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Results Six days after culture, a large number of multinuclear mature osteoclasts were formed, and TRAP staining confirmed them to be mature osteoclasts.

结果 混合培养6d后，体系中可见成熟破骨细胞出现。

Results High homogenous hMSCs were obtained and after co-culture for 5 d with osteoblasts, they fused to form multinuclear cell clusters in micrograph and were postive in TRAP staining. Conclusion The co-culture system method is successful.

结果：体外分离、培养的hMSCs具有独特的细胞免疫学表型，即CD73和CD105阳性， CD34和CD45 阴性。hMSCs与成骨细胞共同培养5 d后，即可见单个核细胞融合成多核细胞，TRAP 染色可见多数细胞胞浆呈酒红色，为诱导成功的破骨细胞。

Methods Marrow cells from NH mouse were harvested and cultured in α-MEM with 10% fetal bovine serum.The appearance of tartrate-resistant acid phosphatase-staining multinuclear cells and formation of bone resorption pits were measured after 3,6,9 and 12 day exposure to various concentrations of 1,25-2D3 in culture. Results 1,25-2D3 (concentration higher than 10-9mol/L) could induce recruitment of OLCs and their bone resorption activity in a dose-dependent manner.

收集NH小鼠骨髓细胞于含10%胎牛血清的α-MEM培养基中行体外培养，设置不同的1，25-2D3浓度组和给药时间组，并于培养第3、6、9、12天观察记录抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase，TRAP)阳性多核巨细胞［或破骨细胞样细胞(osteroblast-like cell，OLC)］以及骨磨片上骨吸收陷窝的数目。

Three-hundred and ninety-nine fungal isolates were obtained from 686 diseased cotton seedlings and soil samples collected from cotton production areas in Northern Xinjiang by using the conventional method,among which 272 strains of R.solani were identified.Based on Giemsa staining,all strains were identified as multinucleate Rhizoctonia solani .

从新疆北疆棉区采集了典型的棉花立枯病病苗及棉田土标样686份，按常规分离方法分离得到399个分离物，从中鉴定出272个纯化的立枯丝核菌( Rhizoctonia solani Kühn)菌株。

The results showed that the cells had classic characteristics of osteoclasts: multinucleated giant cells, staining positively for TRAP in cells, forming bone absorptive lacunae on the bone slices.

结果表明，直接分离的细胞具有典型的破骨细胞的形态特点：属多核大细胞，TRAP染色阳性，能在骨片表面形成吸收陷窝。

Ache staining showed that in the end-to-side neurorrhaphy group the percentage of motor fibers of musculocutaneous nerve main branch was 0.39±0.07, not different from normal control group.

周围神经端侧缝合后有相当数目的再生神经纤维，但以髓鞘薄，直径小的纤维为主，同时能有效防止肌肉萎缩。

METHODS: Thirty male SD rats were randomly divided into control group, stress group and NG-nitro-L-arginine methyl ester group. The rat model of water immersion-restraint stress was established. The expression of nNOS in colonic submucous plexus and myenteric plexus in the rats was examined by immuno-histochemical staining and analyzed by computer image analysis system.

SD大鼠30只随机分为对照组，应激组和L-NAME组，采用水浸-束缚应激动物模型，用免疫组织化学ABC法检测nNOS在大鼠结肠黏膜下神经丛和肌间神经丛的表达，应用计算机图像分析系统对其表达进行定量分析。

Others injected MSCs into autoallergic myocardial tissue directly, observed some caridomyocyto-like cells formation capable of cardiomyocyte specific marking of troponin T and myoglobulin heavy-chain positive staining, which proved that MSCs can be induced into myocardial cells in microenviroment in vivo.

另有学者将MSCs直接注入自体心肌组织内，发现有心肌样细胞形成，具有肌钙蛋白T和肌球蛋白重链染色阳性的心肌细胞特异性标志，表明MSCs在体内微环境条件下可以诱导分化为心肌细胞。

Results The expression of the Mahoganoid protein and its mRNA was showed by brown staining and observed in the Leydig cells, spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and peritubular myoid cells of testicular tissues and principal cells, basic cells and epithelial cells of epididymal tissues.

结果1。免疫组化法检测Mahoganoid蛋白的实验结果显示的棕色阳性信号可见于各天龄段大鼠睾丸的生精细胞、支持细胞、间质细胞和管周肌样细胞以及附睾输出管的高柱状、低柱状上皮细胞和附睾管的上皮细胞、主细胞、基细胞。其中Mahoganoid蛋白主要表达于胞膜和胞浆。

3Rhe distribution and change of proliferating cell nuclear antigen in seminiferous tubule: The positive staining of proliferating cell nuclear antigen was found in slices of all groups, the immunological positive products were located at karyon, usually in brown yellow granules, only a small part of them in diffuse state; the immunological positive cells were mainly the spermospores, besides a small quantity were spermiocyte, supporting the cell and myoid cell nuclear to be stained.

3增殖细胞核抗原在生精小管中的分布及变化：各组切片均可见增殖细胞核抗原阳性染色，免疫阳性产物位于细胞核，通常呈棕黄色颗粒，小部分呈弥散状；免疫阳性细胞主要是精原细胞，另外还有少量初级精母细胞、支持细胞和肌样细胞核着色。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。