查询词典 staining

Results: Human stem cells were successfully transplanted into 6 fetal sheep and 5 lambs were born and lived. PCR analysis showed that there was human derived cell engraftment in the liver of the 5 lambs. Immunohistochemical staining and ISH with Alu probe showed human derived hepatocytes in the lobes or around periportal area, expressing albumin and CR18. Alu ISH also showed there were positive cells in the epithelia of bile duets.

结果：在移植的 6 只胎羊中，5 只顺利生产；PCR 检测显示 5 只实验羊肝脏组织中均有人源性细胞的嵌合：免疫组化及 Alu 探针原位杂交显示人源性细胞分布於门管区周围和肝小叶中，表达白蛋白、CK18 等肝细胞分化标志；Alu 探针阳胜细胞也分布於胆管上皮中。

Three hours after an administra-tion of LPS peritoneally, the number and staining intensity of positive structures were not obviously changed.

LPS免疫刺激后，阳性细胞的数量和染色强度无明显变化。

Methods The HE staining and immunohistochemistry combining with computer-assisted quantitative image analysis system were adopted to examine the mean percent area densities of stroma,epithelium and glandular lumen and the index of PCNA among periurethral zone,intermediate zone and supcapsular zone of prostate specimens from 24 patients with BPH.

应用HE染色和免疫组化染色结合计算机辅助图像分析方法，对24例BPH手术标本的三个区域中组织构成和增殖细胞核抗原表达进行定量分析。

Benzyl isothiocyanate and phenethyl isothiocyanate induced apoptosis of DU-145 cells which also were confirmed by DAPI staining and DNA gel electrophoresis.

除此之外，苯甲基异硫氰酸酯和苯乙基异硫氰酸酯也会造成人类前列腺癌细胞株DU-145粒线体膜电位的改变，活性氧化物质和钙离子的释放以及造成DNA的损伤。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine is often applied to producing bifunctional-soluble reactive dyes and is an important dye intermediate. It plays an important role in the synthesis of KN style reactive dye M style double reactive dye and modified dissimilar double reactive dye. Used 2-sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine as raw material can improve the uniformly dying performance and scourablility of dyestuff molecules and can improve the firm degree of colour staining.

2-磺酸-4-苯胺是制备水溶性双活性染料的重要中间体，在KN型活性染料、M型双活性染料以及改性异种双活性染料的合成中起着重要的作用，用2-磺酸-4-苯胺作原料合成活性染料可增加染料分子的匀染性和易洗涤性，提高染料的粘色牢度。

Osteoblasts were detected by alkaline phosphatese and calcium salt staining. The expression of osteopontin was measured by Western blotting.

成骨细胞鉴定采用组织化学方法行碱性磷酸酶和钙化斑块染色同时应用Western blotting法测定骨桥蛋白表达。

The ultrathin cryosections and ethanolic phospho tungstic acid staining sections of rat atrial cardiocytes were prepared. Quantit ative electron probe Xray microanalysis of Hall continuum method was adopted to measure the alteration of Ca content in ASG at the exact time intervals of 15, 30, 60, 120 min after the adrenaline administration.

冷冻超薄切片技术和磷钨酸-乙醇特殊染色技术制备大鼠心房切片。X射线显微定量分析采用Hall连续谱法，用以测量在肾上腺素处理后15、30、60和120 min时ASG中钙含量的变化。

A little of the diaylsed solution was dilluted with distilled water (1:4) and applied to metallic sample plate following negative staining with sodium phosphotungstate solution. The sample was freeze-dried and examined by transmission electron microscopy 1.4 IFvs size and size distribution measured 1.5 The shape morphology of IFvs 1.6 Measureing the encapsulation efficiency and loading capacity of Liposomal Gd-DTPA A sample volume of 5ml was sent to analyze (Zhongshan University Analytical Center, Guangzhou).

1.6 Gd-DTPA脂质体包封率及载药量的测定取5ml前述离心后所得上清液送交中山大学分析测试中心，采用高频电感耦合等离子发射光谱法测定离心液中Gd-DTPA含量，用加入总的Gd-DTPA量减去离心液中的Gd-DTPA含量即得包封量。

In particular, compared with conventional fluorophores, the long-lasting phosphorescence nanotubes have a broad and continuous excitaion spectrum and are photochemically stable, which can be used as fluorescent probes in biological staining and diagnostics.

对于纳米结构的长余辉材料来说，它们在纳米器件的标记和显示上具有较大的应用前景。特别是对于纳米管状结构的长余辉来说，它们具有宽的连续发射带和光化学稳定性，使得它们在生物着色和诊断上可以用做荧光探针。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。