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High iron diamine/alcian blue staining and Hematoxylin and Eosin staining was used to classify IM and gastric carcinoma, and the expression of COX-2 protein detected in different subtypes of IM and gastric cancer by using immunohistochemistry.

分别用高铁二铵／爱先蓝及HE染色对IM及胃癌进行分型，免疫组化检测不同亚型IM及胃癌中COX一2蛋白的表达。

After 20 d calcium induction, jacinth calcium salts could be detected in the cells by alizarin red staining.At day 10 after being adipoinduced, the cells showed positive oil red O staining.

结果：成功获得了稳定表达GFP的ADSCs，GFPADSC经成骨诱导20 d后茜素红染色可见有橘红色钙盐沉积；经成脂诱导10 d后油红O染色阳性。

After 20 d calcium induction, jacinth calcium salts could be detected in the cells by alizarin red staining.At day 10 after being adipo induced, the cells showed positive oil red O staining.

结果：成功获得了稳定表达GFP的ADSCs，GFP ADSC经成骨诱导20 d后茜素红染色可见有橘红色钙盐沉积；经成脂诱导10 d后油红O染色阳性。

The adipoinduced MSCs were detected with oil red O staining. RESULTS: ADSCs, stably expressing GFP, were obtained successfully from the transgenic mice with GFP gene. After 20 d calcium induction, jacinth calcium salts could be detected in the cells by alizarin red staining.

结果：成功获得了稳定表达GFP的ADSCs，GFPADSC经成骨诱导20 d后茜素红染色可见有橘红色钙盐沉积；经成脂诱导10 d后油红O染色阳性。

Methods 2 cases of sudden death lying-in women with ammiotic fluid embolism were analyzed using HE staining and S-P immunohistochemical staining.

分析2例羊水栓塞所致产妇猝死病例的尸检HE切片，并进行免疫组化标记观察。

The freezing technology of papanicolaou test is one meliorative technique based on papanicolaou test, the technique emphasizes on every process of sampling,smearing and staining, the most important step is the fixed smears should be soaked into the restoring-liquid of cells morphology based on the theory of penetration and expansion principle,thus achieve the purpose to cells restoring their normal appearance,to promoting cells staining and to improving cells transparency and clarity, it will be favorable to improve the cells recognition rate,the positive rate,the specification,sensitivity and decrease leaking rate and misdiagnosis rate.

冷冻式巴氏涂片技术是建立在传统巴氏涂片基础上的一种改良技术，该法在制片的过程中强调注重取材制片的全过程，其核心步骤是把固定好后的细胞玻片置入细胞形态修复液中，利用渗透膨胀的原理对已干枯萎的细胞进行修复，从而达到恢复细胞形态的目的，促进细胞着色，提高细胞透明度和清晰度，有利于对病变细胞的识别和提高对宫颈病变细胞的阳性检出率、特异度和灵敏度，降低漏诊误诊率。

Methods: Twenty-four Kkay mice were divided into three groups(n=8):diabetes group,methionine-diet group,folic acid and vitamin B12 treatment group. C57Bl/6 mice were used as controls(C57). Four months later,the mice were sacrificed and their serum levels of Hcy,folic acid and vitamin B12 were measured by fluorescence polarization immunoassay. Pathological changes in kidneys were examined with the periodic acid schiff method with periodic acid-silver methenamine staining under electron microscope. Expression of MMP-9 protein or mRNA was detected by RT-PCR with immunohistochemical staining. Results: Different morphological changes of DN were found in Kkay mice.

Kkay糖尿病鼠随机分3组：糖尿病组、高蛋氨酸组和叶酸、维生素B12组，C57BL/6鼠为对照(C57)，以相应饲料喂养4个月后取材，测定血糖、血同型半胱氨酸、叶酸和维生素B12，以PAS、六氨银染色法光镜下及超微电镜观察各组DN病变，比较各组肾小球硬化指数；以免疫组化和半定量图像分析肾小球MMP-9蛋白表达，并以RT-PCR检测肾脏MMP-9 mRNA水平。

Pneumocystis carinii cysts were observed in the lung print smear after staining with gormori methenamine silver,and pathological changes in the lung section were observed after staining with hematoxylin-eosin.

3肺组织印片六亚甲基四胺银染色观察卡氏肺孢子虫(Pneumocystis carinii，Pc)包囊，肺组织切片苏木素-伊红(hematoxylin-eosin，HE)染色观察其病理变化。

Rat AAA model was made by perfusion PPE elastase through the abdominal aorta, and the specimen was obtained on postoperativeday 3, 7, 14, 28, respectively. Specific elastic fiber staining was used to observe the disruption of elastic fiber in aortic wall. Immunohistochemistry staining of CD68 was applied to observe the microphage infiltration. The in tisu hybridization and western blot of MCP-1 and MMP-2 were used to study the expression of mRNA and protein. Thus we can analyze the facilitated function of MCP-1 gene to the microphage adherence and infiltration on the earlier AAA.

采用经腹主动脉腔内导管灌注的方法制成大鼠AAA模型，分别于术后3天、1周、2周、3周、4周获取各组大鼠腹主动脉标本，行弹力纤维特殊染色观察动脉壁弹力纤维的破坏情况；行CD68免疫组织化学染色观察动脉壁中巨噬细胞的浸润；行MCP-1及MMP-2的原位分子杂交、Western蛋白质印迹观察二者的mRNA表达和蛋白表达；分析MCP-1基因促进巨噬细胞的黏附、浸润在早期AAA发病中的作用。

RESULTS: A total of 36 rabbit models, 6 in the 1-month therapy group and 8 in the 3-month therapy group were died from infection. In the 1-month therapy group, thick ureteric wall and smooth ureteric lumina developed without any contracture. Under the optical microscope, transitional epithelial cells covered throughout the inner walls and smooth muscle cells appeared sparsely in a mussy configuration. In addition, CKAE1/AE3 staining was positive butα-actin staining was very weak when checked by immunohistochemistry. In the 3-month therapy group, amnion-ureters were enclosed by their peripheral tissue and showed rich blood vessels and normal ureteric lumina without significant contracture, futher more, there had formed a well-arranged transitional epithelial layer and smooth muscular layer, the immunohistochemistry result showed that the expression of CKAE1/AE3 and α-actin were positive.

结果：36只大白兔中，1个月治疗组死亡6只，3个月治疗组死亡8只，死亡原因均为感染。1个月治疗组大白兔羊膜输尿管柔软，长4 cm，管壁变厚，无挛缩，移行上皮组织爬满内壁，平滑肌组织部分再生，但较稀疏，排列不规则；免疫组织化学检查提示CKAE1/AE3染色阳性，α-Actin染色微弱。3个月治疗组大白兔羊膜输尿管与周围粘连，有丰富的血运，无明显挛缩，管腔不缩小，已形成良好的移行上皮层和平滑肌层，免疫组织化学显示α-Actin和CKAE1/AE3染色均为阳性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。