查询词典 staining

Methods: Thirty male SpragueDawley rats were randomly divided into 5 groups, group A 3 days after L5 VRT, group B 7 days, group C 14 days, group D 21 days and group E of sham operation, whose left spine cords were exposed by hemilaminectomy. Rats in each group were respectively perfused with paraformaldehyde and materials were taken from L5 dosal root ganglions and the changes of expression of P2X3 receptor were observed with immunofluorescence staining.

雄性SD大鼠30只，随机分为5组（n=6），A组（L5 VRT术后3 d）、B组（L5 VRT术后7 d）、C组（L5 VRT术后14 d）、D组（L5 VRT术后21 d）模型组和E组，E组仅行半椎板切除术暴露左侧脊髓，分别在各自的时间点灌注取材L5背根神经节，用免疫荧光染色方法观察P2X3受体表达的变化。

Results indicated that suppression of malignancy was observed in the hybrid cells, they became differentiated morphologically and functionally by:(1) changes in histochemical staining, nucleus-cytoplasm in ratio, and increase in cell size and nucleal heterochromation, but decrese in cell surface microvilli and number of nucleoli;(2) decrease of growth rate, mitotic index and DNA synthesis rate;(3) loss of the ability to growth in soft agar medium, and non-tumorigenic under heterotransplantation to nude mice;(4) reduction of chromosome number mainly of rat derivative and decrease in chromosomal aberration rate.

实验结果表明：（1）杂交细胞出现瘤细胞与网织红细胞杂交同样的表型逆转及去恶性化特征，主要表现在细胞体积增大、核质比例减小；DNA合同速率降低、分裂指数下降，细胞生长显著减慢；在软琼脂中不形成集落，在裸鼠体内失去了长瘤能力。

Methods The specimens which were identified as osteosarcoma were cultured primarily in vitro. The surviving cells underwent morphological observation, histochemical staining, isoenzyme pedigree determination, cell cycling analysis, karyotype analysis and heterotransplantation.

采用原代组织块培养法对成骨肉瘤手术标本进行培养，对存活细胞进行形态学观察、组织化学染色、乳酸脱氢酶同工酶谱、细胞周期检查、核型分析、异种移植。

Results:Visible black spots come into being in subcutaneous layer of experimental animal.There is notable difference in skin histiocytic structure between tsst of skin becoming black spots and control group,by way of skin tissue slice staining and observatiopn with optical and transmission electron microscope.

结果：受试动物皮下可形成肉眼可见的黑斑，通过皮肤组织切片染色并用光镜和透射电镜观察，黑斑形成处皮肤组织细胞学结构与正常对照组有明显区别。

As shown,the antigen is a glycoprotein present on the surface of cells. Extracts from Hep G2 hepatocellular carcinoma cells, HT-29 colon cancer cells, SKHep-1 liver adenocarcinoma cells, BT-20 breast cancer cells as well as U937 histiocytic lymphoma cells were positive for the precipitation of Ag-Ab complex, which were later reactive with the anti-3A5 McAb staining. While extracts from normal human endothelial cells showed no precipitation in Western blot.

抗原分子量为63kDa，是一种存在于细胞膜上的糖蛋白，在肝细胞肝癌Hep G2细胞，肠癌HT-29细胞，肝管癌SK-Hep-1细胞，乳腺癌BT-20和人组织细胞淋巴瘤U937细胞膜提取物中，均有此蛋白带，并能被单抗3A5特异性地识别，而人正常内皮细胞则不见此反应带。

Histochemical staining showed the temporary expression rate of GUS gene reached more than 70%.

组织化学染色结果表明：GUS基因的瞬时表达率可以达到70%以上。

Methods The study was carried out by using the common and special histochemical staining.

用组织化学和特殊组织化学染色观察。

Histochemical staining test showed the temporaryexpression rate of GUS gene reached more than 70%.

组织化学检测表明：GUS基因的瞬间表达率可以达到70％以上。

Methods The clinical data of the 20 cases were collected and muscle biopsies were done with routine and histochemical staining.

目的 从临床和肌肉病理的角度分析脂质沉积性肌病的特点。

Methods light microscopy、histochemical and immunohistochemical staining were used with the review of the literature.

对1例乳腺分泌型癌进行光镜、组织化学和免疫组化观察并结合文献进行讨论。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。