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Bone marrow-derived mesenchymal stem cells are capable of chondrogenesis, making them a possible source of cells for injectable cartilage tissue engineering. There exist different ideas on the ability of mesenchymal stem cells's chondrogenesis in monolayer culture. Because of this, the effect of adult rabbit's bone marrow-derived mesenchymal stem cells chondrogenesis in monolayer culture was studied. The mesenchymal stem cells was isolated from adult rabbit's bone marrow and monolayer cultured. TGF-β1, Vit-C and Dexamethasone were used. Immunohistochemistry analyses and histological staining of H-E, Methylaniline blue and Alcian blue were performed to identify the expression of collagen type Ⅱ and cartilage associated matrix. The results showed that the induced cells expressed and produced collagen type Ⅱ and cartilage associated matrix. This suggests that the differentiation of adult rabbit's marrow-derived mesenchymal stem cells into chondrocyte in monolayer culture is feasible and may be induced by TGF-β1, Vit-C and Dexamethasone.

骨髓基质干细胞的软骨分化潜能使其可能成为可注射组织工程化软骨研究的种子细胞，为探讨体外培养的骨髓基质干细胞在平面诱导条件下软骨分化的可行性，我们进行下列实验：获取并体外平面培养成体兔骨髓基质干细胞，应用TGF-β〓、Vit-C和地塞米松对其软骨分化诱导，诱导后的骨髓基质干细胞行细胞爬片组织学和Ⅱ型胶原免疫组化，结果证实，诱导后骨髓基质干细胞可分泌Ⅱ型胶原，组织学染色可见类似于软骨细胞，由此证明体外培养的骨髓基质干细胞在平面诱导条件下可以软骨分化，其软骨诱导因子为TGF-β〓、Vit-C和地塞米松。

Counting method of microorganisms in environment;②preparation of smears for observing forms and structures of bacteria、Actinomyces、yeasts and molds, methods and technologies of staining and oil immersion objective;③cell counting techniques and microscope examination techniques;④preparation of microbic media and sterilization techniques;⑤aseptic operations and microbic inoculation methods ;⑥technics of microbic separation、purification and cultivation;⑦physiological and biochemical reactions of microorganisms and the microbial examination techniques of food sanitation to strengthen students the operation techniques.

课程内容：本课程实验内容包括：①环境中微生物检测计数；②细菌、放线菌、酵母及霉菌细胞形态、结构的制片，染色和显微镜观测，油浸物镜的使用技术；③细胞计数技术和显微测量技术；④微生物培养基制备、压力蒸气灭菌技术；⑤无菌操作技术、移种技术；⑥微生物分离、纯化和培养技术；⑦微生物的生理、生化反应及食品卫生微生物检测技术等旨在加强学生微生物学的操作技术。

In order to provide a reference for controlling microsporidian infection effectively, this paper reviews the progress of studying on the detecting technology from the microscopic staining methods, immunological and molecular biology.

本文简要综述了以染色技术、免疫组化技术和分子生物学技术等为主的微孢子虫识别方法的研究进展，以期为有效防治微孢子虫病提供参考。

Methods The polysaccharide was extracted and purified from Millettia Speciosa Champ,CFDA-SE staining and flow cytometry were used to detect the effects of polysaccharide on mouse lymphocyte proliferation stimulated by Con A.

提取、纯化牛大力多糖，利用CFDA-SE染色，流式细胞术检测多糖对Con A刺激引起的小鼠T淋巴细胞增殖的影响。

Methods The polysaccharide was extracted and purified from Millettia Speciosa Champ,CFDA-SE staining and flow cytometry were used to detect the effects of polysaccharide on mouse lymphocyte proliferation stimulated by Con A.

提取纯化多糖，流式细胞术检测多糖对刀豆蛋白A刺激引起的小鼠T淋巴细胞增殖的影响，探讨牛大力多糖对免疫系统的调节作用机制，为临床用牛大力治疗多种慢性疾病提供理论和实验依据。

The following examining methods were carried out:(1)Phagocytosis of Monilia albicans by the peritoneal macrophages by means of fluorescence microscopy after acridine orange staining.(2)Measurement of non-specific esterase in Mφ after being stained with α-nephthy acetate by image analysis.

激光照射结束后取小鼠腹腔巨噬细胞，分别做如下实验：(1)用荧光显微镜观察腹腔巨噬细胞对白色念珠菌的吞噬功能；(2)用α-荼酚醋酸酯法显示腹腔巨噬细胞内非特异性酯酶，并用图像分析系统对NSE作定量分析；(3)用透射电镜观察腹腔巨噬细胞超微结构的变化。

Mononucleated trophoblastic cells were variably positive for SALL4 staining in all 5 choriocarcinomas.

所有ITGCNs、经典型精原细胞瘤和ECs病例90%以上的瘤细胞强烈表达SALL4和OCT4。

The isonucleus-allocytoplasmic materials ms-Xinong 1376,-Xinong 1376, and ms-Xinong 1376 were used to investigate the protein expression profile in mononucleated anaphase anthers of wheat (Triticum aestivum L.) after silver staining and analysis by PDQuest software. About 320 to 350 distinct protein spots were detected reproducibly.

为了研究小麦雄性不育蛋白表达机制，以具有异质同核的3个亲本：即遗传型不育系ms-西农1376、对应的保持系-西农1376和生理型(化学杀雄剂SQ-1诱导)雄性不育系ms-西农1376为材料，利用IEF/SDS-PAGE凝胶电泳技术，对发育到单核后期的花药全蛋白特异性进行了比较分析，得到320~350个清晰的蛋白点。

In addition of cytomorphologic identification, immunolhistochemic technique of bone morphogenic protein-monoclonal antibody, alkaline Phosphatase staining and concentration assays, as well as osteoblasts reaction to PTH-cAMP were used, according to the biologic character of osteoblasts.

除了在细胞形态学上对其鉴定外，还根据成骨细胞的生物学特性，首次引用了骨形成蛋白单克隆抗体免疫细胞化学技术，以及碱性磷酸酶染色和含量测定，成骨细胞对甲状旁腺激素-cAMP反应等方法，验证了经酶消化分离培养的兔颅骨细胞具有成骨细胞的功能和特性。

Hybridization of cDNA probe and gene chip was carried out, gene chip was scanned with Scan Array3000, and results were analyzed with ImaGene 3. 0. Result: 1 Toluidine blue staining of semithin section showed: compared with control group, the boundary of tunica intima, tunica media and tunica adventitia was not distinct, tunica intima and internal elastic membrane was thickened and multilaminar.

将标记了荧光素的cDNA与细胞周期相关基因表达谱芯片杂交，扫描并分析杂交结果。cy5/cy3 ratio值在0.5～2.O之外且一对Double基因表达趋势一致的基因为表达有差异基因，ratio值＞2.O表明DM组该基因上调，ratio值＜0.5表明DM组该基因下调。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。