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Staining and observing shows that the most pathological changes were interstitial pneumonia and hemorrhagic pneumonia, typical nonsuppurative encephalitis, and neurogangliitis et al. The lymphoid tissue was necrosis, and the amount of lymphocytes was reduced. The pathological changes of the positive cells, especially the epithelial cells were swell, denaturization and hyperplasy to the extent.
H.E。染色观察显示,病理组织学变化主要表现为间质性肺炎和出血性肺炎、典型的非化脓性脑炎、神经节炎等;淋巴结、扁桃体和脾脏等组织内淋巴细胞坏死、数目减少;有阳性反应的细胞,特别是阳性上皮细胞呈现不同程度的肿胀、变性、增生等病理变化。
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Results: After 24h exposure to rhBMP-2, the expression of PCNA in HFCL cells decreased 0.65 fold by densimetric scanning. Observed after staining with the Wright-Gimsa, the numbers of cells with mitosis imaging decreased, but the morphologic change of cells did not happen. The HFCL cell numbers in S phsae decreased while in G1 phase increased, the cell cycle was arrested at G1 phase HFCL cells did not differentiate to myelocytic or mononucleate cells.
结果:经过rhBMP-2作用24h后,HFCL细胞PCNA表达下降,经灰度扫描下降幅度为0.65倍;瑞氏-吉姆萨染色后发现细胞核分裂像减少,分裂指数下降,但细胞形态没有变化;HFCL细胞S期中的细胞数量减少,G1期数量增多,出现G1期阻滞;rhBMP-2作用没有使HFCL细胞向髓系或单核方向分化的趋势。
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The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.
体外培养心肌细胞,用100 TCID50柯萨奇B3病毒(CVB3)感染心肌细胞,建立病毒性心肌炎实验模型,然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞,观察心肌细胞的形态和搏动,用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白,四唑蓝比色法测定心肌细胞活性和病毒抑制率,流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。
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Results The patients with cardiac amyloidosis had characteristics of large atriums and hypertrophic ventricular walls in echocardiography. And they also had low limb lead voltage in ECG. The ratio of (SV5+RV1) and left ventricle crossing area of all patients were obviously lower than 1.5. Biopsy behaved staining of congo red. And most patients had renal, dermatic and gastrointestinal involvements.
结果 心脏超声心动图表现为患者左、右心房增大,心室肥厚,心腔正常;心电图表现为肢体导联低电压,心电图电压(SV5+RV1)与左心室横截面积CSA比值均明显小于1.5;心外活检表现为刚果红染色阳性,且大部分患者合并肾脏、皮肤及消化系统损害。
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The histochemistry staining of cytokeratin, vimentin, alkaline phosphatase were positive and that of α-actin,Ⅷ Ag, fibronectin and desmine were negative.
细胞角蛋白、波形蛋白和碱性磷酸酶染色阳性,α-肌动蛋白、Ⅷ因子相关抗原、纤维连接蛋白和结蛋白染色阴性。
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The results from D6's morphological characters, colonial type, Gram's staining and a series of physiologic and biochemical experiments reveales that the strain of D6 is Desulfovibrio vulgaris Postgate and Campbell.
根据菌体形态、菌落特征、革兰氏染色和一系列生理生化试验结果,证明:D6菌属于脱硫弧菌属的种,名为普通脱硫弧菌Desulfovibrio vulgaris Postgate and Campbell。
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Results In dioestrous mice, many iNOS+ cells were found in the stroma of endometrium. There was no obvious differences between the immunized mice and the control mice. In the uterus on 3 day of pregnancy, however, there was obvious difference about the iNOS quantity in uterus between the immunized and the control mice. There was strong positive reaction on the epithelia of endometrium and the endometrial gland in the lamina propria in control mice, while the staining of iNOS in the uterus of SA-30 immunized mice was very weak, only a few of iNOS+ cells were observed in the myometrium.
结果 SA-30免疫后对间情期小鼠子宫内的iNOS没有明显影响,无论是免疫组还是对照组,在子宫内膜基质中均有大量阳性细胞分布,两者无明显差异;在妊娠第3d时,对照组的小鼠子宫中有较多iNOS表达,阳性标记主要集中在内膜上皮及固有膜中的子宫腺上,经SA-30免疫的小鼠子宫中,iNOS表达则显著减少,整个内膜标记极弱,只在子宫肌层内有少量iNOS阳性细胞分布。
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Results In dioestrous mice, many iNOS+ cells were found in the stroma of endometrium. There was no obvious differences between the immunized mice and the control mice. In the uterus on 3 day of pregnancy, however, there was obvious difference about the iNOS quantity in uterus between the immunized and the control mice. There was strong positive reaction on the epithelia of endometrium and the endometrial gland in the lamina propria in control mice, while the staining of iNOS in the uterus of SA-30 immunized mice was very weak, only a few of iNOS+ cells were observed in the myometrium.
结果 SA-30免疫后对间情期小鼠子宫内的iNOS没有明显瘙痒影响,无论是免疫组还是对照组,在子宫内膜基质中均有大量阳性偏瘫细胞分布,两者无明显瘙痒差异;在妊娠第3d时,对照组的小鼠子宫中有较多iNOS表达,阳性标记主要集中在内膜上皮及固有膜中的子宫腺上,经SA-30免疫的小鼠子宫中,iNOS表达则显著减少,整个内膜标记极弱,只在子宫肌层内有少量iNOS阳性偏瘫细胞分布。
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Immunocytochemical staining was used to identify sarcomeric actin and intercalated disc-like structure when the cells were cultured for additional 1 week, 2 or 3 weeks, respectively. RESULTS: Sarcomeric actin positive cells were observed in 1 week, 2 or 3 weeks after 5-aza treatment. Some cells stained positive for connexin43 at 1 week and 2 weeks after 5-aza treatment, with brown pellet located dispersively around nucleus.
结果: 5-aza处理后1周、2周、3周均可见横纹肌肌动蛋白染色阳性细胞。5-aza处理后1周及2周连结蛋白43在部分细胞上表达:呈核周散在分布的棕黄色颗粒,5-aza处理后3周连结蛋白43 在细胞密度大的区域呈核周聚集成线形分布的棕黄色颗粒,个别细胞间可见此结构,类似正常心肌的闰盘结构。
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Double fluorescent immunohistochemical staining revealed close apposition of VIP-, PACAP-, and vasopressin-immunoreactive terminals with tyrosine hydroxylase-ir neurons within the dorsomedial arcuate nucleus. We were then of interest to learn from where VIP-, PACAP- and AVP-containing neurons that project to the ARN originate.
经由双重免疫萤光染色法,可以在弓状核的背中区,具酪胺酸羟化酶免疫活性的细胞旁,发现有VIP、PACAP及血管加压素免疫活性的神经末稍的分布及并置,显示确有VIP,PACAP及AVP之神经元投射至弓状核。
- 推荐网络例句
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This one mode pays close attention to network credence foundation of the businessman very much.
这一模式非常关注商人的网络信用基础。
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Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.
扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。
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There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.
双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。