查询词典 staining

Methods:SP immunohistochemistry staining was applied to detect the expression of p130Cas and paxillin in tumor tissues from 53 cases of primary breast carcinoma, 10 cases of breast fibroadenoma and in 10 cases of normal breast tissues.

采用免疫组化SP法检测53例原发乳腺癌，10例乳腺纤维腺瘤，10例正常乳腺组织中p130Cas和paxillin蛋白的表达情况。

Methods Nuclear β-catenin expression was detected by immunohistochemistry in 77 lesions with desmoid-type fibromatosis and 171 other spindle cell lesions, including superficial fibrmoatosis (n=18), nodular fasciitis (n=36), keloid (n=16), scar (n=10), granulation tissue (n=9), synovial sarcoma (n=38), neufibroma (n=13), solitary fibrous tumor (n=12), gastrointestinal stromal tumor (n=10), low-grade myxofibrosarcoma (n=3), low-grade fibromyxoid sarcoma (n=3), and smooth muscle tumor (n=10). In addition, the immunohistochemical expressions of ER-α, ER-β and Ki-67 were examined in all of the lesions with desmoid-type fibromatosis. The nuclear immunohistochemical staining for nuclear βcatenin and ER-β was graded as high level (≥25% of cells), low level (5%-25%) or none.

采用免疫组织化学染色法检测77例韧带样型纤维瘤病和171例其它良、恶性梭形细胞病变（包括结节性筋膜炎36例、浅部纤维瘤病11例、瘢痕疙瘩16例、增生性瘢痕10例、肉芽组织9例、滑膜肉瘤38例、神经纤维瘤13例、孤立性纤维性肿瘤12例、胃肠间质肿瘤10例、低度恶性黏液纤维肉瘤3例、低度恶性纤维黏液样肉瘤3例及平滑肌肿瘤10例）组织中β-catenin核阳性的表达，同时检测韧带样型纤维瘤病中ER-α、ER-β和Ki-67的表达。

High-level nuclear β-catenin staining serves as a useful diagnostic tool for desmoid-type fibromatosis. The high expression of ER-β in desmoid-type fibromatosis provides a biological mechanism for the antiestrogenic compounds to treat fibromatosis.

高水平β-catenin核阳性表达可用于辅助韧带样型纤维瘤病诊断以及与其它梭形细胞肿瘤的鉴别诊断。

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞，建立小鼠T淋巴细胞活化、增殖的模型，以不同浓度的CPT作用于该模型，流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以Con A作为T细胞活化、增殖的刺激剂，以不同浓度的TMB-8及与环孢菌素A联合作用于该系统，用流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术，分析TMB-8在Con A刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色分析细胞周期的分布情况。

The proliferation and kinetics, cell cycle, clonalforming rate, and apoptosis of MEC- 1 cells, before and after FHIT gene transfection in vitro, and tumor loci formedon mice after injection of transferred MEC- 1 cells in vivo were observed by immunochemical staining, flow cytometryanalysis, and so on.

结果外源性FHIT基因转入黏液表皮样癌MEC- 1细胞后，S期细胞显著减少，G1期细胞增多；细胞倍增时间从（21.03±0.41）h延长为（26.86±0.71）h；软琼脂克隆形成率显著降低；细胞发生退行性改变和出现凋亡早期表现。

The cortico- fugal fibers terminating into cochlear nuclei were traced in sections by means of the modified Nauta and Fink-Heimer silver staining method.

毁伤一侧大脑皮质听区或躯体感觉Ⅰ区后，在双侧耳蜗神经核中出现变性纤维，证明上述皮质区与耳蜗神经核闯有直接的下行纤维联系。2。

Results By using zinc-iodide-osmium staining, the tubulo-vesicular system of parietal cells of fundic gland was manifested as numerous fibrous structures under HVEM. The nematolysosomes was often demonstrated as branching in shape and anastomosed with each other making a network system in cytoplasm under the visual field of the three dimensional electron microscopy combined with AcPase cytochemistry.

结果高压电镜下显示，锌-碘-锇酸染色方法制备的标本胃底腺壁细胞的微管泡系统呈无数纤维状结构；AcPase结合三维电子显微镜观察显示，线性线粒体在胞质中常呈分支状并互相吻合成网。

Methods: animals were randomly classified into 5 groups: group a, gaster poured by physiological solution; group b, gaster poured by ethanol; group c, gaster poured by ethanol after hypophrenic vagectomy; group d, gaster poured by ethanol after big small splanchnicotomy; group e, gaster poured by ethanol after hypophrenic vagotomy and big small splanchnicotomy.the distribution of c fos positive neuron in central nervous system was displayed and immunohistochemical staining method was used.

取30只成年雄性wistar大鼠，随机分为5组，每组6只。a组：单纯生理盐水灌胃组；b组：单纯乙醇灌胃组；c组：膈下迷走神经切断乙醇灌胃组；d组：内脏大、小神经切断乙醇灌胃组；e组：膈下迷走神经和内脏大、小神经均切断乙醇灌胃组。运用免疫组化技术显示fos阳性神经元在大鼠中枢神经系统中的分布。

What was observed under a light microscope included: tumor cells were mulberry and micropapillaryshaped or it was of glandule tubular arrangement; there was obvious interspace between cancer nest and neighboring areas; micropapillary was empty of fiber blood vessel axes; immunohistochemical staining showed EMA positive location was both at outward surface of glandule duct and at micropapillarylike cancer nest.

光镜下特征性表现为肿瘤细胞呈桑椹状、微乳头状或小腺管样排列，癌巢与周围间质形成明显的空隙。微乳头缺乏纤维血管轴心。免疫组化染色EMA阳性部位在癌细胞巢团或微乳头状、腺管的外表面。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。