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The CCND2 expression cell proliferation, cell cycle and cell apopotosis of the transfected LP-1 cells were studied by RT-PCR, trypanosome staining, flow cytometry and annexin V assay.

设计、合成CCND2短发夹RNA双链DNA模板，构建表达质粒pGenesil-2／CCND2，转染LP-1细胞，以RT—PCR检测对CCND2表达的抑制作用，以锥虫蓝染色计数和MTI检测细胞增殖，以Annexin V检测细胞凋亡，流式细胞术检测细胞周期。

The L-form of tubercle bacillus can be detected by PCR technique and IK staining, with high positive rates and high sensitivity.

PCR技术及IK染色，检测抗酸菌L型的阳性率及灵敏度均较高。

The phenotype, calcium tuberculation and the expression of alkaline phosphatase were studied with alizarin red staining and modified Gomori Ca-Co assays respectively.

采用碱性磷酸酶Gomori 钙钴法染色、钙结节茜素红法染色及Ⅰ型胶原免疫组织化学染色方法进行鉴定成骨细胞生物学特性。

Paul Ehrlich refines the use of the dye methylene blue in bacteriological staining and uses it to stain the tubercule bacillus.

Paul Ehrlich优化了美蓝染料在细菌染色中的使用，用美蓝给tubercule bacillus。

The results showed that (1) the positive staining of ChAT was obviously observed in the cells of the three kinds of human pituitary adenoma, however, it was lower than that in normal human pituitary gland;(2) ACh had a similar effect on the proliferation of the three kinds of human pituitary adenoma cells. ACh at 0.1～10 μmol/L decreased the ［3H］TdR incorporation and the MTT A value in a dose-dependent manner. At the same time, ACh decreased the ratio of S or G2 phase pituitary adenoma cells significantly, but increased the ratio of G1 phase pituitary tumour cells markedly;(3) the effect of acetylcholine on the proliferation of human pituitary adenoma cells was inhibited by atropine, but not by tubocurarine;(4) ACh had no effect on the apoptosis of human pituitary adenoma cells cultured in vitro.

结果发现：(1)三种垂体腺瘤细胞中均有胆碱酯酶的表达，但明显少于正常垂体；(2)ACh对三种类型的人垂体腺瘤细胞增殖代谢的影响相类似，不同浓度的ACh能明显抑制体外培养的三种人垂体腺瘤细胞的增殖，呈明显的剂量效应关系，同时ACh能减少垂体腺瘤细胞进入S、G2期的细胞比例，而使处于G1期的细胞比例增加；(3)ACh的这种作用可被阿托品阻断，但不受筒箭毒的影响；(4)ACh对体外培养的三种人垂体腺瘤细胞凋亡无明显影响。

HE staining was made in mucosa of the media turbinate and compared with the results from the normal control group.

用HE染色中鼻甲黏膜，与空白对照组比较，观察其余3组大鼠鼻黏膜的病理的改变，并侧量黏膜的厚度、白细胞计数。

The distribution and morphology of argyrophil cells in intestine of Coturnix coturnix were observed and studied by Grimelius s tver staining.

本文采用Grimelius银染法对鹌鹑肠道嗜银细胞的分布及形态进行了观察和研究。

Objective: To verify whether hot PBS washing after the enzymatic reaction between tyramine and horseradish peroxidase can improve the sensitivity and specificity of catalyzed signal amplification. Methods: Using 7 different types of primary antibodies and 2 tomor microarray system, we carried out EnVision, LSAB, Standard CSA, and modified CSA staining sod compared their sensitivities and specificities. Results: The modified CSA method was the moat sensitive one among the 4 methods.

目的：探讨酪胺与过氧化物酶发生酶促反应后，用热缓冲液洗以进一步提高和改善催化信号放大法的敏感性和特异性方法：应用7种不同类型的一抗，2张不同肿瘤的组织芯片，同时进行EnVision法、LsAB法、标准CSA法和改进CSA法染色，比较敏感性和特异胜。

MAIN OUTCOME MEASURES: At 15 days after model preparation, one rat was obtained from each group to underdone morphological observation. At 21 and 30 days after model preparation, 8 rats were killed in each group, hematoxylin-eosin staining and immunohistochemical method were used to detect the expression of the proliferating cell nuclear antigen, and to calculate fibroblast proliferation index.

主要观察指标：于造模后15 d各组取1只大鼠行组织形态学观察；造模后21，30 d，每组各处死8只大鼠，取材后，行苏木精-伊红染色，免疫组织化学检测增殖细胞核抗原的表达，并计算成纤维细胞的增殖指数。

RESULTS: The AF cells cultured in different substrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on flexible silicone membranes and in plastic plates. The cells growing in different substrates expressed the same levels of collagen typeⅠ,Ⅱ and aggrecan mRNA and integrin β1. AF cells grew well on silicone membranes.

结果：种植于不同基质的纤维环细胞形态学观察无明显差别；在两种基质上的纤维环细胞甲苯胺蓝染色无差异；Ⅰ，Ⅱ型胶原及聚集蛋白聚糖mRNA及细胞膜整合素β1的表达亦无明显差异；纤维环细胞可以黏附于硅橡胶膜上生长。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。