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Methods S-P immunohistochemical staining was used to detec t the expression of VEGF-C and its reˉceptor Flt-4in10neighboring noncancerous tissue and60NSCLC cases of non-small cell lung cancer and32lymph nodes cases with lymph nodes metastasis of non-small cell lung cancer.
采用免疫组化S-P法检测60例NSCLC原发灶组织、10例癌旁组织、32例伴有肺癌转移的淋巴结组织中VEGF-C、Flt-4的表达,并将结果与肺癌的临床病理特征进行分析。
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Methods S-P immunohistochemical staining was used to detect the expression of VEGF-C and its reˉceptor Flt-4in10neighboring noncancerous tissue and60NSCLC cases of non-small cell lung cancer and32lymph nodes cases with lymph nodes metastasis of non-small cell lung cancer.
目的 探讨血管内皮生长因子C及其受体Flt-4在人非小细胞肺癌组织中的表达及其与淋巴结转移等临床病理特征的关系方法采用免疫组化S-P法检测60例NSCLC原发灶组织、10例癌旁组织、32例伴有肺癌转移的淋巴结组织中VEGF-C、Flt-4的表达,并将结果与肺癌的临床病理特征进行分析。
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objective effects of plant tannins,chebulinic acid and tellimagrandin i on chemically induced hemoglobin synthesis in k562 cells were investigated.methods the hemoglobin synthesis situation was assayed with benzidine staining.erythroid antigens glycophorin a expression on the surface of k562 cells was labeled by direct immunofluorescence using fluorescein isothiocyanate-conjugated anti-gpa antibodies.then flow cytometric analysis was performed to detect gpa expression levels on surface of the cells.results both chebulinic acid and tellimagrandin i could inhibit the hemoglobin synthesis of butyric acid and hemin-treated k562 cells in a concentration-dependant manner.however,the ba-induced k562 cells were more sensitive to two tannins than heroin-induced cells.conclusion chebulinic acid and tellimagrandin i have inhibitory effect on the erythroid differentiation.
目的 研究可水解单宁诃子酸和特里马素i对氯化高铁血红素和丁酸钠诱导k562细胞红系分化的影响。方法四甲基偶氮噻唑蓝法分析诃子酸和特里马素i对k562细胞生长的影响,联苯胺染色法检测血红蛋白合成情况,应用免疫荧光和流式细胞术检测血型糖蛋白a在细胞表面的表达。结果诃子酸和特里马素i在0.04~0.09?mmol/l浓度下均可显著抑制k562细胞的生长增殖。2种单宁化合物(0.002~0.01?mmol/l)可显著抑制丁酸钠诱导的k562细胞血红蛋白合成,2种单宁化合物(0.01~0.05?mmol/l)还可显著抑制氯化高铁血红素诱导的血红蛋白合成,特里马素i(0.01?mmol/l)还抑制丁酸钠诱导的gpa在细胞表面表达。结论诃子酸和特里马素i对红系分化有抑制作用。
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Some of the steps of maintenance for a log cabin include staining of the interior and exterior wood, chinking repair and replacement, borate treatment to safeguard against pests, stay dry, chemical strip and cob blasting.
一些步骤,包括维修记录机舱内外的染色木材、chinking维修和更换,以防止病虫害硼酸待遇,逗留干燥、化学、芯地带爆破。
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The staining sections of the nuclear antigen of the gallbladder mucosal proliferation cell in adult cholecystic diseased patients with or without pancreticobiliary maljunction were observed to compare the difference of the pathological morphology.
对照观察伴有胰胆管合流异常与不伴胰胆管合流异常之成人胆囊疾病患者中的胆囊黏膜增殖细胞核抗原染色切片,以比较其病理形态的差别。
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The presence of the t(15;19) chromosomal translocation in the chondroid component was confirmed by fluorescence in situ hybridization analysis and immunohistochemical staining, indicating mesenchymal transdifferentation of the tumor.
荧光原位杂交实验和免疫组化染色证实其软骨样成分发生了t(15;19)染色体易位,由此表明该例肿瘤发生了间叶组织转分化。
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RESULTS: Four weeks following repair, CT showed that callus formation appeared in coronal skull defects. 3D reconstruction still demonstrated some defects. Hematoxylin and eosin staining displayed defects were fibrous connective tissue, with the presence of chondroid tissues.
结果:修复后4周,冠状位CT显示颅骨缺损区可见骨痂形成,三维重建仍显示有缺损;标本苏木精-伊红染色显示缺损为纤维结缔组织,并有软骨样组织形成,Mallory三色染色标本呈淡蓝色。
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EMA staining was positive in 100% of chordoid gliomas, 94% of chordomas, 90% of chordoid meningiomas, and 25% of extraskeletal myxoid chondrosarcomas.
EMA表达于100%例脊索样胶质瘤、94%例脊索瘤、90%例脊索样脑膜瘤、25%例骨外黏液样软骨肉瘤。
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In this study, immunohistochemical staining was performed with antibodies against D2-40, S100, pankeratin, epithelial membrane antigen, brachyury, and glial fibrillary acidic protein in 4 cases of chordoid glioma, 6 skeletal myxoid chondrosarcomas, 10 chordoid meningiomas, 16 extraskeletal myxoid chondrosarcoma, 18 chordomas, 22 low-grade chondrosarcomas, and 27 enchondromas.
本研究中,我们给4例脊索样胶质瘤、6例骨的黏液样软骨肉瘤、10脊索样脑膜瘤、16例骨外黏液样软骨肉瘤、18例脊索瘤、22例低级别软骨肉瘤和27例内生性软骨瘤做了D2-40、S100、pankeratin、上皮膜抗原、brachyury和胶质纤维酸性蛋白的免疫组化染色。
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Methods Damage zone and minimum safe distance were measured in vitro on chick chorioallantoic membrane and in vivo on rabbit retina with the use of propidium iodide staining.
应用propidium碘染色的方法,在体外实验中,测量鸡绒毛膜尿囊膜的损伤区和最小安全距离,在体内实验中,测量兔视网膜的损伤区和最小安全距离。
- 推荐网络例句
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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.
曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。
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The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.
稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。
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When I was in school, the rabbi explained everythingin the Bible two different ways.
当我上学的时候,老师解释《圣经》用两种不同的方法。