putting rats in a container with slippy inner wall and half-full of water in it, letting them swimming freely till died of being drowned. Each rat from different groups was killed by breaking its neck except drowning group, then BW and SO_2 was detected, and its chest was cut open rapidly, lung was taken out and weight was measured accurately to count lung coefficient. Its lung appearance was observed and lung tissue was fixed with formalin, which was made into pathology slices with HE staining, and pathology change of the lung tissue was observed;Expression of IL-8 and IL-10 in lung tissue was detected with Immunocytochemistry, and correlation between them was analyzed .

动物模型制作采用将大鼠放入盛有黄河水的内壁光滑的容器内，让其自由泳动自然溺死，除溺水致死组外，各组动物分别在规定的时间点断头处死，行体重和血氧饱和度等指标检测，并在冰盘中迅速开胸取肺，准确称重以计算肺系数，观察各组大鼠肺脏外观，取肺组织福尔马林固定，制成病理切片HE染色，光镜下观察肺组织病理改变；用免疫组化的方法检测肺内IL-8、IL-10的表达，并对其相关性进行分析研究。

I would cry, tears staining the ink, a smudged idea of love.

我会哭泣，泪水浸湿墨水，一个关于爱的被玷污的想法。

Double staining of isolated retina cells with anti-SRp38 and anti-Trk-C antibodies showed that SRp38 is localized in the dendrites, somata and axon terminals of rod-bipolar cells.

对分离培养的小鼠视网膜细胞进行免疫双标记分析的结果表明，SRp38蛋白在视杆-双极细胞的胞体、轴突、树突中表达。

The survival rates of CEM cells cultured with cis-diamminedichicloroplatinum added 24 h later were higher than that cultured with hTERT ASODN and DDP added 24 h later. The survival rates of CEM cells cultured with DDP were similar with that cultured with hTERT SOND and DDP. In morphological observation of apoptotic cells using Giemsa staining, cells treated with DDP or DDP combined with hTERT ASODN ro SODN at 48 h, displayed classic apoptotic changes. Apoptosis rates of CEM cells treated with DDP for 48 h after 24 h of exposure to ASODN significantly increased. There was significant difference in the percentage of apoptotic cells of CEM cells between hTERT ASODN plus DDP and SODN plus DDP or DDP alone, respectively.

结果： hTERT ASODN作用于CEM细胞24 h再加入柔红霉素、长春新碱、足叶乙甙，对细胞生长的抑制分别与单用柔红霉素、长春新碱、足叶乙甙及hTERT正义寡核苷酸联合柔红霉素、长春新碱、足叶乙甙组相比，统计学上无显著差异(P＞0.05)。hTERT反义核酸作用于CEM细胞24 h加入顺铂，再共同作用48 h，CEM活细胞均数为2.318×108 cells/L，与单用顺铂组(3.250×108 cells/L)及hTERT正义核酸联用顺铂组(3.175×108 cells/L)相比，对细胞抑制明显增强(P＜0.05)。hTERT ASODN作用于CEM细胞24 h再加入顺铂作用48 h，细胞出现典型的凋亡形态学改变。hTERT ASODN与2.5 μmol/L顺铂联合作用于CEM细胞48 h的凋亡细胞百分率(19.47%)分别同SODN与顺铂联合作用组(6.97%)、单用顺铂作用组(6.02%)进行比较有显著差异(P＜0.01)。

Methods The cultured 3T3 -L1 cells were pretreated with naringin, and then MTT method was used to detect the proliferation of the cells, oil red O staining method and spectrophotography were applied to analyze the degree of differentiation.

培养3T3-L1细胞，并用不同浓度的柚皮苷进行干预，以四甲基偶氮唑盐法检测细胞增殖，用油红O染色和染色比色法分析脂肪细胞的分化程度。

Commassie brilliant blue staining can clearly display the different structures of Sphaerotheca fuliginea, such as germinating conidia, initial germtubes, hyphae and conidiophores, and hardly stain the host tissue, presenting a striking contrast between fungus and host tissue.

考马斯亮蓝组织透明染色方法可以清楚观察到白粉病菌的5个发育阶段，即萌发的分生孢子、初生芽管、胞间菌丝、分生孢子梗和后期菌落。考马斯亮蓝几乎使寄主组织不着色，不产生背景色干扰，而菌体变深蓝色。

MLCK was distributed mainly in cytoplasm.In obstructive jaundice groups, ZO-1 and occludin staining appeared discontinuous and vague,with rough edges and spiculate processes.

梗阻性黄疸时ZO-1和Occludin分布不均，染色变淡，线条模糊，边缘粗糙有毛刺状突起；MLCK分布散乱，染色稀疏。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。