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Objective:This study may provide neuroanatomical basis for the diagnosis and treatment of corneal disease which result in the loss of sense perception of cornea Methods:Twenty corneas of 23 week and 33 week embryo were frozen sectioned serially at 40 μm and then treated with chloride impregnation staining.

目的:为临床角膜疾病导致角膜感觉丧失的诊断和治疗的研究,提供神经解剖学依据。

Results There were three morphological types of BM metastasis of NB, microinvasive, moderately invasive and diffusely invasive pattern. CgA/NSE immunohistochemical staining was needed fur confirming the metastatic neuroblastic cells in microinvasion.

结果 神经母细胞瘤骨髓转移表现为微小浸润、中度浸润和弥漫浸润三种形式,主要根据骨髓造血组织与肿瘤成分的比例和分布而定,其中微小浸润常需行CgA/NSE免疫组化染色辅助诊断。

Results After in vivo transferring, neurocyte demonstrated strong positive signals with X-gal immunohistochemical staining.

结果:pHSVIRES-HPPE-LacZ在体感染大鼠中枢神经细胞后,用X-gal染色、RT-PCR和放射免疫检测方法均证实外源脑啡肽基因能有效表达。

RESULTS:All the vascular endothelial cells of tumors were positive for Nestin. The percentage of Nestin positive cells was 9.5%-96.2% in the neuroepithelial tumors. The positive expression rate and staining intensity of Nestin+ cells were positively correlated with the tumor pathological grades.

结果: Nestin在肿瘤内皮细胞中均有阳性染色,而在神经上皮肿瘤细胞中阳性染色比例为9.5%~96.2%,且Nestin+细胞比例及染色强度与肿瘤的恶性程度正相关。

METHODS:Nestin expression was detected in 33 cases of neuroepithelial tumors using the immunocytochemical staining. The Nestin positive and negative cells were isolated by magnetic activated cell sorting. Nestin positive cells were culutered, identified, and induced to differentiate. The expression of Nestin protein was detected in Nestin+ cells by RTPCR.

采用免疫组化法检测神经上皮肿瘤组织中Nestin的表达情况,免疫磁珠法分选肿瘤组织中的Nestin+细胞,悬浮细胞培养法进行Nestin+细胞的增殖、分化与鉴定,RTPCR法检测Nestin蛋白在其中的活性表达情况。

METHODS: The corneal endothelial cells of cats were primarily cultured, and neurone specific enolase immunocytochemistry staining was performed.

猫角膜内皮细胞原代培养,行NSE免疫组织化学染色鉴定细胞的类型、纯度及生理特性。

Cells were collected to undergo HE staining and immunocytochemistry reaction against Thy-1 to identify RGCs. Axons length of retinal neurons and RGCs were measured by microruler under microscope respectively to evaluate the neurotropic effects of B vitamins in improving the regeneration and growth of axons for retinal neurons and RGCs. Finally, we observed the reaction of retinal neurons when exposed to the insult of glucose deprivation. The neuroprotection of B vitamins were also evaluated at the same time.

对B族维生素的神经营养和保护作用的研究过程分为三个阶段,首先确定维生素B族对视网膜神经元是否有神经营养作用,并根据细胞活力找出最佳有效作用浓度;然后在接种细胞的即刻用各种维生素的最佳有效作用浓度作用于视网膜神经元,并在不同时间终止培养,收集细胞,HE染色和RGCs的免疫细胞化学染色,分别测量视网膜神经元和RGCs的轴突长度,评价维生素B族是否有促进视网膜神经元和RGCs轴突再生伸长的神经营养作用;最后,观察视网膜神经元对低糖损伤的反应,及维生素B族对受损的细胞是否有保护作用。

At the depigmentation assay, DH and EH show dose-dependently inhibitory activities against mushroom tyrosinase, and the IC50 is 5.697 and 5.256 mM, respectively, and show noncompetitively inhibitory modes in the present of tyrosine, but not DOPA. The control synthetic products, D and E, have no inhibitory effects on tyrosinase. Using tyrosinase activity staining in PAGE gels, both DH and EH show dose-dependently inhibitory pattern in the presence of tyrosine, while, EH also could suppress tyrosinase activity in the presence of DOPA.

而美白活性方面,以商品酪胺酸以商品酪胺酸酵素进行实验,当基质为酪胺酸时,发现DH与EH具浓度相关抑制效果,IC50分别为5.697与5.256 mM,且其相对於基质酪胺酸之抑制型态皆为非竞争型抑制,而其结构类似物之对照化合物D与E则不具此活性;但当基质为多巴时则皆不具此活性;利用原态PAGE电泳活性染色亦发现抑制酪胺酸酵素结果,但EH在基质为多巴时也能下降酪胺酸酵素活性。

Nonfermentative bacilli growing on solid medium and enterobacteria growing in flagella broth were more suitable for flagella-staining.

非发酵菌适合于固体培养进行鞭毛染色获得最佳效果,液体培养对于一些肠杆菌鞭毛染色更为适合。

Histomorphometric studies were done to measure the following indexes: the IOD values of pulmonary, skeletal muscular and pleural small vascular walls from staining of AT1 and AT2 receptors, the pulmonary vascular density, muscularization of the nonmuscular vessels, percent wall thickness and proliferation of pulmonary small vascular walls.

结果:先心病患儿肺小血管壁AT1、AT2受体染色的IOD值均高于对照组(P=0.000);先心病患儿骨骼肌和胸膜小血管壁AT1、AT2受体染色的IOD值均高于肺小血管(P<0.05);先心病患儿肺小血管壁AT1受体染色的IOD值与肺血管密度、非肌性肺血管肌化程度、肺小血管壁厚百分比和肺小血管壁细胞的增殖度相关(r分别为-0.783、0.742、0.521、0.657),AT2受体仅与肺血管密度和血管壁厚百分比相关(r分别为-0.487、0.516)。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

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