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Methods Skp2 protein and p27 protein were examined by using immunohistochemical SP staining method in 48 cases of osteosarcoma and 16 cases of osteochondroma.

目的 探讨骨肉瘤中Skp2和p27蛋白的表达与患者临床病理和预后的关系以及两种蛋白之间的相关性。

Methods Expression of livin protein were detected by immunohistochemistry staining assay in 45 osteosarcoma cases and 30 osteochondroma cases.

采用免疫组织化学SABC法检测45例骨肉瘤、30例骨软骨瘤凋亡抑制蛋白Livin表达，并分析其与临床病理因素的关系。

Methods Skp2 protein and p27 protein were examined by using immunohistochemical SP staining method in 48 cases of osteosarcoma and 16 cases of osteochondroma.

用免疫组织化学SP法检测16例骨软骨瘤和48例骨肉瘤组织中Skp2和p27蛋白的表达情况。

The biological features of hBMSCs and cryogentic storaged cells were analyzed.hBMSCs memberane antigens were identified by immunofluorescence staining.The potential osteogenous differentiation of hBMSCs was evaluated by the osteogeneic inducer kit.

倒置光学显微镜下观察细胞形态及生长情况，细胞计数法绘制细胞生长曲线，并分析冻存细胞复苏后的生长特性，免疫荧光染色测定hBMSCs表面标志，成骨诱导试剂盒检测hBMSCs向成骨分化的潜能。

The levels of serum gonadal hormone were detected by radioimmunoassay. The effects of ZYJN on ovaries and ovarial follicular development were observed by light microscope. The expressed level of LIF was detected by using the immunohistochemical staining technique.

随机分为对照组、PCO模型组、PCO模型组＋助孕胶囊组、PCO模型组＋克罗米酚组，采用放免法测定血性激素水平的变化，光镜观察卵巢、卵泡发育的情况，免疫组化法检测卵巢组织LIF的表达水平。

Methods The PCO rat models, prepared by pregnancy hormone combine with human chorionic gonadotrophin, randomly divided into four group as control, model, Chinese crude drug and western medicine group; The levels of serum gonadal hormone were detected by radioimmunoassay; The effects of ZYC on ovaries and ovarial follicular development were observed by light microscope; And the expression level of TNF-α was detected with the immunohistochemical staining technique.

采用孕激素联合人绒毛膜促性腺激素造模法制备PCO模型大鼠。随机分为对照组、PCO模型组、PCO模型组+助孕胶囊组、PCO模型组+克罗米酚组，运用放免法测定血清性激素水平的变化；光镜观察卵巢、卵泡发育的影响，免疫组化学法检测卵巢组织肿瘤坏死因子α表达水平。

Result In the control group, oviductal mucosa and glycoprotein showed not significant changes. In the experimental group, the lesions werelimited to theoviductal mucosa. The staining result showed that CT infection decreased the secretion of the neutral glycoproteins.

结果 对照组输卵管粘膜结构和糖蛋白组分无明显变化；实验组输卵管受损部位主要局限于粘膜层，PAS Alcianblue染色显示沙眼衣原体感染可导致中性糖蛋白分泌减少。

Four weeks later, the 24 h oxaluria concentration was measured, the apoptosis index of renal tubular epithelial cells was detected with TUNEL method, and Ca2+ fluorescence intensity of cells of renal proximal tubules was determined by flow cytometry using Fluo-3/AM staining.

测定各组大鼠24 h尿草酸浓度；TUNEL技术检测肾小管上皮细胞凋亡指数；应用Flou-3/AM荧光指示剂测定大鼠肾近曲小管细胞内Ca2+的荧光强度。

Building and construction sealants - Methods of test for seepage , staining , shrinkage , shelf life and paintability

第2部分：渗流，沾污性，收缩，储存期限和可涂性的试验方法

Building and construction sealants - Methods of test for seepage , staining , shrinkage , shelf life and paintability

第2部分：渗流，沾污性，收缩，储存期限和可涂性试验方法

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。